Aldehyde Dehydrogenase 2 antibody | 4G6A3

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Anti Aldehyde Dehydrogenase 2 Antibody, clone 4G6A3 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Aldehyde Dehydrogenase 2 Antibody, clone 4G6A3 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Aldehyde Dehydrogenase 2 Antibody, clone 4G6A3 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Aldehyde Dehydrogenase 2 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Aldehyde Dehydrogenase 2 antibody (**VMA00235**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 70 second (chemiluminescent channel) and 30 second exposure

Anti Aldehyde Dehydrogenase 2 Antibody, clone 4G6A3 (PrecisionAb Monoclonal Antibody) gallery image 2 — Normalized signal intensity of Aldehyde Dehydrogenase 2 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Aldehyde Dehydrogenase 2 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti Aldehyde Dehydrogenase 2

Product Type: PrecisionAb Monoclonal
Clone: 4G6A3
Isotype: IgG1
Format: Purified
Specificity: Aldehyde Dehydrogenase 2

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00235 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human aldehyde dehydrogenase 2 antibody recognizes aldehyde dehydrogenase, mitochondrial, also known as ALDH class 2, acetaldehyde dehydrogenase 2, aldehyde dehydrogenase, mitochondrial, liver mitochondrial ALDH and nucleus-encoded mitochondrial aldehyde dehydrogenase 2.

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. ALDH2 encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Mar 2011).

Mouse anti Human aldehyde dehydrogenase 2 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti aldehyde dehydrogenase 2 detects a band of approximately 56 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant protein corresponding to amino acids 317-517 of human aldehyde dehydrogenase 2 expressed in E. coli
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P05091
Entrez Gene: ALDH2
GO Terms: GO:0009055 electron carrier activity; GO:0042136 neurotransmitter biosynthetic process; GO:0004029 aldehyde dehydrogenase (NAD) activity; GO:0005759 mitochondrial matrix; GO:0005975 carbohydrate metabolic process; GO:0006069 ethanol oxidation; GO:0006805 xenobiotic metabolic process