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IgM antibody | IL-A30

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Anti Bovine IgM Antibody, clone IL-A30 thumbnail image 1 Anti Bovine IgM Antibody, clone IL-A30 thumbnail image 2
Anti Bovine IgM Antibody, clone IL-A30 gallery image 1
Bovine IgM detected in bovine serum with Mouse anti Bovine IgM:HRP (MCA2443PA).
Anti Bovine IgM Antibody, clone IL-A30 gallery image 2
Bovine IgM whole molecule detected with Mouse anti Bovine IgM (MCA2443GA) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B).

Mouse anti Bovine IgM:HRP

Product Type
Monoclonal Antibody
Clone
IL-A30
Isotype
IgG1
Specificity
IgM

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Product Code Applications Pack Size List Price Your Price Qty
MCA2443PA
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E 0.1 mg loader
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Mouse anti Bovine IgM, clone IL-A30 recognizes bovine IgM, expressed by bovine B-cells. Clone IL-A30 reacts with both serum IgM and cell surface IgM expressed by B lymphocytes. Mouse anti Bovine IgM, clone IL-A30 recognizes a monomorphic epitope within bovine IgM (Naessens et al. 1988).

The percentage of IgM+ve lymphocytes may vary widely ,ranging between 4 - 30%, within populations of healthy cattle, however the percentage of positive cells remains relatively constant within individual animals (Naessens et al. 1988).

Target Species
Bovine
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
HRP Stabiliser (BUF052A)
0.01% Thiomersal
Immunogen
Bovine peripheral blood mononuclear cells depleted of T lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus

Source Reference

  1. Naessens, J. et al. (1988) Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies.
    Immunology. 63 (4): 569-74.

References for IgM antibody

  1. Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
    Vet Immunol Immunopathol. 24 (3): 267-83.
  2. Morrison, W.I. et al. (1996) Pathogenicity of Theileria parva is influenced by the host cell type infected by the parasite.
    Infect Immun. 64 (2): 557-62.
  3. Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
    J Leukoc Biol. 63 (5): 567-74.
  4. Naessens, J. (1997) Surface Ig on B lymphocytes from cattle and sheep.
    Int Immunol. 9 (3): 349-54.
  5. Menge, C. et al. (2004) Bovine ileal intraepithelial lymphocytes represent target cells for Shiga toxin 1 from Escherichia coli.
    Infect Immun. 72 (4): 1896-905.
  6. Nishimori, A. et al. (2016) Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection.
    J Vet Med Sci. 78 (5): 791-6.
  7. Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
    PLoS One. 11 (3): e0152192.
  8. Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
    PLoS One. 11 (9): e0162571.
    9. View The Latest Product References

MCA2443PA

0311R 150697

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