EIF4E antibody

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Mouse anti EIF4E

Mouse anti-human EIF4E antibody recognizes the eukaryotic translation initiation factor 4e, also known as eIF-4E, eIF-4F 25 kDa subunit or mRNA cap-binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.

EIF4E Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00452	WB	100 µl
VMA00452KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human EIF4E antibody recognizes the eukaryotic translation initiation factor 4E, also known as eIF-4E, eIF-4F 25 kDa subunit, eukaryotic translation initiation factor 4E-like 1 or mRNA cap-binding protein.

The protein encoded by the EIF4E gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for EIF4E (provided by RefSeq, Jan 2012).

Mouse anti Human EIF4E antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti EIF4E detects a band of approximately 28 kDa in Jurkat cell lysate

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from ascites

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

EIF4E recombinant protein

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P06730 Related reagents
Entrez Gene: EIF4E Related reagents
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0005515 protein binding; GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening; GO:0000339 RNA cap binding; GO:0003743 translation initiation factor activity; GO:0005829 cytosol; GO:0005845 mRNA cap binding complex; GO:0006406 mRNA export from nucleus; GO:0006417 regulation of translation; GO:0008286 insulin receptor signaling pathway; GO:0016281 eukaryotic translation initiation factor 4F complex; GO:0044419 interspecies interaction between organisms; GO:0045931 positive regulation of mitotic cell cycle
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of EIF4E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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