CAND1 antibody | CD01/4H2

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siRNA Validated

IP-MS Validated

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CAND1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 16 second exposure. Arrow points to CAND1 (molecular weight 136 kDa)

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CAND1 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti CAND1 antibody (**VMA00572**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 10 second (chemiluminescent channel) and 10 second (rhodamine channel) exposure.

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of CAND1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against CAND1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Anti CAND1 Antibody, clone CD01/4H2 (PrecisionAb Monoclonal Antibody) gallery image 4 — Immunoprecipitation of CAND1 from HEK293 whole cell lysate. The Input lane is 10% input; the CAND1-IP lane is the immunoprecipitation with the CAND1 antibody (2.5 µg, **VMA00572**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA691). Western blot analysis was performed using CAND1 antibody (1/1,000, **VMA00572**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CAND1.

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WB IP

Mouse anti CAND1

Product Type: PrecisionAb Monoclonal
Clone: CD01/4H2
Isotype: IgG2b
Format: Purified
Specificity: CAND1

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VMA00572 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human CAND1 antibody recognizes the cullin-associated NEDD8-dissociated protein 1, also known as TBP interacting protein, TBP-interacting protein 120A, TBP-interacting protein of 120 kDa A, cullin-associated NEDD8-dissociated protein 1, cullin-associated and neddylation-dissociated protein 1 or p120 CAND1.

Mouse anti Human CAND1 antibody detects a band of 136 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CAND1 detects a band of approximately 136 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human CAND1 (amino acids 1 - 100)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.