CD52 antibody | Campath-1H
0.5 μg of Human Anti-CD52 Antibody, MCA6101 (alemtuzumab biosimilar, research grade) and alemtuzumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-CD52 Antibody, MCA6101 (alemtuzumab biosimilar, research grade) and alemtuzumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 μl/min; inj. volume [μl]: 3; temp.: 25 °C; detection: 280 nm; sample: mAb (1 mg/ml).
A microtiter plate was coated overnight with Human Anti-Alemtuzumab Antibody (clone AbD19189, HCA198) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of alemtuzumab (therapeutic reference product) or Human Anti-CD52 Antibody, MCA6101 (alemtuzumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody (clone AbD16942_hIgG1, HCA175P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti CD52 (Alemtuzumab Biosimilar)
- Product Type
- Monoclonal Antibody
- Clone
- Campath-1H
- Isotype
- IgG1
- Specificity
- CD52
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| Human Anti-CD52 Antibody, clone Campath-1H is a research grade biosimilar of the monoclonal antibody drug alemtuzumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic antibody Campath-1H. Human Anti-CD52 Antibody is for research use only. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-alemtuzumab antibodies, for example HCA198 and HCA175. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-alemtuzumab antibody in IgG1 format as reference standard, for example HCA175. Alemtuzumab is a drug used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis. This product is NOT FOR THERAPEUTIC USE. View a summary of supporting anti-alemtuzumab antibody products |
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Proclin 300
- Immunogen
- Human lymphocytes
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Hispec Assay Diluent | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
- UniProt
- P31358
- Entrez Gene
- CD52
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005624 membrane fraction
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0031225 anchored to membrane
- GO:0045730 respiratory burst
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
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