CD55 antibody | Bu14
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|Mouse anti Human CD55 antibody, clone Bu14 recognizes human CD55, also known as Decay Accelerating Factor (DAF). CD55 is a glycosylphosphatylinositol (GPI) anchored type I surface protein which prevents complement mediated lysis of autologous cells. CD55 functions to limit the formation and accelerate the decay of C3/C5-convertases from the classic and alternative pathway.
The molecular weight of CD55 varies between ~50 and 100 kDa dependent on cell type. Alternative transcripts can lead to soluble DAF, which is detectable in plasma, tears, saliva, urine and cerebrospinal fluid (Medof et al. 1987).
CD55 acts as a ligand for CD97 (Hamaan et al. 1998) and is an entry receptor for Echovirus (Bergelson et al. 1994) and Coxsackie B virus (Hafenstein et al. 2007). CD55 has been linked to many diseases including multiple sclerosis, rheumatoid arthritis and cancer development (Visser et al. 2002).
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD55 antibody
Loveland, B.E. and Szokolai, K. (1998) CD55 Workshop Report NL11
In: Leukocyte Typing VI, Eds Kishmoto, T. et al. Garland publishing . 519-520.
Ruiz-Argüelles, A. & Llorente, L. (2007) The role of complement regulatory proteins (CD55 and CD59) in the pathogenesis of autoimmune hemocytopenias.
Autoimmun Rev. 6 (3): 155-61.
Mikesch, J.H. et al. (2006) Decay-accelerating factor (CD55): a versatile acting molecule in human malignancies.
Biochim Biophys Acta. 1766 (1): 42-52.
- Entrez Gene
- GO Terms
- GO:0005576 extracellular region
- GO:0005887 integral to plasma membrane
- GO:0005625 soluble fraction
- GO:0006958 complement activation, classical pathway
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0031225 anchored to membrane
- GO:0045087 innate immune response
- GO:0045121 membrane raft
- GO:0045730 respiratory burst
- View More GO Terms
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