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CD1a antibody | NA1/34-HLK

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SDS Safety Datasheet SDS
F 0.1 mg loader
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Mouse anti Human CD1a antibody, clone NA1/34-HLK recognizes the human CD1a cell surface glycoprotein, a ~49 kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like domain, expressed in association with beta 2 microglobulin. CD1a is expressed strongly by cortical thymocytes, and also by Langerhans cells and interdigitating cells. CD1a is involved in the presentation of lipids and glycolipids to NK cells (Sloma et al. 2008).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Human thymocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1/1 Ag4.1 mouse myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
List Price Your Price
Description Mouse IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. McMichael, A.J. et al. (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.
    Eur J Immunol. 9 (3): 205-10.

References for CD1a antibody

  1. Poulter, L.W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies.
    Scand J Immunol. 24 (3): 351-7.
  2. Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
    J Immunol. 161: 3966-73.
  3. Murray, S. et al. (2000) Diagnostic and therapeutic evaluation of an anti-Langerhans cell histiocytosis monoclonal antibody (NA1/34) in a new xenograft model.
    J Invest Dermatol. 114: 127-34.
  4. Buettner, M. et al. (2005) Inverse correlation of maturity and antibacterial activity in human dendritic cells.
    J Immunol. 174: 4203-9.
  5. Cox, K. et al. (2005) Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
    J Leukoc Biol. 78: 1142-52.
  6. Angel, C.E. et al. (2006) Distinctive localization of antigen-presenting cells in human lymph nodes.
    Blood. 113: 1257-67.
  7. Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation.
    Can J Vet Res. 71: 165-74.
  8. Angel, C.E. et al. (2007) Comprehensive analysis of MHC-II expression in healthy human skin.
    Immunol Cell Biol. 85: 363-9.
  9. View The Latest Product References
  10. Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
    Int Immunol. 19: 1271-9.
  11. Elia, A.R. et al. (2008) Human dendritic cells differentiated in hypoxia down-modulate antigen uptake and change their chemokine expression profile.
    J Leukoc Biol. 84: 1472-82.
  12. Liu, C.C. et al (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
    Cancer Immunol Immunother. 57: 479-91.
  13. Fanales-Belasio, E. et al. (2009) HIV-1 Tat addresses dendritic cells to induce a predominant Th1-type adaptive immune response that appears prevalent in the asymptomatic stage of infection.
    J Immunol. 182: 2888-97.
  14. Sugiura K et al. (2010) Effect of IL-12 on canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4.
    Vet Immunol Immunopathol. 137 (3-4): 322-6.
  15. Mito, K. et al. (2010) IFN{gamma} markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.
    Cancer Res. 70: 7093-101.
  16. Hirbod, T. et al. (2010) Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men.
    Am J Pathol. 176: 2798-805.
  17. Kaldensjö, T. et al. (2011) Detection of intraepithelial and stromal Langerin and CCR5 positive cells in the human endometrium: potential targets for HIV infection.
    PLoS One. 6: e21344.
  18. Bosco, M.C. et al. (2011) Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
    Blood. 117: 2625-39.
  19. Baharom, F. et al. (2016) Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung Mucosa of Healthy Humans.
    J Immunol. 196 (11): 4498-509.
  20. Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
    Immunobiology. 221 (1): 12-22.
  21. Zegarska, B. et al. (2017) Changes of Langerhans cells during skin ageing.
    Postepy Dermatol Alergol. 34 (3): 260-7.
  22. Tomić, S. et al. (2018) Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
    Int J Nanomedicine. 13: 6941-60.
  23. Guilliams, M. et al. (2016) Unsupervised High-Dimensional Analysis Aligns Dendritic Cells across Tissues and Species.
    Immunity. 45 (3): 669-84.

Flow Cytometry


Entrez Gene
GO Terms
GO:0042612 MHC class I protein complex
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0019882 antigen processing and presentation


151136 165331 1708

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