EGF R antibody | ICR10
Rat anti Human EGF Receptor:RPE
- Product Type
- Monoclonal Antibody
- Clone
- ICR10
- Isotype
- IgG2a
- Specificity
- EGF R
Rat anti Human EGF Receptor antibody, clone ICR10 recognizes the human epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. Rat anti Human EGF Receptor antibody, clone ICR10 binds to epitope B from EGFR (Lottaz et al. 2010 and Modjtahedi et al. 1993) and has an affinity of 6.7 x 10-9 M. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Extracellular domain of human EGF-receptor from head and neck carcinoma.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control:RPE | MCA6005PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Rat IgG2a Negative Control:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for EGF R antibody
-
Modjtahedi, H. et al. (1993) Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor.
Cell Biophys. 22 (1-3): 129-46. -
Gilcrease, M.Z. et al. (2009) Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer.
J Exp Clin Cancer Res. 28: 67. -
Lottaz,C. et al. (2010) Transcriptional Profiles of CD133+ and CD133- Glioblastoma-Derived Cancer Stem Cell Lines Suggest Different Cells of Origin
Cancer Res. 70: 2030-40. -
Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
Br J Cancer. 106 (5): 883-8. -
Grinberg, O. et al. (2013) Antibody modified Bovine Serum Albumin microspheres for targeted delivery of anticancer agent Gemcitabine
Polymers for Advanced Technologies. 24 (3): 294-299. -
Khelwatty, S.A. et al. (2015) Acquired resistance to anti-EGFR mAb ICR62 in cancer cells is accompanied by an increased EGFR expression, HER-2/HER-3 signalling and sensitivity to pan HER blockers.
Br J Cancer. 113 (7): 1010-9. -
Tilburgs, T. et al. (2015) The HLA-G cycle provides for both NK tolerance and immunity at the maternal-fetal interface.
Proc Natl Acad Sci U S A. 112 (43): 13312-7. -
Tilburgs, T. et al. (2015) Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes.
Proc Natl Acad Sci U S A. 112 (23): 7219-24.
View The Latest Product References
Further Reading
-
Modjtahedi, H. & Dean, C. (1994) The receptor for EGF and its ligands - expression, prognostic value and target for therapy in cancer (review).
Int J Oncol. 4 (2): 277-96.
MCA1784PE
154823 156702If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up