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CD55 antibody | Bu14

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Anti Human CD55 Antibody, clone Bu14 thumbnail image 1
Anti Human CD55 Antibody, clone Bu14 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA1568A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B . Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA1568A647) and FITC conjugated Mouse anti Human CD55 (MCA2659F). All experiments performed on Human peripheral blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human CD55:FITC

Product Type
Monoclonal Antibody
Clone
Bu14
Isotype
IgG1
Specificity
CD55

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Product Code Applications Pack Size List Price Your Price Qty
MCA2659F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD55 antibody, clone Bu14 recognizes human CD55, also known as Decay Accelerating Factor (DAF). CD55 is a glycosylphosphatylinositol (GPI) anchored type I surface protein which prevents complement mediated lysis of autologous cells. CD55 functions to limit the formation and accelerate the decay of C3/C5-convertases from the classic and alternative pathway.

The molecular weight of CD55 varies between ~50 and 100 kDa dependent on cell type. Alternative transcripts can lead to soluble DAF, which is detectable in plasma, tears, saliva, urine and cerebrospinal fluid (Medof et al. 1987).

CD55 acts as a ligand for CD97 (Hamaan et al. 1998) and is an entry receptor for Echovirus (Bergelson et al. 1994) and Coxsackie B virus (Hafenstein et al. 2007). CD55 has been linked to many diseases including multiple sclerosis, rheumatoid arthritis and cancer development (Visser et al. 2002).

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD55 antibody

  1. Loveland, B.E. and Szokolai, K. (1998) CD55 Workshop Report NL11
    In: Leukocyte Typing VI, Eds Kishmoto, T. et al. Garland publishing . 519-520.

Further Reading

  1. Ruiz-Argüelles, A. & Llorente, L. (2007) The role of complement regulatory proteins (CD55 and CD59) in the pathogenesis of autoimmune hemocytopenias.
    Autoimmun Rev. 6 (3): 155-61.
  2. Mikesch, J.H. et al. (2006) Decay-accelerating factor (CD55): a versatile acting molecule in human malignancies.
    Biochim Biophys Acta. 1766 (1): 42-52.

Synonyms
DAF
RRID
AB_2275855
UniProt
P08174
Entrez Gene
CD55
GO Terms
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0005625 soluble fraction
GO:0006958 complement activation, classical pathway
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0031225 anchored to membrane
GO:0045087 innate immune response
GO:0045121 membrane raft
GO:0045730 respiratory burst

MCA2659F

0411 154602

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