GAPDH antibody | AbD22549_hIgG1
GAPDH has been shown to bind to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al. 1996).
Since GAPDH is constitutively expressed in almost all tissues at high levels, this antibody is recommended for use as a loading control for western blotting.
- Target Species
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Recombinant human GAPDH
- The monovalent intrinsic affinity of AbD22549 was measured as KD = 2 nM by real time, label free molecular interaction analysis on immobilized HSPA4
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
- 12 months from date of despatch
- P04406 Related reagents
- Entrez Gene
- GAPDH Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0048471 perinuclear region of cytoplasm
- GO:0051287 NAD binding
- GO:0051402 neuron apoptosis
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- For research purposes only
Applications of GAPDH antibody
|Application Name||Verified||Min Dilution||Max Dilution|
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Western Blotting
- Anti GAPDH antibody detects a band of approximately 36 kDa in Jurkat cell lysate under reducing conditions