Mouse anti Human CCL3 antibody, clone H03-5C10, recognizes C-C motif chemokine 3 (CCL3) also known as G0/G1 switch regulatory protein 19-1 and Macrophage inflammatory protein 1 alpha (MIP1α). CCL3 is an inflammatory cytokine with the ability to activate chemotactic mobilization of monocyte and lymphocyte cells. It also plays a role in regulating the proliferation of hematopoietic stem/progenitor cells (Baba & Mukaida 2014). The receptors of CCL3 are the G-protein coupled receptors CCR1, CCR5 and the D6 receptor in humans. Signaling via CCR1 and CCR5, CCL3 is able to stimulate lymphocyte recruitment to sites of infection, as well as CD8+ T cells to active lymph nodes (Staversky et al. 2018).

Expression levels of CCL3 have been reported to increase in patients with colorectal cancer, independently of tumor stage. This has been shown to be concurrent with the upregulated expression of CCR1 and CCR5 (Lόpez et al. 2018). CCL3 has also been implicated in various pathologies related to microenvironmental dysfunction, including myelodysplastic syndrome, metastatic disease of renal cell carcinoma and B cell lymphomas (Staversky et al. 2018).

The biotinylated Mouse anti Human CCL3 antibody, clone F01.1-3E5 (MCA6221B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CCL3 antibody, clone H03-5C10 (MCA6220GA) as the capture antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1.0 ug/ml