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CD46 antibody | MEM-258

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Anti Human CD46 Antibody, clone MEM-258 thumbnail image 1 Anti Human CD46 Antibody, clone MEM-258 thumbnail image 2 Anti Human CD46 Antibody, clone MEM-258 thumbnail image 3 Anti Human CD46 Antibody, clone MEM-258 thumbnail image 4
Anti Human CD46 Antibody, clone MEM-258 gallery image 1
Figure A. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse anti Human CD46 (MCA2113PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Anti Human CD46 Antibody, clone MEM-258 gallery image 2
Published customer image:
Mouse anti Human CD46 antibody, clone MEM-258 (MCA2113) used for blocking adenovirus attachment to cells in vitro in blocking experiments on smoooth muscle and endothelial cells.
Image caption:
In vitro virus transduction. β-Gal activity and representative images of X-gal stained cells in A. HSVSMC and B. HSVEC infected with 5000 vp/cell for 3 hours at 37°C. β-Gal activity was normalised to total protein levels (* = p <0.05 compared to Ad5, *** = p <0.001 compared to Ad5). β-Gal activity normalised to total protein in (C) HSVSMC and (D) HSVEC infected with 5000 vp/cell in the presence or absence of CD46 function-blocking antibody MEM258 or an isotype matched control. (* = p <0.05 relative to isotype control, ** = p <0.01 relative to isotype control).

From: White KM, Alba R, Parker AL, Wright AF, Bradshaw AC, Delles C, McDonald RA,Baker AH.
Assessment of a novel, capsid-modified adenovirus with an improved vascular gene transfer profile.
J Cardiothorac Surg. 2013 Aug 9;8:183.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD46 Antibody, clone MEM-258 gallery image 3
Published customer image:
Muse ati HumanCD46 antibody, clone MEM-258 (MCA2113) used for the evaluation of CD46 expression on human cell lines by flow cytometry.
Image caption:
Detection of CD46, HSPG, CD80, and CD86 surface expression on HeLa, A549, K562, and Ramos cells. Overlays of representative flow cytometry charts are shown.

From: Tuve S, Wang H, Jacobs JD, Yumul RC, Smith DF, Lieber A (2008)
Role of Cellular Heparan Sulfate Proteoglycans in Infection of Human Adenovirus Serotype 3 and 35.
PLoS Pathog 4(10): e1000189.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD46 Antibody, clone MEM-258 gallery image 4
Published customer image:
FITC conjugated Mouse anti Human CD46 antibody, clone MEM-258 (MCA2113F) used for the evaluation of CD46 expression levels on human cells and porcine cells transfected with human CD46 by flow cytometry.
Image caption:
Expression of Gal and CD46 on PBMC (A) and aortic endothelial cells (B) from WT, GTKO, CD46 (homozygous), and GTKO/CD46 (heterozygous) pigs and humans. Cells were stained with BSI‐B4 (a), or CD46 (b). Representative data of three experiments. Dotted lines – unstained or isotype control. Black lines – WT cells. Red lines – GTKO cells. Green lines – CD46 (homozygous) cells. Blue lines – GTKO/CD46 (heterozygous) cells. Purple lines – human cells. Although WT and CD46 pigs expressed Gal on PBMC and PAEC, GTKO and GTKO/CD46 pigs and humans did not express Gal on PBMC or on aortic endothelial cells (A[a] and B[a]). CD46 and GTKO/CD46 pigs expressed higher levels of CD46 on PBMC and PAEC than WT or GTKO pigs (A[b] and B[b]). CD46 (homozygous) pig PBMC and PAEC expressed higher levels of CD46 than GTKO/CD46 (heterozygous) pig cells and human cells.

From: Hara H, Long C, Lin YJ, Tai HC, Ezzelarab M, Ayares D, Cooper DK.
In vitro investigation of pig cells for resistance to human antibody-mediated rejection.
Transpl Int. 2008 Dec;21(12):1163-74.
doi: 10.1111/j.1432-2277.2008.00736.x.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Human CD46:RPE

Product Type
Monoclonal Antibody
Clone
MEM-258
Isotype
IgG1
Specificity
CD46

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Product Code Applications Pack Size List Price Your Price Qty
MCA2113PET
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SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
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MCA2113PE
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SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Human CD46 antibody, clone MEM-258 recognizes the human CD46 cell surface antigen, also known as membrane co-factor protein (MCP), Trophoblast leukocyte common antigen or TLX. CD46 is a 392 amino acid (including a 34 aa signal peptide) ~43-60 kDa single pass type 1 transmembrane glysoprotein expressed by all cell types with the exception of erythrocytes.

CD46 functions as areceptor for complement and inhibitor of complement activation, limiting the formation and activity of C3 convertases. CD46 is expressed by all nucleated cells, often as multiple isoforms (Seya et al. 1993) on the same cells. The molecule is also expressed by sperm and may be important in the process of fertilisation (Carver-Ward et al. 1996).

