Collagen I/II/III antibody | NLI/22

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Anti Human Collagen I/II/III Antibody, clone NLI/22 gallery image 1 — Sandwich ELISA analysis of human collagen using Mouse anti Human collagen I/II/III antibody, clone NLI/22 (**2150-2203**) as a capture reagent and biotinylated Mouse anti Human collagen I antibody, (131008) as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4

Mouse anti Human Collagen I/II/III

Product Type: Monoclonal Antibody
Clone: NLI/22
Isotype: IgM

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
2150-2203	C E			1 ml

Summary
Secondary Antibodies

Rabbit anti Human collagen I/II/III antibody reacts with collagens type I, II and III. It does not react with human collagen types IV, V or VI, human plasma proteins or albumin from various species.

Product Details

Target Species: Human
Product Form: Purified IgM - lyophilised
Reconstitution: Use 1 ml sterile distilled water to give a concentration of 0.1 mg/ml.
Preparation: Gel Filtration chromatography
Buffer Solution: Contains dextran, mannitol and salts.
Preservative Stabilisers: None present
Immunogen: Human placental collagen type 1.

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P02452 Related reagents; P08123 Related reagents; P02458 Related reagents; P02461 Related reagents
Entrez Gene: COL1A1 Related reagents; COL2A1 Related reagents; COL3A1 Related reagents; COL1A2 Related reagents
Regulatory: For research purposes only

Applications of Collagen I/II/III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/500	1/2000
Immunohistology - Frozen		1/5	1/20

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgM:FITC	HCA040F	0.1 mg	F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)	STAR138A	1 ml	C E P WB
Goat anti Mouse IgM:RPE	STAR86PE	0.5 ml	F
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E

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