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CD11b antibody | ICRF44

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MCA551PE
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Mouse anti Human CD11b antibody, clone ICRF44 recognizes the human CD11b cell surface glycoprotein, a 165 kDa molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.

Mouse anti Human CD11b antibody, clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Cynomolgus monkey
Baboon
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Rheumatoid synovial cells and human monocytes.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD11b antibody

  1. Malhotra, V. et al. (1986) Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3).
    Eur J Immunol. 16 (9): 1117-23.
  2. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocyte.
    Leucocyte Typing IV. Oxford University Press 1058-1063.
  3. Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.
    J Cell Biol. 116 (6): 1527-35.
  4. Rezzonico, R. et al. (2000) Engagement of CD11b and CD11c beta2 integrin by antibodies or soluble CD23 induces IL-1beta production on primary human monocytes through mitogen-activated protein kinase-dependent pathways.
    Blood. 95 (12): 3868-77.
  5. Stirling, R.G. et al. (2001) Interleukin-5 induces CD34(+) eosinophil progenitor mobilization and eosinophil CCR3 expression in asthma.
    Am J Respir Crit Care Med. 164: 1403-9.
  6. Canalli, A.A. et al. (2001) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
    Haematologica 96: 526-33.
  7. Rezzonico, R. et al. (2001) Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB.
    Blood. 97 (10): 2932-40.
  8. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
    Clin Exp Immunol. 130: 256-62.
  9. View The Latest Product References
  10. Glasow, A. et al. (2005) Retinoids and myelomonocytic growth factors co-operatively activate RAR{alpha} and induce human myeloid leukemia cell differentiation via MAP kinase pathways.
    Blood 105: 341-9.
  11. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321: 656-62.
  12. Patel, S. et al. (2009) Reconstituted high-density lipoprotein increases plasma high-density lipoprotein anti-inflammatory properties and cholesterol efflux capacity in patients with type 2 diabetes.
    J Am Coll Cardiol. 53: 962-71.
  13. Ramacciotti, E. et al. (2011) Evaluation of soluble p-selectin as a marker for the diagnosis of deep venous thrombosis.
    Clin Appl Thromb Hemost. 17: 425-31.
  14. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One 7: e35577.
  15. Gomes-Alves, P. et al. (2016) In vitro expansion of human cardiac progenitor cells: exploring 'omics tools for characterization of cell-based allogeneic products.
    Transl Res. 171: 96-110.e1-3.
  16. Chen, Y.C. et al. (2018) Effects of normoxic and hypoxic exercise training on the bactericidal capacity and subsequent apoptosis of neutrophils in sedentary men.
    Eur J Appl Physiol. 118 (9): 1985-1995.
  17. Nie, R. et al. (2019) Porphyromonas gingivalis Infection Induces Amyloid-β Accumulation in Monocytes/Macrophages.
    J Alzheimers Dis. 72 (2): 479-94.
  18. Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
    Eur J Med Res. 25 (1): 68.

Flow Cytometry

Functional Assays

Synonyms
Integrin Alpha M Chain
MAC-1
RRID
AB_321291
UniProt
P11215
Entrez Gene
ITGAM
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway

MCA551PE

151677 INN0515

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