FSCN1 antibody | CPTC29
Mouse anti Human FSCN1 antibody recognizes Fascin, also known as 55 kDa actin-bundling protein, fascin homolog 1, actin-bundling protein, singed-like (fascin homolog, sea urchin).
The FSCN1 gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of FSCN1 is known to be regulated by several microRNAs, and overexpression of FSCN1 may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of FSCN1 is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of FSCN1 is located on the long arm of chromosome 15 (provided by RefSeq, Sep 2011).
Mouse anti Human FSCN1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The FSCN1 gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of FSCN1 is known to be regulated by several microRNAs, and overexpression of FSCN1 may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of FSCN1 is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of FSCN1 is located on the long arm of chromosome 15 (provided by RefSeq, Sep 2011).
Mouse anti Human FSCN1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti FSCN1 detects a band of approximately 55 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein of human FSCN1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q16658 Related reagents
- Entrez Gene
- FSCN1 Related reagents
- GO Terms
- GO:0001725 stress fiber
- GO:0030674 protein binding, bridging
- GO:0051015 actin filament binding
- GO:0051017 actin filament bundle assembly
- GO:0005737 cytoplasm
- GO:0008144 drug binding
- GO:0008283 cell proliferation
- GO:0016477 cell migration
- GO:0030054 cell junction
- GO:0030175 filopodium
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of FSCN1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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