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ATF2 antibody

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Western Blot Validated
Anti ATF2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ATF2 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to ATF2 (molecular weight 65-75 kDa).

Mouse anti ATF2

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00671
Datasheet
WB
SDS
100 µl loader

Mouse anti Human ATF2 antibody recognizes the cyclic AMP-dependent transcription factor ATF2, also known as activating transcription factor 2 splice variant ATF2-var2, cAMP response element-binding protein CRE-BP1, cAMP responsive element binding protein 2, cAMP-dependent transcription factor ATF-2 or histone acetyltransferase ATF2.

ATF2 encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Several alternatively spliced transcript variants have been found for ATF2 (provided by RefSeq, Jan 2012).

Mouse anti Human ATF2 antibody detects bands of 65-75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti ATF2 antibody detects bands of approximately 86-75 kDa in A549 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Partial recombinant of human ATF2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P15336
Entrez Gene
ATF2
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0008270 zinc ion binding
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043565 sequence-specific DNA binding
GO:0045087 innate immune response
GO:0046983 protein dimerization activity
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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