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CD62P antibody | Psel.KO.2.12

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Anti Human CD62P Antibody, clone Psel.KO.2.12 thumbnail image 1
Anti Human CD62P Antibody, clone Psel.KO.2.12 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B . FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse anti Human CD62P (MCA2420A647). All experiments performed on human blood gated on single platelets activated with thrombin, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human CD62P:RPE

Product Type
Monoclonal Antibody
Clone
Psel.KO.2.12
Isotype
IgG1
Specificity
CD62P

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Product Code Applications Pack Size List Price Your Price Qty
MCA2420PE
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Mouse anti Human CD62P antibody, clone Psel.KO.2.12 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein also known as P-selectin.

CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Mouse anti Human CD62P antibody, clone Psel.KO.2.12 inhibits P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells (Massaguer et al. 2000).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Rat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
CD62P transfected 300.19 cells.
Fusion Partners
Spleen cells from immunized CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Human Seroblock BUF070B F 200 Test
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Source Reference

  1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
    Tissue Antigens. 56 (2): 117-28.

References for CD62P antibody

  1. Massaguer, A. et al. (2002) Reactivity of CD62P workshop mAbs with resting and activated platelets from different animal species.
    In: Leucocyte Typing VII. Edited by Mason, D. et al. Oxford University Press, pp 342-3.
  2. Dasse, K.A. et al. (2007) Assessment of hydraulic performance and biocompatibility of a MagLev centrifugal pump system designed for pediatric cardiac or cardiopulmonary support.
    ASAIO J. 53 (6): 771-7.
  3. Johnson, C.A. Jr. et al. (2008) Flow cytometric assays for quantifying activated ovine platelets.
    Artif Organs. 32: 136-45.
  4. Tran GT et al. (2010) Membrane attack complex of complement is not essential for immune mediated demyelination in experimental autoimmune neuritis.
    J Neuroimmunol. 229 (1-2): 98-106.
  5. Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer.
    Biomaterials. 31: 2736-45.
  6. Johnson, C.A. Jr. et al. (2011) Biocompatibility assessment of the first generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (1): 9-21.
  7. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.
  8. Ding, J. et al. (2015) Quantification of Shear-Induced Platelet Activation: High Shear Stresses for Short Exposure Time.
    Artif Organs. 39 (7): 576-83.
    9. View The Latest Product References
  9. Foruzanmehr, M. et al. (2014) Nano-structure TiO2 film coating on 316L stainless steel via sol-gel technique for blood compatibility improvement.
    Nanomedicine Journal 1 (3): 128-36.

Flow Cytometry

Synonyms
P-SELECTIN
RRID
AB_609600
UniProt
P16109
Entrez Gene
SELP
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding

MCA2420PE

150218 INN0414

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