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CD137 antibody | 4B4-1

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Mouse anti Human CD137:APC

Product Type
Monoclonal Antibody
Clone
4B4-1
Isotype
IgG1
Specificity
CD137

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Product Code Applications Pack Size List Price Your Price Qty
MCA6117APC
Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
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Anti Human CD137 Antibody, clone 4B4-1 thumbnail image 1
Anti Human CD137 Antibody, clone 4B4-1 gallery image 1
Surface staining of PHA activated PBMC with anti-CD137:RPE (MCA6117PE)

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Mouse anti Human CD137, clone 4B4-1 recognizes CD137. CD137 (also known as 4-1BB ligand receptor or TNFRSF9) is a type I transmembrane glycoprotein of the Tumor Necrosis Factor receptor family that is expressed on activated T and NK cells, dendritic cells, myeloid cells and at the surface of some non-lymphoid cells under specific conditions (Schwarz et al. 1995). CD137 acts as a receptor for 4-1BB ligand, expressed on activated macrophages, activated B cells, hematopoietic stem cells and myeloid progenitor cells (Pollock et al. 1994) and for some proteins of the extracellular matrix (Chalupny et al. 1992).

The interaction between CD137 and 4-1BBL acts as a co-stimulatory signal for the activation, expansion and survival of T cells (Wen et al. 2002). CD137 signaling is involved in the generation of CD8+ T cells and enhance their cytotoxic activity (Shuford et al. 1997). Because of its ability to promote the anti-tumorigenic activity of cytotoxic T cells, CD137 is the target of several cancer immune therapies, such as the agonist anti-CD137 Urelumab (Segal et al. 2017) and Utomilumab (Chester et al. 2018).
CD137 also binds to the adaptor proteins TRAF1, 2 and 3 to promote NF-kappaB activation (Jang et al. 1998).

Mouse anti-human CD137, clone 4B4-1 has been reported to immunoprecipitate a homodimeric protein of 32kDa under reducing conditions and 85kDa under non-reducing conditions (Garni-Wagner et al. 1996).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Recombinant human CD137 ectodomain
Purity
>95% by SDS PAGE
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
APC 650 661
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the undiluted reagent to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:APC

Further Reading

  1. Chalupny, N.J. et al. (1992) T-cell activation molecule 4-1BB binds to extracellular matrix proteins.
    Proc Natl Acad Sci U S A. 89 (21): 10360-4.
  2. Pollok, K.E. et al. (1994) 4-1BB T-cell antigen binds to mature B cells and macrophages, and costimulates anti-mu-primed splenic B cells.
    Eur J Immunol. 24 (2): 367-74.
  3. Schwarz, H. et al. (1995) ILA, the human 4-1BB homologue, is inducible in lymphoid and other cell lineages.
    Blood. 85 (4): 1043-52.
  4. Garni-wagner, B.A. et al. (1996) 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans.
    Cell Immunol. 169 (1): 91-8.
  5. Shuford, W.W. et al. (1997) 4-1BB costimulatory signals preferentially induce CD8+ T cell proliferation and lead to the amplification in vivo of cytotoxic T cell responses.
    J Exp Med. 186 (1): 47-55.
  6. Wen, T. et al. (2002) 4-1BB ligand-mediated costimulation of human T cells induces CD4 and CD8 T cell expansion, cytokine production, and the development of cytolytic effector function.
    J Immunol. 168 (10): 4897-906.
  7. Segal, N.H. et al. (2017) Results from an Integrated Safety Analysis of Urelumab, an Agonist Anti-CD137 Monoclonal Antibody.
    Clin Cancer Res. 23 (8): 1929-1936.
  8. Chester, C. et al. (2018) Immunotherapy targeting 4-1BB: mechanistic rationale, clinical results, and future strategies.
    Blood. 131 (1): 49-57.

Synonyms
TNFRSF9
UniProt
Q07011
Entrez Gene
TNFRSF9
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0008285 negative regulation of cell proliferation

MCA6117APC

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