CCT Beta antibody | PK/8/4/4i/2F

Rat anti CCT Beta

Product Type
PrecisionAb Monoclonal
Clone
PK/8/4/4i/2F
Isotype
IgG2b
Format
Purified
Specificity
CCT Beta

Product Code Applications Pack Size List Price Your Price Qty
VMA00026
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WB 100 µl loader
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Rat anti Human CCT beta antibody recognizes human T-complex protein 1 subunit beta, also known as CCT beta, CCT2 or TCP-1-beta. CCT beta is a 535 amino acid cytoplasmically expressed chaperonin molecule involved in protein folding and has been shown to be involved in actin interaction (Llorca et al. 1999). Two isoforms of CCT beta have been described, arising from alternative splicing. It is not clear whether this Rat anti Human CCT beta antibody recognizes the shorter isoform 2, lacking the N-terminal 47 amino acids seen in isoform 1.

Rat anti Human CCT beta antibody recognizes CCT beta as a single band of ~ 57 kDa in multiple human cell line lysates by western blotting under reducing conditions and is cross reactive with both mouse and rat cell line lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti CCT beta antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
Bovine
Chicken
Sheep
Dog
Rabbit
Hamster
Pig
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Mouse CCT beta peptide conjugated to purified tuberculin protein derivative
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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References for CCT Beta antibody

  1. Satish, L. et al. (2010) Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds.
    Cell Stress Chaperones. 15 (6): 819-26.
  2. Fislová, T. et al. (2010) Association of the influenza virus RNA polymerase subunit PB2 with the host chaperonin CCT.
    J Virol. 84 (17): 8691-9.
  3. Satish, L. et al. (2010) Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is a specific regulator of fibroblast motility and contractility.
    PLoS One. 5 (4): e10063.
  4. Plimpton, R.L. et al. (2015) Structures of the Gβ-CCT and PhLP1-Gβ-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγ dimer assembly.
    Proc Natl Acad Sci U S A. 112 (8): 2413-8.
  5. Satish, L. et al. (2011) Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
    Cell Stress Chaperones. 16 (6): 675-80.

Immunoprecipitation

Western Blotting

UniProt
P78371
Entrez Gene
CCT2
GO Terms
GO:0051082 unfolded protein binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0051084 'de novo' posttranslational protein folding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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