CCL3 antibody | F01.1-3E5
Mouse anti Human CCL3 antibody, clone H03-5C10, recognizes C-C motif chemokine 3 (CCL3) also known as G0/G1 switch regulatory protein 19-1 and Macrophage inflammatory protein 1 alpha (MIP1α). CCL3 is an inflammatory cytokine with the ability to activate chemotactic mobilization of monocyte and lymphocyte cells. It also plays a role in regulating the proliferation of hematopoietic stem/progenitor cells (Baba & Mukaida 2014). The receptors of CCL3 are the G-protein coupled receptors CCR1, CCR5 and the D6 receptor in humans. Signaling via CCR1 and CCR5, CCL3 is able to stimulate lymphocyte recruitment to sites of infection, as well as CD8+ T cells to active lymph nodes (Staversky et al. 2018).
Expression levels of CCL3 have been reported to increase in patients with colorectal cancer, independently of tumor stage. This has been shown to be concurrent with the upregulated expression of CCR1 and CCR5 (Lόpez et al. 2018). CCL3 has also been implicated in various pathologies related to microenvironmental dysfunction, including myelodysplastic syndrome, metastatic disease of renal cell carcinoma and B cell lymphomas (Staversky et al. 2018).
The biotinylated Mouse anti Human CCL3 antibody, clone F01.1-3E5 (MCA6221B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CCL3 antibody, clone H03-5C10 (MCA6220GA) as the capture antibody.
Expression levels of CCL3 have been reported to increase in patients with colorectal cancer, independently of tumor stage. This has been shown to be concurrent with the upregulated expression of CCR1 and CCR5 (Lόpez et al. 2018). CCL3 has also been implicated in various pathologies related to microenvironmental dysfunction, including myelodysplastic syndrome, metastatic disease of renal cell carcinoma and B cell lymphomas (Staversky et al. 2018).
The biotinylated Mouse anti Human CCL3 antibody, clone F01.1-3E5 (MCA6221B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CCL3 antibody, clone H03-5C10 (MCA6220GA) as the capture antibody.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- E. coli derived recombinant human full length CCL3 (Ala27-Ala92)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P10147
- Entrez Gene
- CCL3
- GO Terms
- GO:0007010 cytoskeleton organization
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006874 cellular calcium ion homeostasis
- GO:0006887 exocytosis
- GO:0006928 cellular component movement
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0007267 cell-cell signaling
- GO:0008009 chemokine activity
- GO:0042056 chemoattractant activity
- GO:0045069 regulation of viral genome replication
- Regulatory
- For research purposes only
Applications of CCL3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Human CCL3 | MCA6220GA | E | 0.1 mg |
|
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