CD32 antibody | AT10
Mouse anti Human CD32:RPE
- Product Type
- Monoclonal Antibody
- Clone
- AT10
- Isotype
- IgG1
- Specificity
- CD32
Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Rhesus Monkey Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- MCA1075PET: Reconstitute with 0.25ml distilled water
- MCA1075PE: Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
-
MCA1075PET: 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose -
MCA1075PE: 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- K562 cell line.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
Source Reference
-
Greenman, J. et al. (1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.
Mol Immunol. 28 (11): 1243-54.
References for CD32 antibody
-
Van Den Herik-Oudijk, I.E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.
J Immunol. 152 (2): 574-85. -
Larsson M et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro.
Immunology. 90 (1): 138-46. -
Lilliehöök, I. et al. (1998) Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.
Vet Immunol Immunopathol. 61 (2-4): 181-93. -
Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.
J Immunol. 163: 1409-19. -
Su, K. et al. (2004) A promoter haplotype of the immunoreceptor tyrosine-based inhibitory motif-bearing FcgammaRIIb alters receptor expression and associates with autoimmunity. II. Differential binding of GATA4 and Yin-Yang1 transcription factors and correlated receptor expression and function.
J Immunol. 172: 7192-9. -
Pawar, P. et al. (2004) Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets.
J Immunol. 173: 1258-65. -
Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.
Blood. 105: 4390-8. -
Bonnefont-Rebeix, C. et al. (2006) CD86 molecule is a specific marker for canine monocyte-derived dendritic cells.
Vet Immunol Immunopathol. 109 (1-2): 167-76.
View The Latest Product References
-
Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
J Exp Med. 203: 1629-35. -
Mold, C. and Du Clos, T.W. (2006) C-reactive protein increases cytokine responses to Streptococcus pneumoniae through interactions with Fc gamma receptors.
J Immunol. 176: 7598-604. -
Dutertre, C.A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
J Leukoc Biol. 84 (6): 1511-20. -
Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149. -
Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.
Infect Immun. 78: 4268-75. -
Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
J Immunol. 185: 4738-49. -
Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
Vet Immunol Immunopathol. 135: 188-98. -
Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. -
Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
Mol Immunol. 49 (1-2): 211-8. -
Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.
Proc Natl Acad Sci U S A. 108 (45): 18342-7. -
van der Heijden, J. et al. (2012) Phenotypic variation in IgG receptors by nonclassical FCGR2C alleles.
J Immunol. 188 (3): 1318-24. -
Pellerin, A. et al. (2015) Anti-BDCA2 monoclonal antibody inhibits plasmacytoid dendritic cell activation through Fc-dependent and Fc-independent mechanisms.
EMBO Mol Med. 7 (4): 464-76. -
Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Vet Parasitol. 220: 33-45. -
Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans by granulocytes mobilized for transfusion purposes: a role for granule components.
Haematologica. 101 (5): 587-96. -
Petersson, F. et al. (2018) Platelet activation and aggregation by the opportunistic pathogen Cutibacterium (Propionibacterium) acnes.
PLoS One. 13 (1): e0192051. -
Bruggeman, C.W. et al. (2019) Tissue-specific expression of IgG receptors by human macrophages ex vivo.
PLoS One. 14 (10): e0223264. -
Chen, T. et al. (2020) Capsular glycan recognition provides antibody-mediated immunity against tuberculosis.
J Clin Invest. 130 (4): 1808-22. -
Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print]. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16): 2103. -
Jimenez, R.V. et al. (2018) C-Reactive Protein Impairs Dendritic Cell Development, Maturation, and Function: Implications for Peripheral Tolerance.
Front Immunol. 9: 372.
- Synonyms
- FcRII
- RRID
- AB_321661
- UniProt
- P12318
- P31994
- P31995
- Entrez Gene
- FCGR2A
- FCGR2B
- FCGR2C
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0019864 IgG binding
- GO:0006955 immune response
- GO:0044419 interspecies interaction between organisms
- GO:0050776 regulation of immune response
- GO:0004888 transmembrane receptor activity
- GO:0005737 cytoplasm
- View More GO Terms
MCA1075PET
1610MCA1075PE
151675 1603If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up