BIRC2 antibody | AB01/3B4

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Anti BIRC2 Antibody, clone AB01/3B4 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with BIRC2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to BIRC2 (molecular weight 70 kDa)

Mouse anti BIRC2

Product Type: PrecisionAb™ Monoclonal
Clone: AB01/3B4
Isotype: IgG2b
Format: Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VMA00532	WB			100 µl

Summary
Secondary Antibodies

Mouse anti Human BIRC2 antibody recognizes the baculoviral IAP repeat-containing protein 2, also known as IAP homolog B, cIAP1, RING finger protein 48, TNFR2-TRAF-signaling complex protein 2, apoptosis inhibitor 1 or inhibitor of apoptosis protein 2.

The protein encoded by BIRC2 gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for BIRC2 (provided by RefSeq, Jan 2012).

Mouse anti Human BIRC2 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti BIRC2 detects a band of approximately 70 kDa in K562 cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: E. coli-derived recombinant human BIRC2 (amino acids 1 - 618)
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q13490 Related reagents
Entrez Gene: BIRC2 Related reagents
GO Terms: GO:0000209 protein polyubiquitination; GO:0005829 cytosol; GO:0004842 ubiquitin-protein ligase activity; GO:0006921 cellular component disassembly involved in apoptosis; GO:0007166 cell surface receptor linked signaling pathway; GO:0008270 zinc ion binding; GO:0009898 internal side of plasma membrane; GO:0035631 CD40 receptor complex; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043161 proteasomal ubiquitin-dependent protein catabolic process; GO:0047485 protein N-terminus binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of BIRC2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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