Collagen I/II/III antibody | NLI/22

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Anti Human Collagen I/II/III Antibody, clone NLI/22 thumbnail image 1

Anti Human Collagen I/II/III Antibody, clone NLI/22 gallery image 1 — Sandwich ELISA analysis of human collagen using Mouse anti Human collagen I/II/III antibody, clone NLI/22 (**2150-2203**) as a capture reagent and biotinylated Mouse anti Human collagen I antibody, (131008) as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (This image is from an open access article distributed under terms of a Creative Commons Attribution License. >Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4

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Mouse anti Human Collagen I/II/III

Product Type: Monoclonal Antibody
Clone: NLI/22
Isotype: IgM
Specificity: Collagen I/II/III

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Product Code Applications Pack Size List Price Your Price Qty

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2150-2203 Datasheet Datasheet SDS SDS	C E	1 ml		Log in	Get Quote
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Rabbit anti Human collagen I/II/III antibody reacts with collagens type I, II and III. It does not react with human collagen types IV, V or VI, human plasma proteins or albumin from various species.

Target Species: Human
Product Form: Purified IgM - lyophilized
Reconstitution: Use 1 ml sterile distilled water to give a concentration of 0.1 mg/ml.
Preparation: Gel Filtration chromatography from ascites
Buffer Solution: 0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
Preservative Stabilisers: 1% Mannitol
1% Dextran
Immunogen: Human placental collagen type 1.
Regulatory: For research purposes only
Guarantee: Guaranteed until date of expiry. Please see product label.

Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/500	1/2000
Immunohistology - Frozen		1/5	1/20

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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