GAPDH antibody | AbD22549_hIgG1
7.5 µl of each IP was loaded onto an AnykD™ Criterion™ TGX Stain-Free™ gel. 25.7µl Jurkat whole cell lysate (WCL) was run in lanes 5 & 9 while Precision Plus Protein™ Prestained Standards were run in lane 1.
Rabbit anti GAPDH antibody (VPA00187) was used at 1/1000 in lanes 1-5. HRP conjugated Tidyblot™ (STAR209P) was used at 1/200 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to GAPDH (molecular weight 37 kDa).
Human anti GAPDH antibody, clone AbD22549_hIgG1 recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a 335 amino acid multifunctional protein. The main function of GAPDH is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). It is a major glycolytic enzyme within the cytosol and is also involved in a number of intracellular processes, including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair. It has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death.
GAPDH has been shown to bind to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al. 1996).
Since GAPDH is constitutively expressed in almost all tissues at high levels, this antibody is recommended for use as a loading control for western blotting.
GAPDH has been shown to bind to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al. 1996).
Since GAPDH is constitutively expressed in almost all tissues at high levels, this antibody is recommended for use as a loading control for western blotting.
Product Details
- Target Species
- Human
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Recombinant human GAPDH
- Affinity
- The monovalent intrinsic affinity of AbD22549 was measured as KD = 2 nM by real time, label free molecular interaction analysis on immobilized HSPA4
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04406
- Entrez Gene
- GAPDH
- GO Terms
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0048471 perinuclear region of cytoplasm
- GO:0051287 NAD binding
- GO:0051402 neuron apoptosis
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of GAPDH antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/50 | 1/800 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Western Blotting
- Anti GAPDH antibody detects a band of approximately 36 kDa in Jurkat cell lysate under reducing conditions
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
|
|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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