Actin antibody | AbD22606

100% Secure


ANTI-ACTIN hFAB™ Rhodamine Antibody

Product Type
Monoclonal Antibody
Clone
AbD22606
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
12004164 WB 40 µl
12004163 WB 200 µl
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.

These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.

Features and Benefits

Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
Reconstitution
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
>80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin

Storage Information

Storage
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
Shelf Life
Please see label for expiry date

More Information

UniProt
P60709 Related reagents
Entrez Gene
ACTB Related reagents
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Actin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Goat ANTI-MOUSE IgG StarBright™ Blue 700 12004158 400 µl WB
Goat ANTI-RABBIT IgG StarBright™ Blue 700 12004161 400 µl WB
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004165 200 µl WB
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004166 40 µl WB
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004167 200 µl WB
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004168 40 µl WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB