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CD59 antibody | YTH53.1

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Anti Human CD59 Antibody, clone YTH53.1 thumbnail image 1 Anti Human CD59 Antibody, clone YTH53.1 thumbnail image 2
Anti Human CD59 Antibody, clone YTH53.1 gallery image 1
Staining of human peripheral blood granulocytes with Rat anti Human CD59 (MCA715GT)
Anti Human CD59 Antibody, clone YTH53.1 gallery image 2
Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59 (MCA715G).

Rat anti Human CD59:RPE

Product Type
Monoclonal Antibody
Clone
YTH53.1
Isotype
IgG2b
Specificity
CD59

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Product Code Applications Pack Size List Price Your Price Qty
MCA715PE
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F 100 Tests loader
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Rat anti Human CD59 antibody, clone YTH53.1 recognizes the human CD59 cell surface antigen, also known as MAC-inhibitory protein, 1F5 antigen, 20 kDa homologous restriction factor, MEM43 antigen, membrane attack complex inhibition factor, membrane inhibitor of reactive lysis or protectin. CD59 is produced as a 103 amino acid proprotein, further processed to produce an 18-20 kDa GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes.

Mutation of the CD59 gene can result in the presentation of Hemolytic anemia, CD59-mediated, with or without polyneuropathy (HACD59) a disease characterized by infantile onset of a relapsing-remitting polyneuropathy manifest as limb muscle weakness, and hyporeflexia.

Rat anti Human CD59 antibody, clone YTH53.1 blocks the normal function of CD59 (Walsh et al. 1991).

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Human Peripheral Blood T cells.
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. We do not recommend using this antibody in lysed whole blood techniques due to inhibition of staining caused by CD59 expression on erythrocytes.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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Description Rat IgG2b Negative Control:RPE

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Source Reference

  1. Davies, A. et al. (1989) CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.
    J Exp Med. 170 (3): 637-54.

References for CD59 antibody

  1. Walsh, L.A. et al. (1991) Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement.
    Eur J Immunol. 21 (3): 847-50.
  2. Davies, A. et al. (1995) Identification of MIC 11 antigen as an epitope of the CD59 molecule.
    Immunology. 85 (2): 220-7.
  3. Hosokawa, M. et al. (2004) Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs.
    J Neuroinflammation. 1: 17.
  4. Ajona, D. et al. (2007) Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth.
    J Immunol. 178 (9): 5991-8.
  5. Ellison BS et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
    Cell Div. 2: 20.
  6. Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
    J Leukoc Biol. 86: 155-66.
  7. Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
    Invest Ophthalmol Vis Sci. 50: 3473-81.
  8. Zhu, C. et al. (2020) Isatuximab Acts Through Fc-Dependent, Independent, and Direct Pathways to Kill Multiple Myeloma Cells.
    Front Immunol. 11: 1771.
    9. View The Latest Product References

Functional Assays

Immunofluorescence

RRID
AB_321513
UniProt
P13987
Entrez Gene
CD59
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0007166 cell surface receptor linked signaling pathway
GO:0031362 anchored to external side of plasma membrane

MCA715PE

151681 INN0215

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