APPL1 antibody | G03/2H2
siRNA Validated
Mouse anti APPL1
- Product Type
- PrecisionAb Monoclonal
- Clone
- G03/2H2
- Isotype
- IgG1
- Format
- Purified
- Specificity
- APPL1
Antibody Quality and Performance Guarantee
| Mouse anti Human APPL1 antibody recognizes APPL1, also known as DCC-interacting protein 13-alpha, AKT2 interactor or signaling adaptor protein DIP13alpha. The protein encoded by APPL1 has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus (provided by RefSeq, Jul 2008). Mouse anti Human APPL1 antibody detects a band of 83 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Mouse anti APPL1 antibody detects a band of approximately 83 kDa in HEK293 cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- E. coli-derived recombinant human APPL1 (aa 1-373)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
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| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q9UKG1
- Entrez Gene
- APPL1
- GO Terms
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0005792 microsome
- GO:0007049 cell cycle
- GO:0008283 cell proliferation
- GO:0008286 insulin receptor signaling pathway
- GO:0012506 vesicle membrane
- GO:0042981 regulation of apoptosis
- View More GO Terms
- GO:0031901 early endosome membrane
- GO:0043422 protein kinase B binding
- GO:0046324 regulation of glucose import
- GO:0090003 regulation of establishment of protein localization in plasma membrane
VMA00650
170517
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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