ATF2 antibody
Mouse anti ATF2
ATF2 antibody recognizes the cyclic AMP-dependent transcription factor ATF-2, also known as activating transcription factor 2 splice variant ATF2-var2, cAMP response element-binding protein CRE-BP1, cAMP responsive element binding protein 2 or histone acetyltransferase ATF2.
ATF2 Antibody with Control Lysate
ATF2 antibody recognizes the cyclic AMP-dependent transcription factor ATF-2, also known as activating transcription factor 2 splice variant ATF2-var2, cAMP response element-binding protein CRE-BP1, cAMP responsive element binding protein 2 or histone acetyltransferase ATF2.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00671 | WB | 100 µl | ||||
| VMA00671KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ATF2 antibody recognizes the cyclic AMP-dependent transcription factor ATF2, also known as activating transcription factor 2 splice variant ATF2-var2, cAMP response element-binding protein CRE-BP1, cAMP responsive element binding protein 2, cAMP-dependent transcription factor ATF-2 or histone acetyltransferase ATF2.
ATF2 encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Several alternatively spliced transcript variants have been found for ATF2 (provided by RefSeq, Jan 2012).
Mouse anti Human ATF2 antibody detects bands of 65-75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
ATF2 encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Several alternatively spliced transcript variants have been found for ATF2 (provided by RefSeq, Jan 2012).
Mouse anti Human ATF2 antibody detects bands of 65-75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti ATF2 antibody detects bands of approximately 86-75 kDa in A549 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Partial recombinant of human ATF2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg A549 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P15336 Related reagents
- Entrez Gene
- ATF2 Related reagents
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0008270 zinc ion binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043565 sequence-specific DNA binding
- GO:0045087 innate immune response
- GO:0046983 protein dimerization activity
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ATF2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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