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BAK antibody | AB04/1A2

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Anti BAK Antibody, clone AB04/1A2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti BAK Antibody, clone AB04/1A2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti BAK Antibody, clone AB04/1A2 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti BAK Antibody, clone AB04/1A2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with BAK antibody (VMA00527) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to BAK (molecular weight 25 kDa).
Anti BAK Antibody, clone AB04/1A2 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of BAK knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti BAK antibody (VMA00527) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 24 second and (B) 30 second exposure.
Anti BAK Antibody, clone AB04/1A2 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of BAK from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti BAK

Product Type
PrecisionAb Monoclonal
Clone
AB04/1A2
Isotype
IgG2a
Format
Purified
Specificity
BAK

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Product Code Applications Pack Size List Price Your Price Qty
VMA00527
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SDS Safety Datasheet SDS
IF WB 100 µl loader
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loader

Mouse anti Human BAK antibody recognizes the Bcl-2 homologous antagonist/killer, also known as BAK, BCL2-like 7 protein, apoptosis regulator BAK, bcl2-L-7 or pro-apoptotic protein BAK.

The protein encoded by BAK1 gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress (provided by RefSeq, Jul 2008).

Mouse anti Human BAK antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti BAK detects a band of approximately 25 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human BAK (amino acids 29 - 187)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q16611
Entrez Gene
BAK1
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0046872 metal ion binding
GO:0005741 mitochondrial outer membrane
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042802 identical protein binding
GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
GO:0046930 pore complex
GO:0043496 regulation of protein homodimerization activity
GO:0043497 regulation of protein heterodimerization activity
GO:0046902 regulation of mitochondrial membrane permeability
GO:0046982 protein heterodimerization activity
GO:0051881 regulation of mitochondrial membrane potential

VMA00527

170420

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