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CD62E/CD62P antibody | 1.2B6

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Anti Human CD62E/CD62P Antibody, clone 1.2B6 thumbnail image 1 Anti Human CD62E/CD62P Antibody, clone 1.2B6 thumbnail image 2
Anti Human CD62E/CD62P Antibody, clone 1.2B6 gallery image 1
Figure A. FITC conjugated Mouse anti Rat CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Rat CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse anti Human CD62E/CD62P (MCA883A647). All experiments performed on thrombin activated Human platelets gated on platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD62E/CD62P Antibody, clone 1.2B6 gallery image 2
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human CD61 (MCA2588F) and PE conjugated Mouse anti Human CD62E/CD62P (MCA883PE). All experiments performed on thrombin activated Human platelets gated on platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD62E/CD62P:RPE

Product Type
Monoclonal Antibody
Clone
1.2B6
Isotype
IgG1
Specificity
CD62E/CD62P

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Product Code Applications Pack Size List Price Your Price Qty
MCA883PE
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F 100 Tests loader
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Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens.

Although initially thought to recognize only human CD62E, more recent data (Goda et al. 2003) shows that Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 also recognizes human CD62P, binding to a common epitope shared by these members of the selectin family.

Clone 1.2B6 reacts with porcine E-selectin (CD62E) but not with porcine P-selectin (Stocker et al. 2000).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Human E-Selectin (ELAM-1).
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
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List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

Source Reference

  1. Wellicome, S.M. et al. (1990) A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide.
    J Immunol. 144 (7): 2558-65.

References for CD62E/CD62P antibody

  1. Thornhill, M.H. & Haskard, D.O. (1990) IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma.
    J Immunol. 145 (3): 865-72.
  2. Keelan, E.T. et al. (1994) Characterization of E-selectin expression in vivo with use of a radiolabeled monoclonal antibody.
    Am J Physiol. 266 (1 Pt 2): H278-90.
  3. Kyan-Aung, U. et al. (1991) Endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 mediate the adhesion of eosinophils to endothelial cells in vitro and are expressed by endothelium in allergic cutaneous inflammation in vivo.
    J Immunol. 146 (2): 521-8.
  4. Gómez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis.
    J Virol. 73: 2173-80.
  5. Goda, K. et al. (1999) Characterization of an apparently conserved epitope in E- and P-selectin identified by dual-specific monoclonal antibodies.
    Eur J Immunol. 29 (5): 1551-60.
  6. Stocker, C.J. et al. (2000) TNF-alpha, IL-4, and IFN-gamma regulate differential expression of P- and E-selectin expression by porcine aortic endothelial cells.
    J Immunol. 164: 3309-15.
  7. Vallée, I. et al. (2001) African swine fever virus infection of porcine aortic endothelial cells leads to inhibition of inflammatory responses, activation of the thrombotic state, and apoptosis.
    J Virol. 75: 10372-82.
  8. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther 321: 656-62.
    9. View The Latest Product References

Flow Cytometry

Synonyms
E-SELECTIN/P-SELECTIN
UniProt
P16581
P16109
Entrez Gene
SELP
SELE
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
GO:0007157 heterophilic cell-cell adhesion
GO:0002092 positive regulation of receptor internalization
GO:0002523 leukocyte migration involved in inflammatory response
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005901 caveola
GO:0005905 coated pit
GO:0007202 activation of phospholipase C activity
GO:0019722 calcium-mediated signaling
GO:0030029 actin filament-based process
GO:0030863 cortical cytoskeleton
GO:0034612 response to tumor necrosis factor
GO:0043274 phospholipase binding
GO:0048471 perinuclear region of cytoplasm
GO:0050727 regulation of inflammatory response
GO:0050901 leukocyte tethering or rolling
GO:0070555 response to interleukin-1

MCA883PE

150094

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