GAPDH antibody | AbD22549_hIgG1

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Human anti GAPDH

Product Type
Monoclonal Antibody
Clone
AbD22549_hIgG1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA272 IP WB 0.1 mg
Human anti GAPDH antibody, clone AbD22549_hIgG1 recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a 335 amino acid multifunctional protein. The main function of GAPDH is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). It is a major glycolytic enzyme within the cytosol and is also involved in a number of intracellular processes, including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair. It has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death.

GAPDH has been shown to bind to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al. 1996).

Since GAPDH is constitutively expressed in almost all tissues at high levels, this antibody is recommended for use as a loading control for western blotting.

Product Details

Target Species
Human
Product Form
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant human GAPDH
Affinity
The monovalent intrinsic affinity of AbD22549 was measured as KD = 2 nM by real time, label free molecular interaction analysis on immobilized HSPA4
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
12 months from date of despatch

More Information

UniProt
P04406 Related reagents
Entrez Gene
GAPDH Related reagents
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of GAPDH antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/50 1/800
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Western Blotting
Anti GAPDH antibody detects a band of approximately 36 kDa in Jurkat cell lysate under reducing conditions

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*