CD11a antibody | MEM-25

Mouse anti Human CD11a

Product Type
Monoclonal Antibody
Clone
MEM-25
Isotype
IgG1
Specificity
CD11a

Product Code Applications Pack Size List Price Your Price Qty
MCA6115
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SDS Safety Datasheet SDS
F FN IP 0.1 mg loader
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loader
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Mouse anti Human CD11a antibody, clone MEM-25 recognizes the integrin alphaL subunit, a 170-180 kDa type I transmembrane glycoprotein that associates with CD18 to form the leucocyte function-associated antigen-1, an alphaLbeta2 integrin expressed at the surface of all human leukocyte.

LFA-1 is a receptor for ICAMs, JAM-1 and F11R. Its interaction with endothelial ligands, such as ICAM-1 and JAM-1 is responsible for leukocyte arrest and extravasation (Osterman et al.2002). In addition to its role in leukocyte adhesion and transmigration, LFA-1 is involved in T cell differentiation (Verma et al. 2016, Verman and Kelleher 2017) and is required for the effective lysis of infected cells by cytotoxic T cells (Anikeeva et al. 2005, Pontes Ferreira et al. 2017). LFA-1 also contributes to NK cell cytotoxicity (Domingo et al. 2004) and participates to the clearance of apoptotic neutrophils by macrophages (Kristóf et al. 2013). The interaction of LFA-1 with ICAM-1 is the target of Lifitegrast, a drug approved for the treatment of Dry Eye Disease (Abidi et al. 2016).

Mouse anti Human CD11 antibody, clone MEM-25 inhibits the cytotoxic activity of NK cells (Bazil et al. 1990).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Leucocytes from a patient suffering from a LGL-type leukemia
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/50
Functional Assays
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD11a antibody

  1. Ostermann, G. et al. (2002) JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes.
    Nat Immunol. 3 (2): 151-8.
  2. Verma, N.K. et al. (2016) LFA-1/ICAM-1 Ligation in Human T Cells Promotes Th1 Polarization through a GSK3β Signaling-Dependent Notch Pathway.
    J Immunol. 197 (1): 108-18.
  3. Verma, N.K. & Kelleher, D. (2017) Not Just an Adhesion Molecule: LFA-1 Contact Tunes the T Lymphocyte Program.
    J Immunol. 199 (4): 1213-1221.
  4. Anikeeva, N. et al. (2005) Distinct role of lymphocyte function-associated antigen-1 in mediating effective cytolytic activity by cytotoxic T lymphocytes.
    Proc Natl Acad Sci U S A. 102 (18): 6437-42.
  5. Ferreira, C.P. et al. (2017) LFA-1 Mediates Cytotoxicity and Tissue Migration of Specific CD8+ T Cells after Heterologous Prime-Boost Vaccination against Trypanosoma cruzi Infection.
    Front Immunol. 8: 1291.
  6. Barber, D.F. et al. (2004) LFA-1 contributes an early signal for NK cell cytotoxicity.
    J Immunol. 173 (6): 3653-9.
  7. Abidi, A. et al. (2016) Lifitegrast: A novel drug for treatment of dry eye disease.
    J Pharmacol Pharmacother. 7 (4): 194-198.
  8. Bazil, V. et al. (1990) Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.
    Folia Biol (Praha). 36 (1): 41-50.
  9. View The Latest Product References

Synonyms
Integrin Alpha L Chain
LFA-1
UniProt
P20701
Entrez Gene
ITGAL
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
GO:0007596 blood coagulation
GO:0050839 cell adhesion molecule binding
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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