APEX1 antibody | OTI6E10

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Mouse anti APEX1

APEX1 antibody recognizes apurinic/apyrimidinic endodeoxyribonuclease 1 also known as AP endonuclease class I, AP lyase, deoxyribonuclase, protein REF-1 or redox factor-1.

APEX1 Antibody with Control Lysate

APEX1 antibody recognizes apurinic/apyrimidinic endodeoxyribonuclease 1 also known as AP endonuclease class I, AP lyase, deoxyribonuclase, protein REF-1 or redox factor-1.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI6E10
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00605 WB 100 µl
VMA00605KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human APEX1 antibody recognizes apurinic/apyrimidinic endodeoxyribonuclease 1 also known as AP endonuclease class I, AP lyase, deoxyribonuclase, protein REF-1 and redox factor-1 .

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein (provided by RefSeq, Jul 2008).

Mouse anti Human APEX1 antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti APEX1 antibody detects a band of approximately 34 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human APEX1 (aa 1-242)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P27695 Related reagents
Entrez Gene
APEX1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003684 damaged DNA binding
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0003723 RNA binding
GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
GO:0005739 mitochondrion
GO:0004523 ribonuclease H activity
GO:0004528 phosphodiesterase I activity
GO:0004844 uracil DNA N-glycosylase activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005840 ribosome
GO:0005730 nucleolus
GO:0005813 centrosome
GO:0016491 oxidoreductase activity
GO:0006284 base-excision repair
GO:0006310 DNA recombination
GO:0008408 3'-5' exonuclease activity
GO:0016607 nuclear speck
GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base
GO:0031490 chromatin DNA binding
GO:0043488 regulation of mRNA stability
GO:0045739 positive regulation of DNA repair
GO:0045768 positive regulation of anti-apoptosis
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of APEX1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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