CD127 antibody | AbD11590kb

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Human anti Human CD127:RPE

Product Type: Monoclonal Antibody
Clone: AbD11590kb
Isotype: HuCAL/mouse IgG2a Fc
Specificity: CD127

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Product Code Applications Pack Size List Price Your Price Qty

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HCA402PE Datasheet SDS SDS	F	100 Tests		Log in
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Anti Human CD127 Antibody, clone AbD11590kb thumbnail image 1

Anti Human CD127 Antibody, clone AbD11590kb thumbnail image 2

Anti Human CD127 Antibody, clone AbD11590kb gallery image 1 — Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBUV400**) and RPE conjugated Mouse IGg2a isotype control (**MCA929PE**)
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBUV400**) and RPE conjugated Human anti Human CD127 antibody, clone AbD11590kb (**HCA402PE**).
All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the **ZE5 Cell analyser**.

Anti Human CD127 Antibody, clone AbD11590kb gallery image 2 — Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBUV400**) and FITC conjugated Mouse IGg2a isotype control (**MCA929F**).
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBUV400**) and FITC conjugated Human anti Human CD127 antibody, clone AbD11590kb (**HCA402F**).
All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the **ZE5 Cell analyser**.

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Human anti Human CD127 antibody, clone AbD11590kb recognizes the alpha chain of the human interleukin-7 receptor (IL-7R), which is also known as CD127. CD127 is a ~65-75 kDa transmembrane glycoprotein that associates with the interleukin-2 receptor gamma chain to form the functional high affinity IL-7 receptor. CD127 is expressed on lymphoid precursors in the bone marrow, pro-B cells, a subpopulation of thymocytes, T-cells and inflammatory monocytes. CD127 is the specific receptor for IL-7 and the IL-7 receptor plays a critical role in lymphoid development.

Target Species: Human
Product Form: Recombinant chimeric human/mouse IgG2a antibody selected from the HuCAL phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG2a. This antibody is conjugated to R. Phycoerythrin (RPE). Supplied lyophilized.
Reconstitution: Reconstitute with 1 ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source: HKB-11
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Immunogen: Recombinant human IL-7 R alpha/CD127/Fc chimera
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

RPE 488nm laser 496 578

RPE 561nm laser 546 578
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/5

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

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UniProt: P16871
Entrez Gene: IL7R