CHD4 antibody | AB01/2B7
Mouse anti CHD4
CHD4 antibody recognizes CHD4, also known as chromodomain-helicase-DNA-binding protein 4.
CHD4 Antibody with Control Lysate
CHD4 antibody recognizes CHD4, also known as chromodomain-helicase-DNA-binding protein 4.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- AB01/2B7
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00619 | WB | 100 µl | ||||
| VMA00619KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human CHD4 antibody recognizes CHD4, also known as chromodomain-helicase-DNA-binding protein 4.
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2014).
Mouse anti Human CHD4 antibody detects a band of 278 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2014).
Mouse anti Human CHD4 antibody detects a band of 278 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti CHD4 antibody detects a band of approximately 278 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human CHD4 (aa 322-1731)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q14839 Related reagents
- Entrez Gene
- CHD4 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000785 chromatin
- GO:0003677 DNA binding
- GO:0003682 chromatin binding
- GO:0006333 chromatin assembly or disassembly
- GO:0004003 ATP-dependent DNA helicase activity
- GO:0005524 ATP binding
- GO:0006350 transcription
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0008270 zinc ion binding
- GO:0016568 chromatin modification
- GO:0016581 NuRD complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of CHD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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