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CD55 antibody | 67

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Anti Human CD55 Antibody, clone 67 thumbnail image 1 Anti Human CD55 Antibody, clone 67 thumbnail image 2 Anti Human CD55 Antibody, clone 67 thumbnail image 3 Anti Human CD55 Antibody, clone 67 thumbnail image 4 Anti Human CD55 Antibody, clone 67 thumbnail image 5 Anti Human CD55 Antibody, clone 67 thumbnail image 6 Anti Human CD55 Antibody, clone 67 thumbnail image 7 Anti Human CD55 Antibody, clone 67 thumbnail image 8 Anti Human CD55 Antibody, clone 67 thumbnail image 9
Anti Human CD55 Antibody, clone 67 gallery image 1
Figure A. Pacific Blue conjugated Mouse anti Human CD3 (MCA463PB) and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. Pacific Blue conjugated Mouse anti Human CD3 (MCA463PB) and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD55 (MCA1614P647). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD55 Antibody, clone 67 gallery image 2
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse anti Human CD55 (MCA1614PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD55 Antibody, clone 67 gallery image 3
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse anti Human CD55 (MCA1614PE). All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD55 Antibody, clone 67 gallery image 4
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and Alexa Fluor 488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and Alexa Fluor 488 conjugated Mouse anti Human CD55 (MCA1614A488). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD55 Antibody, clone 67 gallery image 5
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463F) and FITC conjugated Mouse anti Human CD55 (MCA1614F). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD55 Antibody, clone 67 gallery image 6
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 700 conjugated Mouse IgG1 isotype control (MCA928A700). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 700 conjugated Mouse anti Human CD55 (MCA1614A700). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD55 Antibody, clone 67 gallery image 7
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse anti Human CD55 (MCA1614A647). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.
Anti Human CD55 Antibody, clone 67 gallery image 8
Published customer image:
Mouse anti Human CD55 antibody, clone 67 (MCA1614) used for the identification of CD55 expressing cells in rheumatoid arthritis synovial tissue by immunohistochemistry on cryostat sections.
Image caption:
Representative double staining of rheumatoid arthritis synovial tissue with antibodies against RasGRF1 and cell-specific markers. Synovial tissue sections were stained overnight with antibodies against Ras guanine nucleotide-releasing factor 1(RasGRF1), followed by antibodies against CD3, CD55, and CD68. After biotin tyramide enhancement, staining was developed with amino-ethylcarbazole (red, RasGRF1) and Fast blue (blue, cell-specific markers). Magnification x 100.

From: Abreu JR, de Launay D, Sanders ME, Grabiec AM, van de Sande MG, Tak PP, Reedquist KA.
The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue.
Arthritis Res Ther. 2009;11(4):R121.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD55 Antibody, clone 67 gallery image 9
Published customer image:
Mouse anti CD55 antibofdy, clone 67 (MCA1614) used for the demonstration of CD55 and MPGES1 in fibroblasts from sinovial lining by immunofluorescence.
Image caption:
MPGES1 expression in synovial lining fibroblasts before and 16 weeks after initiation of rituximab therapy.
Double immunofluorescence pictures show the presence of MPGES1 (red) expression in CD55 positive fibroblasts (green) in the rheumatoid tissue before rituximab initiation (A) and 16 weeks later (B). Original magnification 500x. Arrows point to double stained cells

From: Gheorghe KR, Thurlings RM, Westman M, Boumans MJ, Malmström V, Trollmo C, et al. (2011)
Prostaglandin E2 Synthesizing Enzymes in Rheumatoid Arthritis B Cells and the Effects of B Cell Depleting Therapy on Enzyme Expression.
PLoS ONE 6(1): e16378.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Human CD55:RPE

Product Type
Monoclonal Antibody
Clone
67
Isotype
IgG1
Specificity
CD55

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Mouse anti Human CD55 antibody, clone 67 recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types.

