Fibrinogen antibody | AB10-3C12

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Mouse anti Human Fibrinogen:Biotin

Product Type
Monoclonal Antibody
Clone
AB10-3C12
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6191B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human fibrinogen antibody, clone AB10-3C12 recognizes fibrinogen, a 340 kDa glycoprotein secreted by the liver which circulates in plasma as a soluble homodimer. The identical monomer subunits are linked by 17 disulfide bond and each subunit is composed of 3 polypeptide chains referred to as alpha, beta and gamma (Tiscia & Margaglione 2018).

In response to bleeding, the coagulation cascade is activated which results in the conversion of fibrinogen to fibrin. The resulting fibrin spontaneously forms polymers creating an insoluble gel which combines with platelets to form a spongy mass. This mass is the blood clot which subsequently hardens to prevent further bleeding from the blood vessel (Tiscia & Margaglione 2018). The impairment of this conversion from fibrinogen to fibrin is associated with a range of pathologies including coagulopathies, ischemic stroke and obstetrical complications. It is also implicated in various neurological diseases such as Alzheimer’s, multiple sclerosis and traumatic central nervous system injury (Petersen et al. 2018).

The biotinylated Mouse anti Human fibrinogen antibody, clone AB10-3C12 (MCA6191B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human fibrinogen antibody, clone AB05-1F11 (MCA6190GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Fibrinogen purified from human serum
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P02679
P02671
P02675
Entrez Gene
FGA
FGB
FGG
GO Terms
GO:0002576 platelet degranulation
GO:0030674 protein binding, bridging
GO:0005102 receptor binding
GO:0007165 signal transduction
GO:0005577 fibrinogen complex
GO:0009897 external side of plasma membrane
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043499 eukaryotic cell surface binding
GO:0051258 protein polymerization
GO:0051592 response to calcium ion
GO:0051087 chaperone binding
GO:0005625 soluble fraction
Regulatory
For research purposes only

Applications of Fibrinogen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Fibrinogen MCA6190GA E 0.1 mg loader

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