FTO antibody | OTI4A1

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Mouse anti FTO

Product Type
PrecisionAb Monoclonal
Clone
OTI4A1
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00723
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human FTO antibody recognizes FTO, also known as AlkB homolog 9, alpha-ketoglutarate-dependent dioxygenase FTO, fat mass and obesity-associated protein and protein fto.

FTO is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of FTO is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes (provided by RefSeq, Jul 2011).

Mouse anti Human FTO antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti FTO detects a band of approximately 60 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
HEK293-derived full length recombinant protein of human FTO

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q9C0B1 Related reagents
Entrez Gene
FTO Related reagents
GO Terms
GO:0005634 nucleus
GO:0006307 DNA dealkylation involved in DNA repair
GO:0008198 ferrous iron binding
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0035515 oxidative RNA demethylase activity
GO:0035516 oxidative DNA demethylase activity
GO:0035552 oxidative single-stranded DNA demethylation
GO:0035553 oxidative single-stranded RNA demethylation
GO:0042245 RNA repair
GO:0043734 DNA-N1-methyladenine dioxygenase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of FTO antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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