CD4 antibody | Du CD4-2

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Mouse anti Duck CD4

Product Type
Monoclonal Antibody
Clone
Du CD4-2
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2478 F IP P 0.25 mg
Mouse anti Duck CD4 antibody, clone Du CD4-2 recognizes Pekin duck CD4, shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells.

Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells (Kothlow et al. 2005).

Clone Du CD4-2 can be used to identify duck T helper cells. Mouse anti Duck CD4 antibody, clone Du CD4-2 does not appear to react with Mallard.

Product Details

Target Species
Duck
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
Immunogen
293T cells expressing Pekin duck CD4.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 10 ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD4 antibody

  1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
    Dev Comp Immunol. 29 (8): 733-48.
  2. Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.
    Vet Res. 43: 56.
  3. Shanmugasundaram, R. and Selvaraj, R.K. (2012) Regulatory T cell properties of thymic CD4(+)CD25(+) cells in ducks.
    Vet Immunol Immunopathol. 149: 20-7.
  4. Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
    Virol J. 8: 214.
  5. Huang, J. net al. (2014) An attenuated duck plague virus (DPV) vaccine induces both systemic and mucosal immune responses to protect ducks against virulent DPV infection.
    Clin Vaccine Immunol. 21: 457-62.
  6. Chen, S. et al. (2015) Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose.
    Immunobiology. pii: S0171-2985(14)00289-7.
  7. Zhou, H. et al. (2016) LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies
    Front Microbiol. Feb 17: [Epub ahead of print]
  8. Chen, S. et al. (2016) Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
    Int J Mol Sci. 17 (12): pii: E1990.
  9. Zhou H et al. (2016) Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ.
    Sci Rep. 6: 25545.
  10. Cornelissen, J.B. et al. (2013) Differences in highly pathogenic avian influenza viral pathogenesis and associated early inflammatory response in chickens and ducks.
    Avian Pathol. 42 (4): 347-64.

Further Reading

  1. Higgins, D.A. & Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.
    J Immunol Methods. 106 (1): 135-45.