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CD106 antibody | 1.G11B1

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Anti Human CD106 Antibody, clone 1.G11B1 thumbnail image 1 Anti Human CD106 Antibody, clone 1.G11B1 thumbnail image 2 Anti Human CD106 Antibody, clone 1.G11B1 thumbnail image 3 Anti Human CD106 Antibody, clone 1.G11B1 thumbnail image 4 Anti Human CD106 Antibody, clone 1.G11B1 thumbnail image 5 Anti Human CD106 Antibody, clone 1.G11B1 thumbnail image 6
Anti Human CD106 Antibody, clone 1.G11B1 gallery image 1
Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and Alexa Fluor 647 conjugated Mouse anti Human CD106 (MCA907A647). Figure B. Cells stimulated with TNFa for 19 hours were stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and Alexa Fluor 647 conjugated Mouse IgG (MCA928A647). Figure C. Cells stimulated with TNFa for 19 hours were stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and Alexa Fluor 647 conjugated Mouse anti Human CD106 (MCA907A647). All experiments performed on HUVECs gated on single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD106 Antibody, clone 1.G11B1 gallery image 2
Figure A. Unstimulated cells were stained with PE conjugated Mouse anti Human CD31 (MCA1738PE) and Alexa Fluor 700 conjugated Mouse anti Human CD106 (MCA907A700). Figure B. Cells stimulated with TNFa for 19 hours were stained with PE conjugated Mouse anti Human CD31 (MCA1738PE) and Alexa Fluor 700 conjugated Mouse IgG (MCA928A700). Figure C. Cells stimulated with TNFa for 19 hours were stained with PE conjugated Mouse anti Human CD31 (MCA1738PE) and Alexa Fluor 700 conjugated Mouse anti Human CD106 (MCA907A700). All experiments performed on HUVECs gated on single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD106 Antibody, clone 1.G11B1 gallery image 3
Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and Pacific Blue conjugated Mouse anti Human CD106 (MCA907PB). Figure B. Cells stimulated with TNFa for 19 hours were stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and Pacific Blue conjugated Mouse IgG (MCA928PB). Figure C. Cells stimulated with TNFa for 19 hours were stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and Pacific Blue conjugated Mouse anti Human CD106 (MCA907PB). All experiments performed on HUVECs gated on single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD106 Antibody, clone 1.G11B1 gallery image 4
Figure A. Unstimulated cells stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and RPE conjugated Mouse anti Human CD106 (MCA907PE). Figure B. Cells stimulated with TNFα for 19 h then stained with FITC conjugated Mouse anti Human CD31 (MCA1738F) and RPE conjugated Mouse anti Human CD106 (MCA907PE). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD106 Antibody, clone 1.G11B1 gallery image 5
Published customer image:
FITC conjugated Mouse anti Human CD106 antibody, clone 1.G11B1 (MCA907F) used for the evaluation of VCAM-1 expression on mesenchymal stem cells by flow cytometry.
Image caption:
Immunophenotyping profile of MSC under 21% O2 (A) and 1% O2 (B) by flow cytometric analysis of cell surface marker expression after 14 days showed no changes in immunophenotype of MSC due to varying oxygen concentrations (n = 3).

From: Holzwarth C, Vaegler M, Gieseke F, Pfister SM, Handgretinger R, Kerst G, Müller I.
Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.
BMC Cell Biol. 2010 Jan 28;11:11.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD106 Antibody, clone 1.G11B1 gallery image 6
Published customer image:
Mouse anti Human CD106 antibody, clone 1.G11B1 (MCA907F) used to evaluate VCAM-1 expression on cellular microparticles by flow cytometry.
Image caption:
a The CD105:CD106 MP ratio across individual studies. Groups were PCOS 1 groups 1–4, T2D groups 1 and 2 and control groups 1 and 2. Error bars represent SEM. b Box plot showing combined data for PCOS, T2D and controls groups. Horizontal bar within the box represents the median, ‘x’ denotes mean

From: Al-Qaissi A, Alqarni S, Javed Z, Atkin SL, Sathyapalan T, Vince RV, Madden LA.
The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects.
Endocrine. 2019 Nov;66(2):220-225.
doi: 10.1007/s12020-019-02059-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human CD106:RPE

Product Type
Monoclonal Antibody
Clone
1.G11B1
Isotype
IgG1
Specificity
CD106

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Product Code Applications Pack Size List Price Your Price Qty
MCA907PE
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F 100 Tests loader
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Mouse anti Human CD106 antibody, clone 1.G11B1 recognizes human VCAM-1, a ~110 kDa molecule whose ligand is VLA4. The antigen is expressed on activated endothelial cells and on some tissue macrophages, bone marrow fibroblasts and myoblasts.

Mouse anti Human CD106 antibody, clone 1.G11B1 inhibits cellular adhesion mediated by VCAM-1 (Patel 1998).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select combined or multi-laser view to visualize the spectra

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Description Mouse IgG1 Negative Control:RPE

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Human Seroblock BUF070B F 200 Test
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References for CD106 antibody