CD55 is the complement regulatory protein, decay accelerating factor (DAF) (Lublin and Atkinson 1989). Human CD55 is a ~70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens.

DAF has a wide distribution on cells in non-haematopoietic tissues, particularly epithelium and is found at the fetal-maternal interface in placenta (Holmes et al. 1990 and Yang et al. 2009). Soluble forms of DAF are found, for example, in plasma, saliva and urine (Medof et al. 1987). The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
K562 cells
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/2
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100&mul

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD55 antibody

  1. Hadam, M.R. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.
    Edited by Knapp, W. et al. Oxford University Press pp 694-697.
  2. Holmes, C.H. et al. (1990) Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.
    J Immunol. 144 (8): 3099-105.
  3. O'Brien, D.P. et al. (2009) Regulation of the Helicobacter pylori cellular receptor decay-accelerating factor.
    J Biol Chem. 283: 23922-30.
  4. Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
    Invest Ophthalmol Vis Sci. 50: 3473-81.
  5. van de Sande, M.G. et al. (2011) Different stages of rheumatoid arthritis: features of the synovium in the preclinical phase.
    Ann Rheum Dis. 70: 772-7.
  6. Mo, B. et al. (2006) ECC-1 cells: a well-differentiated steroid-responsive endometrial cell line with characteristics of luminal epithelium.
    Biol Reprod. 75: 387-94.
  7. Araten, D.J. et al. (2005) A quantitative measurement of the human somatic mutation rate.
    Cancer Res. 65: 8111-7.
  8. de Launay, D. et al. (2010) Silencing the expression of Ras family GTPase homologues decreases inflammation and joint destruction in experimental arthritis.
    Am J Pathol. 177: 3010-24.
    9. View The Latest Product References
  9. Gheorghe, K.R. et al. (2011) Prostaglandin E2 synthesizing enzymes in rheumatoid arthritis B cells and the effects of B cell depleting therapy on enzyme expression.
    PLoS One. ;6: e16378.
  10. Kraan, M.C. et al. (2004) T cells, fibroblast-like synoviocytes, and granzyme B+ cytotoxic cells are associated with joint damage in patients with recent onset rheumatoid arthritis.
    Ann Rheum Dis. 63: 483-8.
  11. van Holten, J. et al. (2005) A multicentre, randomised, double blind, placebo controlled phase II study of subcutaneous interferon beta-1a in the treatment of patients with active rheumatoid arthritis.
    Ann Rheum Dis. 64 (1): 64-9.
  12. Abreu, J.R. et al. (2009) The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue.
    Arthritis Res Ther.11(4):R121.
  13. Thurlings, R.M. et al. (2008) Synovial tissue response to rituximab: mechanism of action and identification of biomarkers of response.
    Ann Rheum Dis. 67 (7): 917-25.
  14. Vos, K. et al. (2007) Early effects of rituximab on the synovial cell infiltrate in patients with rheumatoid arthritis.
    Arthritis Rheum. 56 (3): 772-8.
  15. Edginton S et al. (2016) Effects of Rituximab and Infliximab Treatment on Carboxypeptidase B and Its Substrates in RA Synovium.
    J Rheumatol. 43 (5): 846-54.

Further Reading

  1. Lublin, D.M. & Atkinson, J.P. (1989) Decay-accelerating factor: biochemistry, molecular biology, and function.
    Annu Rev Immunol. 7: 35-58.
  2. Daniels, G. (1989) Cromer-related antigens--blood group determinants on decay-accelerating factor.
    Vox Sang. 56 (4): 205-11.

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
DAF
RRID
AB_321500
UniProt
P08174
Entrez Gene
CD55
GO Terms
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0005625 soluble fraction
GO:0006958 complement activation, classical pathway
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0031225 anchored to membrane
GO:0045087 innate immune response
GO:0045121 membrane raft
GO:0045730 respiratory burst

MCA1614PE

1114 1608

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