EIF4E antibody
Mouse anti EIF4E
Mouse anti-human EIF4E antibody recognizes the eukaryotic translation initiation factor 4e, also known as eIF-4E, eIF-4F 25 kDa subunit or mRNA cap-binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.
EIF4E Antibody with Control Lysate
Mouse anti-human EIF4E antibody recognizes the eukaryotic translation initiation factor 4e, also known as eIF-4E, eIF-4F 25 kDa subunit or mRNA cap-binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00452 | WB | 100 µl | ||||
| VMA00452KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human EIF4E antibody recognizes the eukaryotic translation initiation factor 4E, also known as eIF-4E, eIF-4F 25 kDa subunit, eukaryotic translation initiation factor 4E-like 1 or mRNA cap-binding protein.
The protein encoded by the EIF4E gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for EIF4E (provided by RefSeq, Jan 2012).
Mouse anti Human EIF4E antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the EIF4E gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for EIF4E (provided by RefSeq, Jan 2012).
Mouse anti Human EIF4E antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- EIF4E recombinant protein
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P06730 Related reagents
- Entrez Gene
- EIF4E Related reagents
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
- GO:0000339 RNA cap binding
- GO:0003743 translation initiation factor activity
- GO:0005829 cytosol
- GO:0005845 mRNA cap binding complex
- GO:0006406 mRNA export from nucleus
- GO:0006417 regulation of translation
- GO:0008286 insulin receptor signaling pathway
- GO:0016281 eukaryotic translation initiation factor 4F complex
- GO:0044419 interspecies interaction between organisms
- GO:0045931 positive regulation of mitotic cell cycle
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of EIF4E antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti EIF4E detects a band of approximately 28 kDa in Jurkat cell lysate
- Western Blotting
- Anti EIF4E detects a band of approximately 28 kDa in Jurkat cell lysate
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
