CD38 antibody | AT13/5
Mouse anti Human CD38 antibody, clone AT13/5 (MCA1019PE) used to evaluate CD38 expression on peripheral blood mononuclear cells by flow cytometry.
Image caption:
CD38brightHLA-CD69+ NK cells. Representative flow cytometry plots from NK cell subsets showing identification of CD38brightCD69+ cells in (A) CD56bright and (B) CD56dim subsets at 1 and 6 months of age an HIV-infected infant (B1-093). (C) Plots show individual infant trajectories as connected lines of different colors, with overlaid linear mixed models (LMM). LMM were used to determine whether slope (change in % of cells/month) was affected by HIV infection status; where slopes are not different, the slope representing the combined data of all infants is shown to the box on the right. The mean difference across HIV groups is derived from the beta coefficient for [HIV status] as described in the Materials and Methods, and represents the % increase in cells per HIV category from HIV-unexposed to HIV-exposed uninfected to HIV-infected. Slopes for each HIV infection group are also presented separately within each graph.
From: Slyker JA, Lohman-Payne B, John-Stewart GC, Dong T, Mbori-Ngacha D, Tapia K, Atzberger A, Taylor S, Rowland-Jones SL, Blish CA.
The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 2012 Dec 31;3:399.
doi: 10.3389/fimmu.2012.00399.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD38:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- AT13/5
- Isotype
- IgG1
- Specificity
- CD38
Antibody Quality and Performance Guarantee
| Mouse anti Human CD38 antibody, clone AT13/5 recognizes CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1 or T10. CD38 is a 300 amino acid ~45 kDa single pass type II transmembrane glycoprotein and Mouse anti Human CD38 antibody, clone AT13/5 has a reactivity pattern consistent with other antibodies of the CD38 cluster. CD38 is expressed by plasma cells, monocytes, early lymphoid cells and activated T cells. CD38 is widely used to study the processes of B and T differentiation and activation. CD38 is a multifunctional enzyme involved in calcium signaling and nicotinamide adenine dinucleotide (NAD+) metabolism (de Oliveira et al. 2018). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor 488- liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Namalwa human B-cell line.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 488 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
Source Reference
-
Ellis, J.H. et al. (1995) Engineered anti-CD38 monoclonal antibodies for immunotherapy of multiple myeloma.
J Immunol. 155 (2): 925-37.
References for CD38 antibody
-
Corcione, A. et al. (2009) CX3CR1 is expressed by human B lymphocytes and mediates [corrected] CX3CL1 driven chemotaxis of tonsil centrocytes.
PLoS One. 4: e8485. -
Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12: 2. -
Tipping, A.J. et al. (2009) High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle
Blood. 113: 2661-72. -
Slyker, J.A. et al. (2012) Acute cytomegalovirus infection is associated with increased frequencies of activated and apoptosis-vulnerable T cells in HIV-1-infected infants.
J Virol. 86 (20): 11373-9. -
Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 3: 399. -
Kelleher, M. et al. (2011) Comparative Kinetics of Immune Responses and Changes in Cellular Sub-Sets Detected in Colorectal Cancer Patients Vaccinated with MVA-5T4 (TroVax) Administered Alongside Two Different Chemotherapy Regimens
J Cancer Therapy. 02 (01): 54-64.
Further Reading
-
Malavasi, F. et al. (1994) Human CD38: a glycoprotein in search of a function.
Immunol Today. 15 (3): 95-7.
- RRID
- AB_321678
- UniProt
- P28907
- Entrez Gene
- CD38
- GO Terms
- GO:0005886 plasma membrane
- GO:0003953 NAD+ nucleosidase activity
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005488 binding
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0042493 response to drug
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- View More GO Terms
- GO:0030890 positive regulation of B cell proliferation
- GO:0043066 negative regulation of apoptosis
- GO:0050853 B cell receptor signaling pathway
MCA1019A488T
151564
MCA1019A488
0215
166475
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