CD66 antibody | rB08-7A10

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Mouse anti Human CD66:StarBright Blue 580

Product Type: Monoclonal Antibody
Clone: rB08-7A10
Isotype: IgG1
Specificity: CD66

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TZA079SBB580 Datasheet SDS SDS	F	100 Tests/0.5ml		Log in
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Anti Human CD66 Antibody, clone rB08-7A10 thumbnail image 8

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Anti Human CD66 Antibody, clone rB08-7A10 gallery image 1 — Human peripheral blood was stained with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) only (Figure A) or with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) and Anti-Human CD66 SBUV400 (**TZA079SBUV400**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 2 — Human peripheral blood was stained with Anti-Human CD10 SBY800 (**MCA1556SBY800**) only (Figure A) or with Anti-Human CD10 SBY800 (**MCA1556SBY800**) and Anti-Human CD66 SBUV665 (**TZA079SBUV665**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 3 — Human peripheral blood was stained with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) only (Figure A) or with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) and Anti-Human CD66 SBUV740 (**TZA079SBUV740**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 4 — Human peripheral blood was stained with Anti-Human CD10 SBY800 (**MCA1556SBY800**) only (Figure A) or with Anti-Human CD10 SBY800 (**MCA1556SBY800**) and Anti-Human CD66 SBV515 (**TZA079SBV515**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 5 — Human peripheral blood was stained with Anti-Human CD10 SBY800 (**MCA1556SBY800**) only (Figure A) or with Anti-Human CD10 SBY800 (**MCA1556SBY800**) and Anti-Human CD66 SBV610 (**TZA079SBV610**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the ZE5 Cell Analyzer.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 6 — Human peripheral blood was stained with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) only (Figure A) or with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) and Anti-Human CD66 SBV790 (**TZA079SBV790**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 7 — Human peripheral blood was stained with Anti-Human CD10 SBY800 (**MCA1556SBY800**) only (Figure A) or with Anti-Human CD10 SBY800 (**MCA1556SBY800**) and Anti-Human CD66 SBB615 (**TZA079SBB615**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 8 — Human peripheral blood was stained with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) only (Figure A) or with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) and Anti-Human CD66 SBB675 (**TZA079SBB675**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 9 — Human peripheral blood was stained with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) only (Figure A) or with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) and Anti-Human CD66 SBB765 (**TZA079SBB765**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD66 Antibody, clone rB08-7A10 gallery image 10 — Human peripheral blood was stained with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) only (Figure A) or with Anti-Human CD10 SBUV575 (**MCA1556SBUV575**) and Anti-Human CD66 SBY800 (**TZA079SBY800**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single cell granulocytes. Data were acquired on the **ZE5 Cell Analyzer**.

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Mouse anti Human CD66 antibody, clone rB08-7A10, is a recombinant monoclonal antibody that recognizes an epitope common to CD66 a, c, d and e, also known respectively as cell adhesion molecule CEACAM 1, 6, 3 and 5.

CD66 a, c, d and e are members of the carcinoembryonic antigen (CEA) family, which is involved in a range of cellular processes through homotypic and heterotypic interactions. CD66 a, c, d and e all have an extracellular region containing at least one domain homologous to the immunoglobulin variable domain. CD66 a and d are transmembrane proteins, whilst CD66 c and e associate to the cell membrane with GPI anchors. CD66 proteins each have a specific expression pattern. CD66a is broadly expressed and the only family member expressed on activated T cells. CD66a has been attributed to multiple cellular functions, including the modulation of adaptive and innate immune responses, roles in apoptosis, angiogenesis, and involvement in the development of 3D tissue structure. The binding of CD66a by viral and bacterial adhesins can inhibit the function of immune cells, providing a route for pathogens to potentially suppress immune response to infection.

CD66 proteins are associated with immune related diseases and are considered clinical biomarkers and promising therapeutic targets for various types of cancer. CD66a is involved in inflammatory bowel disease, with a protective role in models of colitis. CD66a is a biomarker in non-small cell lung cancer, pancreatic adenocarcinoma and melanoma. CD66e is approved by the FDA as a diagnostic marker for colon cancer.

Target Species: Human
Product Form: Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain and conjugated to StarBright Blue 580 – liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source: CHO cells
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: Recombinant CD66a Gln35-Gly428 expressed by mammalian cells
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Blue 580 475 582
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is covered by U.S Patent No. 10,150,841 and related U.S and foreign counterparts.

Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Blue 580	475	582

Store at +4°C. DO NOT FREEZE. This product should be stored undiluted.

This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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UniProt: P13688; P40198; P06731; P40199
Entrez Gene: CEACAM5; CEACAM1; CEACAM3; CEACAM6
GO Terms: GO:0005887 integral to plasma membrane; GO:0031225 anchored to membrane; GO:0016323 basolateral plasma membrane; GO:0016021 integral to membrane; GO:0007165 signal transduction; GO:0007267 cell-cell signaling