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CD3 antibody | OKT3

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Anti Human CD3 Antibody, clone OKT3 thumbnail image 1 Anti Human CD3 Antibody, clone OKT3 thumbnail image 2 Anti Human CD3 Antibody, clone OKT3 thumbnail image 3 Anti Human CD3 Antibody, clone OKT3 thumbnail image 4 Anti Human CD3 Antibody, clone OKT3 thumbnail image 5 Anti Human CD3 Antibody, clone OKT3 thumbnail image 6 Anti Human CD3 Antibody, clone OKT3 thumbnail image 7 Anti Human CD3 Antibody, clone OKT3 thumbnail image 8 Anti Human CD3 Antibody, clone OKT3 thumbnail image 9 Anti Human CD3 Antibody, clone OKT3 thumbnail image 10 Anti Human CD3 Antibody, clone OKT3 thumbnail image 11 Anti Human CD3 Antibody, clone OKT3 thumbnail image 12 Anti Human CD3 Antibody, clone OKT3 thumbnail image 13 Anti Human CD3 Antibody, clone OKT3 thumbnail image 14
Anti Human CD3 Antibody, clone OKT3 gallery image 1
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and APC conjugated Mouse IgG2a isotype control (MCA929APC). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and APC conjugated Mouse anti Human CD3 (MCA6146APC). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 2
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD4 (MCA1267SBV790) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD4 (MCA1267SBV790) and FITC conjugated Mouse anti Human CD3 (MCA6146F). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 3
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and PE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and PE conjugated Mouse anti Human CD3 (MCA6146PE). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 4
Figure A. StarBright Blue 580 conjugated Mouse anti Human CD4 (MCA1267SBB580) and Alexa Fluor 700 conjugated Mouse IgG2a isotype control (MCA929A700). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD4 (MCA1267SBB580) and Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA6146A700). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 5
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV710) and Pacific Blue conjugated Mouse IgG2a isotype control (MCA929PB). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV710) and Pacific Blue conjugated Mouse anti Human CD3 (MCA6146PB). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 6
Figure A. StarBright Violet 570 conjugated Mouse anti Human CD4 MCA1267SBV570 and Alexa Fluor 488 conjugated Mouse IgG2a isotype control MCA929A488. Figure B. StarBright Violet 570 conjugated Mouse anti Human CD4 MCA1267SBV570 and Alexa Fluor 488 conjugated Mouse anti Human CD3 MCA6146A488. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 7
Figure A. StarBright Blue 810 conjugated Mouse anti Human CD4 MCA1267SBB810 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control MCA929A647. Figure B. StarBright Blue 810 conjugated Mouse anti Human CD4 MCA1267SBB810 and Alexa Fluor 647 conjugated Mouse anti Human CD3 MCA6146A647. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 8
Figure A. RPE conjugated Mouse anti Human CD3 (MCA6146PE). Figure B. RPE conjugated Mouse anti Human CD3 (MCA6146PE) and StarBright Blue 580 conjugated Mouse anti Human HLA ABC (MCA81SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 9
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA6146SBUV795). Figure B StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA6146SBUV795) and StarBright Blue 580 conjugated Mouse anti Human CD27 (MCA755SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 10
Figure A. APC conjugated Mouse anti Human CD3 (MCA6146APC). Figure B. APC conjugated Mouse anti Human CD3 (MCA6146APC) and StarBright Blue 580 conjugated Mouse anti Human CD45 (MCA87SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 11
Figure A. APC conjugated Mouse anti Human CD3 (MCA6146APC). Figure B. APC conjugated Mouse anti Human CD3 (MCA6146APC) and StarBright Blue 580 conjugated Mouse anti Human CD25 (MCA2127SBB580). All experiments performed on human PBMCs stimulated with PHA for 3 days, gated on live cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 12
Figure A. APC conjugated Mouse anti Human CD3 (MCA6146APC). Figure B. APC conjugated Mouse anti Human CD3 (MCA6146APC) and StarBright Blue 615 conjugated Mouse anti Human CD27 (MCA755SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 13
Figure A. RPE conjugated Mouse anti Human CD3 (MCA6146PE). Figure B. RPE conjugated Mouse anti Human CD3 (MCA6146PE) and StarBright Blue 675 conjugated Mouse anti Human CD27 (MCA755SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD3 Antibody, clone OKT3 gallery image 14
Figure A. APC conjugated Mouse anti Human CD3 (MCA6146APC). Figure B. APC conjugated Mouse anti Human CD3 (MCA6146APC) and StarBright Blue 765 conjugated Mouse anti Human HLA ABC (MCA81SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD3:StarBright Violet 570

Product Type
Monoclonal Antibody
Clone
OKT3
Isotype
IgG2a
Specificity
CD3

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F 100 Tests/0.5ml loader
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Mouse anti Human CD3 antibody, clone OKT3, recognizes T-cell surface glycoprotein CD3 epsilon chain (CD3ε), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3ε is a subunit within the multiprotein cell surface receptor TCR-CD3 complex, which is expressed on the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009).

Mice that lack the CD3ε gene exhibit early arrest in T cell development and mutation of the gene in humans has been seen to result in severe combined immunodeficiency (SCID) (DeJarnette et al. 1998, de Saint Basile et al. 2004).

Mouse anti Human CD3, clone OKT3 is commonly used in flow cytometry in the phenotyping human T cells. Characterization of the OKT3 clone has shown that it is able to compete and block the binding of the CD3 clones SK7 and UCHT1 to human T cells (Li et al. 2005). The OKT3 clone has also been found to have immunosuppressive properties in vivo. As result of this, the clone was humanized to be used as a therapeutic drug called Muromonomab (Orthoclone). Muromonomab was used as an immunosuppressive including for after solid organ transplants, to prevent the activation of CD4- and CD8- T cells (Wilde & Goa 1996). However, the drug has since been discontinued due to the development of other treatments with similar efficacy and fewer side effects (Reichert 2012).

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Violet 570 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Human peripheral blood lymphocytes
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 570 404 571
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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UniProt
P07766
Entrez Gene
CD3E
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway

MCA6146SBV570

100008298 100008299

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