CD11a antibody | MEM-25
Mouse anti Human CD11a:APC
Mouse anti Human CD11a:FITC
Mouse anti Human CD11a:Low Endotoxin
Mouse anti Human CD11a
Mouse anti Human CD11a:RPE
- Product Type
- Monoclonal Antibody
- Clone
- MEM-25
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6115APC | F | 100 Tests/1ml |
|
|
| MCA6115F | F | 100 Tests/2ml |
|
|
| MCA6115EL | F FN IP | 0.1 mg |
|
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| MCA6115 | F FN IP | 0.1 mg |
|
|
| MCA6115PE | F | 100 Tests/2ml |
|
Mouse anti Human CD11a antibody, clone MEM-25 recognizes the integrin alphaL subunit, a 170-180 kDa type I transmembrane glycoprotein that associates with CD18 to form the leucocyte function-associated antigen-1, an alphaLbeta2 integrin expressed at the surface of all human leukocyte.
LFA-1 is a receptor for ICAMs, JAM-1 and F11R. Its interaction with endothelial ligands, such as ICAM-1 and JAM-1 is responsible for leukocyte arrest and extravasation (Osterman et al.2002). In addition to its role in leukocyte adhesion and transmigration, LFA-1 is involved in T cell differentiation (Verma et al. 2016, Verman and Kelleher 2017) and is required for the effective lysis of infected cells by cytotoxic T cells (Anikeeva et al. 2005, Pontes Ferreira et al. 2017). LFA-1 also contributes to NK cell cytotoxicity (Domingo et al. 2004) and participates to the clearance of apoptotic neutrophils by macrophages (Kristóf et al. 2013). The interaction of LFA-1 with ICAM-1 is the target of Lifitegrast, a drug approved for the treatment of Dry Eye Disease (Abidi et al. 2016).
Mouse anti Human CD11 antibody, clone MEM-25 inhibits the cytotoxic activity of NK cells (Bazil et al. 1990).
LFA-1 is a receptor for ICAMs, JAM-1 and F11R. Its interaction with endothelial ligands, such as ICAM-1 and JAM-1 is responsible for leukocyte arrest and extravasation (Osterman et al.2002). In addition to its role in leukocyte adhesion and transmigration, LFA-1 is involved in T cell differentiation (Verma et al. 2016, Verman and Kelleher 2017) and is required for the effective lysis of infected cells by cytotoxic T cells (Anikeeva et al. 2005, Pontes Ferreira et al. 2017). LFA-1 also contributes to NK cell cytotoxicity (Domingo et al. 2004) and participates to the clearance of apoptotic neutrophils by macrophages (Kristóf et al. 2013). The interaction of LFA-1 with ICAM-1 is the target of Lifitegrast, a drug approved for the treatment of Dry Eye Disease (Abidi et al. 2016).
Mouse anti Human CD11 antibody, clone MEM-25 inhibits the cytotoxic activity of NK cells (Bazil et al. 1990).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant and 0.2 μm filter sterilized
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Leucocytes from a patient suffering from a LGL-type leukemia
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20701
- Entrez Gene
- ITGAL
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
- GO:0007596 blood coagulation
- GO:0050839 cell adhesion molecule binding
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
- Regulatory
- For research purposes only
Applications of CD11a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/50 | |
| Functional Assays | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/50 | |
| Functional Assays | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the undiluted product to label 1x106 cells in 100ul
- Flow Cytometry
- Use 20ul of the undiluted reagent to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 20ul of the undiluted reagent to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
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| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
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