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CD45RA antibody | F8-11-13

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Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 1 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 2 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 3 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 4 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 5 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 6 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 7 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 8 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 9 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 10 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 11 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 12 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 13 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 14 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 15 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 16 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 17 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 18 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 19 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 20 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 21 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 22 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 23 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 24 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 25 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 26 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 27 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 28 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 29 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 30 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 31 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 32 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 33 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 34 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 35 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 36 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 37 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 38 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 39 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 40 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 41 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 42 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 43 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 44 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 45 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 46 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 47 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 48 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 49 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 50 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 51 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 52 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 53 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 54 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 55 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 56 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 57 Anti Human CD45RA Antibody, clone F8-11-13 thumbnail image 58
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 1
Figure A. PE conjugated Mouse anti Human CD45RO (MCA461PE) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. PE conjugated Mouse anti Human CD3 (MCA461PE) and Amethyst Orange conjugated Mouse anti Human CD45RA (MCA88AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 2
Figure A. PE-Cy5 conjugated mouse anti human CD45RO (MCA461C) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE-Cy5 conjugated mouse anti human CD45RO (MCA461C) and FITC conjugated mouse anti human CD45RA (MCA88F). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A)
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 3
Figure A. RPE conjugated mouse anti human CD45RO (MCA461PE) and PE-A647 conjugated mouse IgG1 isotype control (MCA928P647). Figure B. RPE conjugated mouse anti human CD45RO (MCA461PE) and PE-A647 conjugated mouse anti Human CD45RA (MCA88P647). All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 4
Figure A. RPE conjugated mouse anti human CD45RO (MCA461PE) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. RPE conjugated mouse anti human CD45RO (MCA461PE) and A647 conjugated mouse anti Human CD45RA (MCA88A647). All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 5
Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human CD45RA (MCA88).
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 6
Published customer image:
AlexaFluor 700 conjugated Mouse anti Human CD45RA antibody (MCA88A700) used for the phenotypic characterization of CD8 expressing T cells by flow cytometry.
Image caption:
CD8 T cell subpopulation distribution in the blood and endocervix.
(A) A diagram of Sallusto and Lanzavecchia’s classification scheme of CD8 T cells based on CD45RA and CCR7 expression and relative perforin expression of the different subpopulation, (B) distribution of the different CD8 T cell subpopulations in a representative C. trachomatis-negative and C. trachomatis-positive women (C-D) Distribution of different CD8 T cells in the blood (C), and in the endocervix (D) of 16 women (n=16). Using flow cytometric analyses, CD8 T cells were identified in a population of cells from PBMC and endocervical cells by gating for CD3+CD8+ cells. CD8 T cells were further characterized based on CD45RA and CCR7 expression. Naïve cells are CD45RA+ CCR7+, central memory (TCM) cells are CD45RA-CCR7+, effector memory (TEM) cells are CD45RA- CCR7-, and terminally differentiated effector memory (TEMRA) cells are CD45RA+ CCR7-. The majority of CD8 T cells in the endocervix are TEM CD8 subtype.

