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CHD4 antibody | AB01/2B7

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siRNA Validated
IP-MS Validated
Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with CHD4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 36 second exposure. Arrow points to CHD4 (molecular weight 278 kDa).
Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CHD4 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti CHD4 antibody (VMA00619) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 59 second (chemiluminescent channel) and 10 second (rhodamine channel) exposure.
Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of CHD4 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CHD4 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.
Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 4
Immunoprecipitation of CHD4 from HEK293 whole cell lysate. The Input lane is 10% input; the CHD4-IP lane is the immunoprecipitation with the CHD4 antibody (2.5 µg, VMA00619); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using CHD4 antibody (1/1,000, VMA00619) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CHD4.

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WB IP

Mouse anti CHD4

Product Type
PrecisionAb Monoclonal
Clone
AB01/2B7
Isotype
IgG1
Format
Purified
Specificity
CHD4

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Product Code Applications Pack Size List Price Your Price Qty
VMA00619
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human CHD4 antibody recognizes CHD4, also known as chromodomain-helicase-DNA-binding protein 4.

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2014).

Mouse anti Human CHD4 antibody detects a band of 278 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti CHD4 antibody detects a band of approximately 278 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human CHD4 (aa 322-1731)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q14839
Entrez Gene
CHD4
GO Terms
GO:0005515 protein binding
GO:0000785 chromatin
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0006333 chromatin assembly or disassembly
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0006350 transcription
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0016581 NuRD complex

VMA00619

170517

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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