CD16 antibody | DJ130c











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F ResetMouse anti Human CD16:Pacific Blue®
- Product Type
- Monoclonal Antibody
- Clone
- DJ130c
- Isotype
- IgG1
- Specificity
- CD16
Mouse anti Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B (Scallon et al. 1989). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes (Ravetch and Perussia 1989). In addition, CD16B exists as two allelic variants NA1 and NA2 . DJ130c recognizes all polymorphonuclear cells irrespective of their NA phenotype. Mouse anti Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012) |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Macaque - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Pacific Blue - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Pacific Blue® 410 455 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat | 1/5 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
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- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Pacific Blue® | MCA928PB | F | 100 Tests/1ml |
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List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Pacific Blue® |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for CD16 antibody
-
Schmidt, R.E. (1993) CD16 cluster workshop report.
In Leucocyte Typing V: White cell differentiation antigens, Vol.1. Edited by Schlossman, S.F. et al. Oxford University Press. p805 – 806. -
Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
Diab Vasc Dis Res. 8: 221-8. -
Shantsila, E. et al. (2012) Fibrinolytic status in acute coronary syndromes: evidence of differences in relation to clinical features and pathophysiological pathways.
Thromb Haemost. 108: 32-40. -
Shantsila, E. et al. (2011) Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
J Thromb Haemost. 9: 1056-66. -
Tapp, L.D. et al. (2012) The CD14++CD16+ monocyte subset and monocyte-platelet interactions in patients with ST-elevation myocardial infarction.
J Thromb Haemost. 10: 1231-41. -
Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 14: R74. -
Ambarus, C.A. et al. (2012) Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.
J Immunol Methods. 375: 196-206. -
Ambarus, C.A. et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.
PLoS One. 7: e35994.
View The Latest Product References
-
Shantsila, E. et al. (2012) The effects of exercise and diurnal variation on monocyte subsets and monocyte-platelet aggregates.
Eur J Clin Invest. 42: 832-9. -
Chehadeh. W. et al. (2009) Antibody-mediated opsonization of red blood cells in parvovirus B19 infection.
Virology. 390: 56-63. -
Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
Circ Heart Fail. 6: 127-35. -
Jaipersad, A.S. et al. (2014) Expression of monocyte subsets and angiogenic markers in relation to carotid plaque neovascularization in patients with pre-existing coronary artery disease and carotid stenosis.
Ann Med. 11: 1-9. -
Shantsila, E. et al. (2015) Free Light Chains in patients with acute coronary syndromes: Relationships to inflammation and renal function.
Int J Cardiol. 185: 322-7. -
Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
Circ Heart Fail. 6 (1): 127-35. -
Romee R et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17).
Blood. 121 (18): 3599-608. -
Sousa, S. et al. (2015) Human breast cancer cells educate macrophages toward the M2 activation status.
Breast Cancer Res. 17: 101. -
Shantsila, E. et al. (2019) Mon2 predicts poor outcome in ST-elevation myocardial infarction.
J Intern Med. 285 (3): 301-16. -
Brown, R.A. et al. (2018) Impact of Mon2 monocyte-platelet aggregates on human coronary artery disease.
Eur J Clin Invest. 48 (5): e12911. -
Nakajima-Kato, Y. et al. (2023) A novel monoclonal antibody with improved FcγR blocking ability demonstrated non-inferior efficacy compared to IVIG in cynomolgus monkey ITP model at considerably lower dose.
Clin Exp Immunol. 211 (1): 23-30.
- Synonyms
- FcRIII
- UniProt
- P08637
- O75015
- Entrez Gene
- FCGR3B
- FCGR3A
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0031225 anchored to membrane
- GO:0019864 IgG binding
- GO:0016021 integral to membrane
- GO:0050776 regulation of immune response
MCA2537PB



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