From: Ibana JA, Myers L, Porretta C, Lewis M, Taylor SN, Martin DH, Quayle AJ.
The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.
BMC Immunol. 2012 Dec 7;13:66.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 7
Figure A. PE conjugated Mouse anti Human CD45RO (MCA461PE). Figure B. PE conjugated Mouse anti Human CD45RO (MCA461PE) and SBV515 conjugated Mouse anti Human CD45RA (MCA88SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 8
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and SBV515 conjugated Mouse anti Human CD45RO (MCA461SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 9
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright Violet 670 conjugated Mouse anti Human CD45RO (MCA461SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 10
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RO (MCA461SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RO (MCA461SBV440) and StarBright Violet 670 conjugated Mouse anti Human CD45RA (MCA88SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 11
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RO (MCA461SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RO (MCA461SBV610) and StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 12
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700) and StarBright Violet 440 conjugated Mouse anti Human CD45RO (MCA461SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 13
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RO (MCA461SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RO (MCA461SBB700) and StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 14
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright Violet 610 conjugated Mouse anti Human CD45RO (MCA461SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 15
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RO (MCA461SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RO (MCA461SBB700) and StarBright Violet 570 conjugated Mouse anti Human CD45RA (MCA88SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 16
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright Violet 570 conjugated Mouse anti Human CD45 (MCA461SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 17
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RO (MCA461SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RO (MCA461SBV440) and StarBright Violet 710 conjugated Mouse anti Human CD45RA (MCA88SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 18
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515) and StarBright Violet 710 conjugated Mouse anti Human CD45RO (MCA461SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 19
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610) and StarBright Violet 790 conjugated Mouse anti Human CD45RO (MCA461SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 20
Figure A. PE conjugated Mouse anti Human CD45RO (MCA461PE). Figure B. PE conjugated Mouse anti Human CD45RO (MCA461PE) and StarBright Violet 790 conjugated Mouse anti Human CD45RA (MCA88SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 21
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD45RO (MCA461SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD45RO (MCA461SBV710) and StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA88SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 22
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700) and StarBright Violet 475 conjugated Mouse anti Human CD45RO (MCA461SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 23
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700) and StarBright UltraViolet 400 conjugated Mouse anti Human CD45R) (MCA461SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 24
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RO (MCA461SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RO (MCA461SBV610) and StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 25
Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (MCA461SBUV510). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (MCA461SBUV510) and StarBright Violet 760 conjugated Mouse anti Human CD45RA (MCA88SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 26
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400) and StarBright Violet 760 conjugated Mouse anti Human CD45 (MCA461SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 27
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD45RO (MCA461SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD45RO (MCA461SBV475) and StarBright UltraViolet 665 conjugated Mouse anti Human CD45RA (MCA88SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 28
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright UltraViolet 665 conjugated Mouse anti Human CD45RO (MCA461SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 29
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610) and StarBright UltraViolet 445 conjugated Mouse anti Human CD45RO (MCA461SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 30
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD45RO (MCA461SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD45RO (MCA461SBV710) and StarBright UltraViolet 445 conjugated Mouse anti Human CD45RA (MCA88SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 31
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright UltraViolet 795 conjugated Mouse anti human CD45RO (MCA461SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 32
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA461SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA461SBV475) and StarBright UltraViolet 795 conjugated Mouse anti human CD45RA (MCA88SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 33
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD45RO (MCA461SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD45RO (MCA461SBV790) and StarBright UltraViolet 510 conjugated Mouse anti Human CD45RA (MCA88SBUV510). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 34
Figure A. StarBright Ultra Violet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795). Figure B. StarBright Ultra Violet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795) and StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (MCA461SBUV510). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 35
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RO (MCA461SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RO (MCA461SBB700) and StarBright UltraViolet 575 conjugated Mouse anti Human CD45RA (MCA88SBUV575). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 36
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD45RA (MCA88A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD45RA (MCA88A700) and StarBright UltraViolet 575 conjugated Mouse anti Human CD45RO (MCA461SBUV575). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 37
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD45RO (MCA461SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD45RO (MCA461SBV515) and StarBright UltraViolet 605 conjugated Mouse anti Human CD45RA (MCA88SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 38
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright UltraViolet 605 conjugated Mouse anti Human CD45 (MCA461SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 39
Figure A. Starbright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. Starbright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright Blue 580 conjugated Mouse anti Human CD45RO (MCA461SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 40
Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (MCA461SBUV510). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (MCA461SBUV510) and StarBright Blue 580 conjugated Mouse anti Human CD45RA (MCA88SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 41
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RO (MCA461SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RO (MCA461SBUV795) and StarBright Blue 615 conjugated Mouse anti Human CD45RA (MCA88SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 42
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795) and StarBright Blue 615 conjugated Mouse anti Human CD45RO (MCA461SBB615). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 43
Figure A. RPE conjugated Mouse anti Human CD45RO (MCA461PE). Figure B. RPE conjugated Mouse anti Human CD45RO (MCA461PE) and StarBright Blue 675 conjugated Mouse anti Human CD45RA (MCA88SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 44
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400) and StarBright Blue 675 conjugated Mouse anti Human CD45RA (MCA461SBB675). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 45
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright Blue 765 conjugated Mouse anti Human CD45RO (MCA461SBB765). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 46
Figure A. StarBright UltraViolet 605 conjugated Mouse anti Human CD45RO (MCA461SBUV605). Figure B. StarBright UltraViolet 605 conjugated Mouse anti Human CD45RO (MCA461SBUV605) and StarBright Blue 765 conjugated Mouse anti Human CD45RA (MCA88SBB765). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 47
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515) and StarBright Blue 810 conjugated Mouse anti Human CD45RO (MCA461SBB810). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 48
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RO (MCA461SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RO (MCA461SBV440) and StarBright Blue 810 conjugated Mouse anti Human CD45RA (MCA88SBB810). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 49
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD45RO (MCA461SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD45RO (MCA461SBV710) and StarBright Yellow 575 conjugated Mouse anti Human CD45RA (MCA88SBY575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 50
Figure A. StarBright Violet 760 conjugated Mouse anti Human CD45RA (MCA88SBV760). Figure B. StarBright Violet 760 conjugated Mouse anti Human CD45RA (MCA88SBV760) and StarBright Yellow 575 conjugated Mouse anti Human CD45RO (MCA461SBY575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 51
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RO (MCA461SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RO (MCA461SBUV400) and StarBright Yellow 720 conjugated Mouse anti Human CD45RA (MCA88SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 52
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA88SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA88SBV475) and StarBright Yellow 720 conjugated Mouse anti Human CD45RO (MCA461SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 53
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD45RO (MCA461SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD45RO (MCA461SBB765) and StarBright Yellow 605 conjugated Mouse anti Human CD45RA (MCA88SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 54
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright Yellow 605 conjugated Mouse anti Human CD45RO (MCA461SBY605). All experiments performed on human PBMCs stimulated with PHA for 3 days gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 55
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD45A (MCA88SBV570). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD45A (MCA88SBV570) and StarBright Yellow 665 conjugated Mouse anti Human CD45RA (MCA461SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 56
Figure A. StarBright Violet 570 conjugated Mouse anti Human CD45RO (MCA461SBV570). Figure B. StarBright Violet 570 conjugated Mouse anti Human CD45RO (MCA461SBV570) and StarBright Yellow 665 conjugated Mouse anti Human CD45RA (MCA88SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 57
Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD45RO (MCA461SBY575). Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD45RO (MCA461SBY575) and StarBright Yellow 800 conjugated Mouse anti Human CD45RA (MCA88SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD45RA Antibody, clone F8-11-13 gallery image 58
Figure A. StarBright Blue 675 conjugated Mouse anti Human CD45RA (MCA88SBB675). Figure B. StarBright Blue 675 conjugated Mouse anti Human CD45RA (MCA88SBB675) and StarBright Yellow 800 conjugated Mouse anti Human CD45RO (MCA461SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD45RA:Alexa Fluor® 700

