CD11a antibody | MEM-25
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F ResetMouse anti Human CD11a
- Product Type
- Monoclonal Antibody
- Clone
- MEM-25
- Isotype
- IgG1
- Specificity
- CD11a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD11a antibody, clone MEM-25 recognizes the integrin alphaL subunit, a 170-180 kDa type I transmembrane glycoprotein that associates with CD18 to form the leucocyte function-associated antigen-1, an alphaLbeta2 integrin expressed at the surface of all human leukocyte. LFA-1 is a receptor for ICAMs, JAM-1 and F11R. Its interaction with endothelial ligands, such as ICAM-1 and JAM-1 is responsible for leukocyte arrest and extravasation (Osterman et al.2002). In addition to its role in leukocyte adhesion and transmigration, LFA-1 is involved in T cell differentiation (Verma et al. 2016, Verman and Kelleher 2017) and is required for the effective lysis of infected cells by cytotoxic T cells (Anikeeva et al. 2005, Pontes Ferreira et al. 2017). LFA-1 also contributes to NK cell cytotoxicity (Domingo et al. 2004) and participates to the clearance of apoptotic neutrophils by macrophages (Kristóf et al. 2013). The interaction of LFA-1 with ICAM-1 is the target of Lifitegrast, a drug approved for the treatment of Dry Eye Disease (Abidi et al. 2016). Mouse anti Human CD11 antibody, clone MEM-25 inhibits the cytotoxic activity of NK cells (Bazil et al. 1990). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Leucocytes from a patient suffering from a LGL-type leukemia
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/50 |
| Functional Assays | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD11a antibody
-
Ostermann, G. et al. (2002) JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes.
Nat Immunol. 3 (2): 151-8. -
Verma, N.K. et al. (2016) LFA-1/ICAM-1 Ligation in Human T Cells Promotes Th1 Polarization through a GSK3β Signaling-Dependent Notch Pathway.
J Immunol. 197 (1): 108-18. -
Verma, N.K. & Kelleher, D. (2017) Not Just an Adhesion Molecule: LFA-1 Contact Tunes the T Lymphocyte Program.
J Immunol. 199 (4): 1213-1221. -
Anikeeva, N. et al. (2005) Distinct role of lymphocyte function-associated antigen-1 in mediating effective cytolytic activity by cytotoxic T lymphocytes.
Proc Natl Acad Sci U S A. 102 (18): 6437-42. -
Ferreira, C.P. et al. (2017) LFA-1 Mediates Cytotoxicity and Tissue Migration of Specific CD8+ T Cells after Heterologous Prime-Boost Vaccination against Trypanosoma cruzi Infection.
Front Immunol. 8: 1291. -
Barber, D.F. et al. (2004) LFA-1 contributes an early signal for NK cell cytotoxicity.
J Immunol. 173 (6): 3653-9. -
Abidi, A. et al. (2016) Lifitegrast: A novel drug for treatment of dry eye disease.
J Pharmacol Pharmacother. 7 (4): 194-198. -
Bazil, V. et al. (1990) Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.
Folia Biol (Praha). 36 (1): 41-50.
View The Latest Product References
- Synonyms
- Integrin Alpha L Chain
- LFA-1
- UniProt
- P20701
- Entrez Gene
- ITGAL
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
- GO:0007596 blood coagulation
- GO:0050839 cell adhesion molecule binding
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- View More GO Terms
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
MCA6115
149827
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