FITC antibody | LO-FLUO-1

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Rat anti FITC:Biotin

Rat anti FITC

Product Type
Monoclonal Antibody
Clone
LO-FLUO-1
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1332B E F 0.5 mg
MCA1332 E F 0.5 mg
Rat anti FITC antibody, clone LO-FLUO-1 reacts with fluorescein isothiocyanate. Avidity against FITC conjugated BSA is 2 x 1010M-1.

This antibody binds to both Free FITC and FITC conjugated to proteins, and may be valuable for amplification of FITC based immunostaining.

Product Details

Target Species
Chemical
Product Form
Purified IgM conjugated to Biotin - liquid
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50%Glycerol
0.1%Sodium Azide
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
FITC conjugated mouse immunoglobulin.
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of FITC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Flow Cytometry
ELISA 1.25ug/ml 2.25ug/ml
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgM:Biotin 302008 1 mg C E IB
Goat anti Rat IgM:RPE 302009 0.5 mg F
Goat anti Rat IgM:HRP STAR116P 0.5 mg E WB

Product Specific References

References for FITC antibody

  1. Van Cauwenberge, A. et al. (2009) Development of a new low-cost and regenerable detection device for microbial compounds MIC-ATR
    Final Report Phase 1 Summary Brussels : Belgian Science Policy 2009 – 6 p. (Research Programme Science for a Sustainable Development) .