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 25 Tests/0.25ml
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Pack Size: 100 Tests
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
HPB-ALL cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Store at +4°C.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 or 100μl whole blood

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
List Price Your Price
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD46 antibody

  1. Sirena D et al. (2004) The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3.
    J Virol. 78 (9): 4454-62.
  2. Fremeaux-Bacchi, V. et al. (2006) Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome.
    J Am Soc Nephrol. 17 (7): 2017-25.
  3. Fleischli, C. et al. (2005) The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35.
    J Virol. 79:10013-22.
  4. Sweigard, J.H. et al. (2010) Adenovirus vectors targeting distinct cell types in the retina.
    Invest Ophthalmol Vis Sci. 51:2219-28.
  5. Yang, P. et al. (2009) Expression and modulation of RPE cell membrane complement regulatory proteins.
    Invest Ophthalmol Vis Sci. 50: 3473-81.
  6. Bahat, A. and Eisenbach, M. (2010) Human sperm thermotaxis is mediated by phospholipase C and inositol trisphosphate receptor Ca2+ channel.
    Biol Reprod. 82: 606-16.
  7. Bienaime, F. et al. (2010) Mutations in components of complement influence the outcome of Factor I-associated atypical hemolytic uremic syndrome.
    Kidney Int. 77: 339-49.
  8. Wang, H. et al. (2008) In vitro and in vivo properties of adenovirus vectors with increased affinity to CD46.
    J Virol. 82: 10567-79.
    9. View The Latest Product References
  9. Hara, H. et al. (2011) Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
    Invest Ophthalmol Vis Sci. 52: 5278-86.
  10. El Karoui, K. et al. (2012) A clinicopathologic study of thrombotic microangiopathy in IgA nephropathy.
    J Am Soc Nephrol. 23 (1): 137-48.
  11. Bach, P. et al. (2013) Specific elimination of CD133+ tumor cells with targeted oncolytic measles virus.
    Cancer Res. 73 (2): 865-74.
  12. Leaderer, D. et al. (2015) Adeno-associated virus mediated delivery of an engineered protein that combines the complement inhibitory properties of CD46, CD55 and CD59.
    J Gene Med. 17 (6-7): 101-15.
  13. Tuve, S. et al. (2006) A new group B adenovirus receptor is expressed at high levels on human stem and tumor cells.
    J Virol. 80 (24): 12109-20.
  14. Hara, H. et al. (2008) In vitro investigation of pig cells for resistance to human antibody-mediated rejection.
    Transpl Int. 21 (12): 1163-74.
  15. Loré, K. et al. (2007) Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses.
    J Immunol. 179 (3): 1721-9.
  16. Fremeaux-Bacchi, V. et al. (2007) Unusual clinical severity of complement membrane cofactor protein-associated hemolytic-uremic syndrome and uniparental isodisomy.
    Am J Kidney Dis. 49 (2): 323-9.
  17. Le Quintrec, M. et al. (2008) Complement mutation-associated de novo thrombotic microangiopathy following kidney transplantation.
    Am J Transplant. 8 (8): 1694-701.
  18. Boyer, O. et al. (2008) Complement factor H deficiency and posttransplantation glomerulonephritis with isolated C3 deposits.
    Am J Kidney Dis. 51 (4): 671-7.
  19. Wang, H. et al. (2009) Receptor usage of a newly emergent adenovirus type 14.
    Virology. 387 (2): 436-41.
  20. Iguchi, K. et al. (2012) Efficient antitumor effects of carrier cells loaded with a fiber-substituted conditionally replicating adenovirus on CAR-negative tumor cells.
    Cancer Gene Ther. 19 (2): 118-25.
  21. Kälin, S. et al. (2010) Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35.
    J Virol. 84 (10): 5336-50.
  22. Bottino, R. et al. (2014) Pig-to-monkey islet xenotransplantation using multi-transgenic pigs.
    Am J Transplant. 14 (10): 2275-87.
  23. White, K.M. et al. (2013) Assessment of a novel, capsid-modified adenovirus with an improved vascular gene transfer profile.
    J Cardiothorac Surg. 8: 183.
  24. Tuve, S. et al. (2008) Role of cellular heparan sulfate proteoglycans in infection of human adenovirus serotype 3 and 35.
    PLoS Pathog. 4 (10): e1000189.
  25. Iwase, H. et al. (2014) Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition.
    Xenotransplantation. 21 (1): 72-83.
  26. Sweigard, J.H. et al. (2011) Adenovirus-mediated delivery of CD46 attenuates the alternative complement pathway on RPE: implications for age-related macular degeneration.
    Gene Ther. 18 (6): 613-21.

Flow Cytometry

Functional Assays

Immunofluorescence

Synonyms
Membrane CO-FACTOR Protein
RRID
AB_324204
UniProt
P15529
Entrez Gene
CD46

MCA2113PET

159000 1701

MCA2113PE

1113 1701

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