CCT Beta antibody | PK/8/4/4i/2F

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Rat anti CCT Beta

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Rat anti Human CCT beta antibody recognizes human T-complex protein 1 subunit beta, also known as CCT beta, CCT2 or TCP-1-beta. CCT beta is a 535 amino acid cytoplasmically expressed chaperonin molecule involved in protein folding and has been shown to be involved in actin interaction (Llorca et al. 1999). Two isoforms of CCT beta have been described, arising from alternative splicing. It is not clear whether this Rat anti Human CCT beta antibody recognizes the shorter isoform 2, lacking the N-terminal 47 amino acids seen in isoform 1.

Rat anti Human CCT beta antibody recognizes CCT beta as a single band of ~ 57 kDa in multiple human cell line lysates by western blotting under reducing conditions and is cross reactive with both mouse and rat cell line lysates.

Product Details

Target Species
Western Blotting
Anti CCT beta antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Mouse CCT beta peptide conjugated to purified tuberculin protein derivative
0.5 mg/ml

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0051082 unfolded protein binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0051084 'de novo' posttranslational protein folding
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of CCT Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader

Application Based External Images


Western Blotting

Product Specific References

References for CCT Beta antibody

  1. Satish, L. et al. (2010) Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds.
    Cell Stress Chaperones. 15 (6): 819-26.
  2. Fislová, T. et al. (2010) Association of the influenza virus RNA polymerase subunit PB2 with the host chaperonin CCT.
    J Virol. 84 (17): 8691-9.
  3. Satish, L. et al. (2010) Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is a specific regulator of fibroblast motility and contractility.
    PLoS One. 5 (4): e10063.
  4. Plimpton, R.L. et al. (2015) Structures of the Gβ-CCT and PhLP1-Gβ-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγ dimer assembly.
    Proc Natl Acad Sci U S A. 112 (8): 2413-8.
  5. Satish, L. et al. (2011) Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
    Cell Stress Chaperones. 16 (6): 675-80.

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