Actin antibody | AbD22606
Rhodamine conjugated anti actin hFAB antibody, clone AbD22606 (12004163) used as a loading control for western blottinng analysis ofcadherin protein expression in cell lysates.
Image caption:
Juxta-membrane lysine residues are needed for MARCH-induced downregulation of VE-cadherin. (B) Western blot analysis of cells expressing various cadherin mutants. Proteins are detected using antibodies directed against VE-cadherin, actin or GFP antibody.
From: Seo T, Lowery AM, Xu H, Giang W, Troyanovsky SM, Vincent PA, Kowalczyk AP.
MARCH family E3 ubiquitin ligases selectively target and degrade cadherin family proteins.
bioRxiv [Preprint]. 2023 Aug 10:2023.08.10.552739.
doi: 10.1101/2023.08.10.552739.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti actin hFAB™ Rhodamine Antibody (12004164) used as a loading control for western blotting analysis of reporter virus infected HuhCD300lf cell lysates.
Image caption:
Characterization of the reporter viruses. (E) The same copies of WT MNV or ΔNM-Venus virus (2 × 108 copies) were infected into HuhCD300lf cells (2 × 105 cells). Cell lysates were collected every 3 hours from 3 to 24 hpi. Actin was used as a loading control.
From: Ishiyama R, Yoshida K, Oikawa K, Takai-Todaka R, Kato A, Kanamori K, Nakanishi A, Haga K, Katayama K. Production of infectious reporter murine norovirus by VP2 trans-complementation. J Virol. 2024 Jan 16:e0126123 [Epub ahead of print].doi: 10.1128/jvi.01261-23.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rhodamine conjugated anti-actin hFAB™ antibody, clone AbD22606 (12004164) used as a loading control for the determination of protein abundance in cell line lysates by western blotting.
Image caption:
Protein abundance in SW48 cell line lysate with treatment. Western blot of the proteins MMUT, MTR and MAT2A compared to the loading control β actin (A). Quantified protein abundance relative to β-actin for MMUT (B), MTR(C), and MAT2A (D). CTL: Control, Cbl: cobalamin, HCY: homocystine
From: Garg, S.; Miousse, I.R.
Rescue of Methionine Dependence by Cobalamin in a Human Colorectal Cancer Cell Line.
Nutrients 2024, 16, 997.
doi: 10.3390/nu16070997
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
ANTI-ACTIN hFAB™ Rhodamine Antibody
- Product Type
- Monoclonal Antibody
- Clone
- AbD22606
- Isotype
- HuCAL Fab monovalent
- Specificity
- Actin
Antibody Quality and Performance Guarantee
| hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity. These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot. Features and Benefits Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
-
12004164
A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized -
12004163
A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized - Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
- Buffer Solution
- 12004164: Phosphate buffered saline.
- 12004163: Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% bovine serum albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Rhodamine 530 580 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
1 year at -20°C lyophilized; 6 months at +4°C after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
-
12004164, 12004163
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Actin antibody
-
Seo, T. et al. (2023) MARCH family E3 ubiquitin ligases selectively target and degrade cadherin family proteins.
bioRxiv. Aug 10 [Epub ahead of print]. -
Ishiyama, R. et al. (2024) Production of infectious reporter murine norovirus by VP2 trans-complementation.
J Virol. : e0126123. -
Garg, S. & , Miousse, I.R. (2024) Rescue of Methionine Dependence by Cobalamin in a Human Colorectal Cancer Cell Line
Nutrients. 16 (7): 997. -
Hyder, U. et al. (2024) KAP1 negatively regulates RNA polymerase II elongation kinetics to activate signal-induced transcription
bioRχiv 5 May [Preprint]
View more products with ACTIN specificity
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