CD30 antibody | cAC10

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Human anti CD30 (Brentuximab Biosimilar)

Anti-CD30 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug brentuximab for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.

Product Type
Monoclonal Antibody
Clone
cAC10
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6149
Datasheet
E
SDS
0.2 mg loader

Human Anti-CD30 antibody, clone cAC10, is a research grade biosimilar of the chimeric monoclonal antibody drug brentuximab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic brentuximab antibody clone cAC10. This product is supplied in a research grade format, i.e. in PBS buffer with preservative; it is not manufactured or formulated in the same way as the therapeutic reference product; it is not coupled to the drug monomethyl auristatin E (MMAE).

Brentuximab vedotin (Adcetris) is an antibody drug conjugate that combines the anti-CD30 antibody (brentuximab) with the anti-mitotic agent MMAE (vedotin). It is approved for the treatment of Hodgkin's lymphoma and systemic anaplastic large cell lymphoma. The brentuximab antibody selectively targets tumor cells expressing the cell membrane protein CD30 and delivers the MMAE, which causes apoptosis by preventing cell cycle progression of the G2 to M phase through disruption of the cytosolic microtuble network, thereby preventing tumor growth and proliferation.

This product is NOT FOR THERAPEUTIC USE.

Product Details

Target Species
Human
Product Form
Human IgG1 (kappa) antibody - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Human CD30
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P28908
Entrez Gene
TNFRSF8
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005737 cytoplasm
GO:0008285 negative regulation of cell proliferation
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0043065 positive regulation of apoptosis
GO:0045556 positive regulation of TRAIL biosynthetic process
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Licensed Use
For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Regulatory
For research purposes only

Applications of CD30 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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