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CHD4 antibody | AB01/2B7

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Western Blot Validated
Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with CHD4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 36 second exposure. Arrow points to CHD4 (molecular weight 278 kDa).

Mouse anti CHD4

CHD4 antibody recognizes CHD4, also known as chromodomain-helicase-DNA-binding protein 4.

Product Type
PrecisionAb™ Monoclonal
Clone
AB01/2B7
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00619 WB 100 µl
Mouse anti Human CHD4 antibody recognizes CHD4, also known as chromodomain-helicase-DNA-binding protein 4.

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2014).

Mouse anti Human CHD4 antibody detects a band of 278 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti CHD4 antibody detects a band of approximately 278 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human CHD4 (aa 322-1731)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q14839 Related reagents
Entrez Gene
CHD4 Related reagents
GO Terms
GO:0005515 protein binding
GO:0000785 chromatin
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0006333 chromatin assembly or disassembly
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0006350 transcription
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0016581 NuRD complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CHD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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