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CD19 antibody | Bu12

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Anti Human CD19 Antibody, clone Bu12 thumbnail image 1 Anti Human CD19 Antibody, clone Bu12 thumbnail image 2 Anti Human CD19 Antibody, clone Bu12 thumbnail image 3 Anti Human CD19 Antibody, clone Bu12 thumbnail image 4
Anti Human CD19 Antibody, clone Bu12 gallery image 1
Figure A. PE conjugated Mouse anti Human CD20 (MCA1710PE) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. PE conjugated Mouse anti Human CD20 (MCA1710PE) and Amethyst Orange conjugated Mouse anti Human CD19 (MCA2495AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD19 Antibody, clone Bu12 gallery image 2
Figure A. A647 conjugated Mouse anti Human CD20 (MCA1710A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. A647 conjugated Mouse anti Human CD20 (MCA1710A647) and FITC conjugated Mouse anti Human CD19 (MCA2495F). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Anti Human CD19 Antibody, clone Bu12 gallery image 3
Figure A. Alexa Fluor 488 conjugated mouse anti human CD20 (MCA1710A488) and Alexa Fluor647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. Alexa Fluor488 conjugated mouse anti human CD20 (MCA1710A488) and Alexa Fluor647 conjugated mouse anti human CD19 (MCA2495A647). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Anti Human CD19 Antibody, clone Bu12 gallery image 4
Figure A. Alexa Fluor 700 conjugated mouse anti human CD20 (MCA1710A700) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor700 conjugated mouse anti human CD20 (MCA1710A700) and RPE conjugated mouse anti human CD19 (MCA2495PE). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.

Mouse anti Human CD19:Amethyst Orange

Product Type
Monoclonal Antibody
Clone
Bu12
Isotype
IgG1
Specificity
CD19

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Product Code Applications Pack Size List Price Your Price Qty
MCA2495AMO
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD19 antibody, clone Bu12 recognizes human CD19, a 95 kDa cell surface glycoprotein, which is expressed on cells of the B cell lineage and follicular dendritic cells but absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.

Target Species
Human
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human EB-4 Burkitt lymphoma cell line
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells from the X63 AG8 653 plasmacytoma
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Amethyst Orange 405 540
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD19 antibody

  1. Smith SH et al. (1991) Activation of human B cells through the CD19 surface antigen results in homotypic adhesion by LFA-1-dependent and -independent mechanisms.
    Immunology. 73 (3): 293-7.
  2. Callard, R.E. et al. (1992) CD19 regulation of human B cell responses. B cell proliferation and antibody secretion are inhibited or enhanced by ligation of the CD19 surface glycoprotein depending on the stimulating signal used.
    J Immunol. 148 (10): 2983-7.
  3. Zhou, L. and Tedder, T. (1993) CD19 Workshop panel report.
    In leucocyte Typing V. White cell differentiation antigens. Oxford University press. p507 – 509.
  4. Callard, R.E. et al. (1995) CD40 cross-linking inhibits specific antibody production by human B cells.
    Int Immunol. 7 (11): 1809-15.
  5. Flavell, D.J. et al. (1995) Preclinical studies with the anti-CD19-saporin immunotoxin BU12-SAPORIN for the treatment of human-B-cell tumours.
    Br J Cancer. 72 (6): 1373-9.
  6. Thornton, C.A. et al. (2002) Expression of CD21 and CD23 during human fetal development.
    Pediatr Res. 52 (2): 245-50.
  7. Vallera, D. A. et al. (2004) Radiotherapy of CD19 expressing Daudi tumors in nude mice with Yttrium-90-labeled anti-CD19 antibody.
    Cancer Biother Radiopharm. 19 (1): 11-23.

UniProt
P15391
Entrez Gene
CD19
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response

MCA2495AMO

149146

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