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Collagen I

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Sandwich ELISA analysis of human ferritin using Mouse anti Human collagen I/II/III antibody, clone NLI/22 (2150-2203) as a capture reagent and biotinylated Mouse anti Human collagen I antibody, (131008) as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4

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  • Native Human Collagen I
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-0030Edatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.
    • Intended Use
    • Target Species
    • Product Form
      Purified protein from human placenta - lyophilised
    • Reconstitution
      Use 0.05-0.5 M acetic acid, pH 2.5 at 4ºC.
    • Preparation
      Collagen from washed dissected tissue was dissolved in acetic acid after mild pepsin treatment. Collagen type I was then purified using differential salt precipitation.
    • Preservative Stabilisers
      None present.
    • Purity
    • Approx. Protein Concentrations
    • Molecular Weight
      300 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
      The human source was tested and found negative for HBsAg ans HIV-1/2 antibodies.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen I Formats

    Formats Applications Sizes available
    Collagen I : Purified E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

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