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BIRC2 antibody | AB01/3B4

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siRNA Validated
Anti BIRC2 Antibody, clone AB01/3B4 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti BIRC2 Antibody, clone AB01/3B4 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti BIRC2 Antibody, clone AB01/3B4 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti BIRC2 Antibody, clone AB01/3B4 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with BIRC2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to BIRC2 (molecular weight 70 kDa)
Anti BIRC2 Antibody, clone AB01/3B4 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against BIRC2 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti BIRC2 antibody (VMA00532) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 60 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.
Anti BIRC2 Antibody, clone AB01/3B4 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of BIRC2 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against BIRC2 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti BIRC2

Product Type
PrecisionAb Monoclonal
Clone
AB01/3B4
Isotype
IgG2b
Format
Purified
Specificity
BIRC2

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Technical Suppport
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VMA00532
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WB 100 µl loader
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Mouse anti Human BIRC2 antibody recognizes the baculoviral IAP repeat-containing protein 2, also known as IAP homolog B, cIAP1, RING finger protein 48, TNFR2-TRAF-signaling complex protein 2, apoptosis inhibitor 1 or inhibitor of apoptosis protein 2.

The protein encoded by BIRC2 gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for BIRC2 (provided by RefSeq, Jan 2012).

Mouse anti Human BIRC2 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti BIRC2 detects a band of approximately 70 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human BIRC2 (amino acids 1 - 618)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13490
Entrez Gene
BIRC2
GO Terms
GO:0000209 protein polyubiquitination
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0008270 zinc ion binding
GO:0009898 internal side of plasma membrane
GO:0035631 CD40 receptor complex
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0047485 protein N-terminus binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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