EIF4E antibody

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Mouse anti EIF4E

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00452
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human EIF4E antibody recognizes the eukaryotic translation initiation factor 4E, also known as eIF-4E, eIF-4F 25 kDa subunit, eukaryotic translation initiation factor 4E-like 1 or mRNA cap-binding protein.

The protein encoded by the EIF4E gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for EIF4E (provided by RefSeq, Jan 2012).

Mouse anti Human EIF4E antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EIF4E detects a band of approximately 28 kDa in Jurkat cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
EIF4E recombinant protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P06730 Related reagents
Entrez Gene
EIF4E Related reagents
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005515 protein binding
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0000339 RNA cap binding
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0005845 mRNA cap binding complex
GO:0006406 mRNA export from nucleus
GO:0006417 regulation of translation
GO:0008286 insulin receptor signaling pathway
GO:0016281 eukaryotic translation initiation factor 4F complex
GO:0044419 interspecies interaction between organisms
GO:0045931 positive regulation of mitotic cell cycle
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EIF4E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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