CD4 antibody | Du CD4-2

Mouse anti Duck CD4

Product Type
Monoclonal Antibody
Clone
Du CD4-2
Isotype
IgG2a
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA2478
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP P 0.25 mg loader
List Price Your Price
loader
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Mouse anti Duck CD4 antibody, clone Du CD4-2 recognizes Pekin duck CD4, shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells.

Most avian immune research has been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin, and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells (Kothlow et al. 2005).

Clone Du CD4-2 can be used to identify duck T helper cells. Mouse anti Duck CD4 antibody, clone Du CD4-2 does not appear to react with Mallard.

Target Species
Duck
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
293T cells expressing Pekin duck CD4.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 10 ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)

References for CD4 antibody

  1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
    Dev Comp Immunol. 29 (8): 733-48.
  2. Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.
    Vet Res. 43: 56.
  3. Shanmugasundaram, R. and Selvaraj, R.K. (2012) Regulatory T cell properties of thymic CD4(+)CD25(+) cells in ducks.
    Vet Immunol Immunopathol. 149: 20-7.
  4. Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
    Virol J. 8: 214.
  5. Huang, J. net al. (2014) An attenuated duck plague virus (DPV) vaccine induces both systemic and mucosal immune responses to protect ducks against virulent DPV infection.
    Clin Vaccine Immunol. 21: 457-62.
  6. Chen, S. et al. (2015) Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose.
    Immunobiology. pii: S0171-2985(14)00289-7.
  7. Zhou, H. et al. (2016) LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies.
    Front Microbiol. 7: 166.
  8. Chen, S. et al. (2016) Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
    Int J Mol Sci. 17 (12): pii: E1990.
  9. View The Latest Product References
  10. Zhou H et al. (2016) Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ.
    Sci Rep. 6: 25545.
  11. Cornelissen, J.B. et al. (2013) Differences in highly pathogenic avian influenza viral pathogenesis and associated early inflammatory response in chickens and ducks.
    Avian Pathol. 42 (4): 347-64.
  12. Wu, Y. et al. (2019) Changes in the small intestine mucosal immune barrier in Muscovy ducklings infected with Muscovy duck reovirus
    Veterinary Microbiology. 233: 85-92.

Further Reading

  1. Higgins, D.A. & Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.
    J Immunol Methods. 106 (1): 135-45.

Flow Cytometry

RRID
AB_609597
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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