Product Type
Monoclonal Antibody
Clone
F8-11-13
Isotype
IgG1
Specificity
CD45RA

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MCA88A700
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Mouse anti Human CD45RA antibody, clone F8-11-13 recognizes the human CD45RA cell surface antigen.

CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.

Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human T lymphocytes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mouse were fused with cells of the NS-1 mouse myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®700 702 723
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul lysed whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 700 MCA928A700 F 100 Tests/1ml loader
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Description Mouse IgG1 Negative Control:Alexa Fluor® 700

Description Product Code Applications Pack Size List Price Your Price Quantity
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References for CD45RA antibody

  1. Dalchau, R. & Fabre, J.W. (1981) Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule.
    J Exp Med. 153 (4): 753-65.
  2. O'Grady, J.T. et al. (1994) CD40 expression in Hodgkin's disease.
    Am J Pathol. 144 (1): 21-6.
  3. Franitza, S. et al. (2002) TGF-beta1 enhances SDF-1alpha-induced chemotaxis and homing of naive T cells by up-regulating CXCR4 expression and downstream cytoskeletal effector molecules.
    Eur J Immunol. 32 (1): 193-202.
  4. Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of human immunodeficiency virus-infected persons with oropharyngeal Candidiasis.
    Clin Vaccine Immunol. 13 (6): 678-83.
  5. Hunt, L. et al. (2016) T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals.
    Ann Rheum Dis. 75 (10): 1884-9.
  6. Ibana, J.A. et al. (2012) The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.
    BMC Immunol. 13: 66.
  7. Ponchel, F. et al. (2014) An immunological biomarker to predict MTX response in early RA.
    Ann Rheum Dis. 73 (11): 2047-53.

Immunohistology - Frozen

UniProt
P08575
Entrez Gene
PTPRC
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus

MCA88A700

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