CD16 antibody | DJ130c

Mouse anti Human CD16:Pacific Blue®

Product Type
Monoclonal Antibody
Clone
DJ130c
Isotype
IgG1
Specificity
CD16

Product Code Applications Pack Size List Price Your Price Qty
MCA2537PB
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B (Scallon et al. 1989). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes (Ravetch and Perussia 1989). In addition, CD16B exists as two allelic variants NA1 and NA2 . DJ130c recognizes all polymorphonuclear cells irrespective of their NA phenotype.

Mouse anti Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012)

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Pacific Blue® 410 455
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Pacific Blue® MCA928PB F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:Pacific Blue®

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD16 antibody

  1. Zhu, X. et al. (1998) Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils.
    J Immunol. 161 (5): 2574-9.
  2. Schmidt, R.E. (1993) CD16 cluster workshop report.
    In Leucocyte Typing V: White cell differentiation antigens, Vol.1. Edited by Schlossman, S.F. et al. Oxford University Press. p805 – 806.
  3. Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
    Diab Vasc Dis Res. 8: 221-8.
  4. Shantsila, E. et al. (2012) Fibrinolytic status in acute coronary syndromes: evidence of differences in relation to clinical features and pathophysiological pathways.
    Thromb Haemost. 108: 32-40.
  5. Shantsila, E. et al. (2011) Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    J Thromb Haemost. 9: 1056-66.
  6. Tapp, L.D. et al. (2012) The CD14++CD16+ monocyte subset and monocyte-platelet interactions in patients with ST-elevation myocardial infarction.
    J Thromb Haemost. 10: 1231-41.
  7. Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
    Arthritis Res Ther. 14: R74.
  8. Ambarus, C.A. et al. (2012) Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.
    J Immunol Methods. 375: 196-206.
  9. View The Latest Product References
  10. Ambarus, C.A. et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.
    PLoS One. 7: e35994.
  11. Shantsila, E. et al. (2012) The effects of exercise and diurnal variation on monocyte subsets and monocyte-platelet aggregates.
    Eur J Clin Invest. 42: 832-9.
  12. Chehadeh. W. et al. (2009) Antibody-mediated opsonization of red blood cells in parvovirus B19 infection.
    Virology. 390: 56-63.
  13. Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
    Circ Heart Fail. 6: 127-35.
  14. Jaipersad, A.S. et al. (2014) Expression of monocyte subsets and angiogenic markers in relation to carotid plaque neovascularization in patients with pre-existing coronary artery disease and carotid stenosis.
    Ann Med. 11: 1-9.
  15. Shantsila, E. et al. (2015) Free Light Chains in patients with acute coronary syndromes: Relationships to inflammation and renal function.
    Int J Cardiol. 185: 322-7.
  16. Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
    Circ Heart Fail. 6 (1): 127-35.
  17. Romee R et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17).
    Blood. 121 (18): 3599-608.
  18. Sousa, S. et al. (2015) Human breast cancer cells educate macrophages toward the M2 activation status.
    Breast Cancer Res. 17: 101.
  19. Shantsila, E. et al. (2019) Mon2 predicts poor outcome in ST-elevation myocardial infarction.
    J Intern Med. 285 (3): 301-16.
  20. Brown, R.A. et al. (2018) Impact of Mon2 monocyte-platelet aggregates on human coronary artery disease.
    Eur J Clin Invest. 48 (5): e12911.

ELISA

Synonyms
FcRIII
UniProt
P08637
O75015
Entrez Gene
FCGR3B
FCGR3A
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0050776 regulation of immune response
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