  1. Thornhill, M.H. et al. (1991) Tumor necrosis factor combines with IL-4 or IFN-gamma to selectively enhance endothelial cell adhesiveness for T cells. The contribution of vascular cell adhesion molecule-1-dependent and -independent binding mechanisms.
    J Immunol. 146 (2): 592-8.
  2. Rosenman, S.J. et al. (1995) Cytokine-induced expression of vascular cell adhesion molecule-1 (VCAM-1) by astrocytes and astrocytoma cell lines.
    J Immunol. 154 (4): 1888-99.
  3. Reparon-Schuijt, C.C. et al. (2000) Regulation of synovial B cell survival in rheumatoid arthritis by vascular cell adhesion molecule 1 (CD106) expressed on fibroblast-like synoviocytes.
    Arthritis Rheum. 43 (5): 1115-21.
  4. Ali, S. et al. (2000) Intercellular cell adhesion molecule-1, vascular cell adhesion molecule-1, and regulated on activation normal T cell expressed and secreted are expressed by human breast carcinoma cells and support eosinophil adhesion and activation.
    Am J Pathol. 157: 313-21.
  5. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.
    J Pharmacol Exp Ther. 302 (3): 861-70.
  6. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
    Kidney Int. 62: 654-67.
  7. Cabeza, N. et al. (2004) Surface expression of collagen receptor Fc receptor-gamma/glycoprotein VI is enhanced on platelets in type 2 diabetes and mediates release of CD40 ligand and activation of endothelial cells.
    Diabetes 53: 2117-21.
  8. Dunoyer-Geindre, S. et al. (2004) Aspirin inhibits endothelial cell activation induced by antiphospholipid antibodies.
    J Thromb Haemost. 2: 1176-81.
    9. View The Latest Product References
  9. Pelletier M, Girard D. (2005) Interleukin-15 increases neutrophil adhesion onto human respiratory epithelial A549 cells and attracts neutrophils in vivo.
    Clin Exp Immunol. 141: 315-25.
  10. Peterson, M.D. et al. (2005) Monocyte-induced endothelial calcium signaling mediates early xenogeneic endothelial activation.
    Am J Transplant 5 (2): 237-47.
  11. Kindle, L. et al. (2005) Human microvascular endothelial cell activation by IL-1 and TNF-alpha stimulates the adhesion and transendothelial migration of circulating human CD14+ monocytes that develop with RANKL into functional osteoclasts.
    J Bone Miner Res. 21: 193-206.
  12. Corvaisier, M. et al. (2005) V gamma 9V delta 2 T cell response to colon carcinoma cells.
    J Immunol. 175: 5481-8.
  13. Kahler, C.M. (2007) Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury.
    Respir Res. 8: 50.
  14. Holzwarth, C. et al. (2010) Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells.
    BMC Cell Biol. 11: 11.
  15. Ruschulte, H. et al. (2011) Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro.
    BMC Res Notes. 4: 40.
  16. May, R.D. et al. (2012) Preclinical development of CAT-354, an IL-13 neutralizing antibody, for the treatment of severe uncontrolled asthma.
    Br J Pharmacol. 166 (1): 177-93.
  17. Murphy, A.J. et al. (2013) Anti-inflammatory functions of apolipoprotein a-I and high-density lipoprotein are preserved in trimeric apolipoprotein a-I.
    J Pharmacol Exp Ther. 344: 41-9.
  18. Chadderdon, S.M. et al. (2014) Proinflammatory endothelial activation detected by molecular imaging in obese nonhuman primates coincides with onset of insulin resistance and progressively increases with duration of insulin resistance.
    Circulation. 129 (4): 471-8.
  19. Old, E.A. et al. (2014) Monocytes expressing CX3CR1 orchestrate the development of vincristine-induced pain.
    J Clin Invest. 124 (5): 2023-36.
  20. Zhang, J. et al. (2016) Bone mesenchymal stem cells differentiate into myofibroblasts in the tumor microenvironment.
    Oncol Lett. 12 (1): 644-50.
  21. Lim, J.L. et al. (2016) Protective effects of monomethyl fumarate at the inflamed blood-brain barrier.
    Microvasc Res. 105: 61-9.
  22. Schmidt, M. et al. (2016) Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis.
    Methods Mol Biol. 1390: 319-40.
  23. Al-Qaissi, A. et al. (2019) The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects.
    Endocrine. 66 (2): 220-225.
  24. Lauranzano, E. et al. (2019) A Microfluidic Human Model of Blood-Brain Barrier Employing Primary Human Astrocytes.
    Adv Biosyst. 3 (7): e1800335.
  25. Hara, H. et al. (2021) Stable expression of the human thrombomodulin transgene in pig endothelial cells is associated with a reduction in the inflammatory response.
    Cytokine. 148: 155580.
  26. Kohs, T.C.L. et al. (2022) Ibrutinib Inhibits BMX-Dependent Endothelial VCAM-1 Expression In Vitro and Pro-Atherosclerotic Endothelial Activation and Platelet Adhesion In Vivo.
    Cell Mol Bioeng. 15 (3): 231-43.
  27. Connolly, D.M. et al. (2023) Early Human Pathophysiological Responses to Exertional Hypobaric Decompression Stress.
    Aerosp Med Hum Perform. 94 (10): 738-49.
  28. Bacci, M. et al. (2023) Development of Personalized Thrombogenesis and Thrombin Generation Assays to Assess Endothelial Dysfunction in Cardiovascular Diseases.
    Biomedicines. 11 (6):1669.

Further Reading

  1. Kong, D.H. et al. (2018) Emerging Roles of Vascular Cell Adhesion Molecule-1 (VCAM-1) in Immunological Disorders and Cancer.
    Int J Mol Sci. 19 (4): 1057.

ELISA

Flow Cytometry

Functional Assays

Western Blotting

Synonyms
VCAM-1
UniProt
P19320
Entrez Gene
VCAM1
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0005902 microvillus
GO:0009897 external side of plasma membrane
GO:0022614 membrane to membrane docking
GO:0030175 filopodium
GO:0030183 B cell differentiation
GO:0042102 positive regulation of T cell proliferation
GO:0044419 interspecies interaction between organisms
GO:0045177 apical part of cell
GO:0050776 regulation of immune response
GO:0050901 leukocyte tethering or rolling
GO:0060333 interferon-gamma-mediated signaling pathway

MCA907PE

INN1611

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