CHD4 antibody | AB01/2B7

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Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CHD4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 36 second exposure. Arrow points to CHD4 (molecular weight 278 kDa).

Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CHD4 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti CHD4 antibody (**VMA00619**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 59 second (chemiluminescent channel) and 10 second (rhodamine channel) exposure.

Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of CHD4 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CHD4 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Anti CHD4 Antibody, clone AB01/2B7 (PrecisionAb Monoclonal Antibody) gallery image 4 — Immunoprecipitation of CHD4 from HEK293 whole cell lysate. The Input lane is 10% input; the CHD4-IP lane is the immunoprecipitation with the CHD4 antibody (2.5 µg, **VMA00619**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using CHD4 antibody (1/1,000, **VMA00619**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CHD4.

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WB IP

Mouse anti CHD4

Product Type: PrecisionAb Monoclonal
Clone: AB01/2B7
Isotype: IgG1
Format: Purified
Specificity: CHD4

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00619 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human CHD4 antibody recognizes CHD4, also known as chromodomain-helicase-DNA-binding protein 4.

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2014).

Mouse anti Human CHD4 antibody detects a band of 278 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti CHD4 antibody detects a band of approximately 278 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human CHD4 (aa 322-1731)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q14839
Entrez Gene: CHD4
GO Terms: GO:0005515 protein binding; GO:0000785 chromatin; GO:0003677 DNA binding; GO:0003682 chromatin binding; GO:0006333 chromatin assembly or disassembly; GO:0004003 ATP-dependent DNA helicase activity; GO:0005524 ATP binding; GO:0006350 transcription; GO:0006357 regulation of transcription from RNA polymerase II promoter; View More GO Terms; GO:0008270 zinc ion binding; GO:0016568 chromatin modification; GO:0016581 NuRD complex