Browsing Modes for Business Users

CD21 antibody | CC21

ISO 9001 Quality Assurance Manufacturing | Print
Anti Bovine CD21 Antibody, clone CC21 thumbnail image 1 Anti Bovine CD21 Antibody, clone CC21 thumbnail image 2 Anti Bovine CD21 Antibody, clone CC21 thumbnail image 3 Anti Bovine CD21 Antibody, clone CC21 thumbnail image 4
Anti Bovine CD21 Antibody, clone CC21 gallery image 1
Figure A. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD8b (MCA1654PE) and FITC conjugated Mouse anti Bovine CD21 (MCA1424F). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD21 Antibody, clone CC21 gallery image 2
Figure A. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse anti Bovine CD21 (MCA1424F). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD21 Antibody, clone CC21 gallery image 3
Published customer image:
Mouse anti Bovine CD21 monoclonal antibody, clone CC21 (MCA1424GA) used to identify ovine CD21 expressing cells in heart tissue by immunofluorescence on cryostat sections.
Image caption:
Immunofluorescence examination of explanted heart valves. CD45‐positive cells could be found within (A) allogeneic and (B) xenogeneic explants. C, Allogeneic explants exhibited the lowest number of CD45‐positive cells, whereas xenogeneic (xeno) SDS/SD‐treated explants exhibited the highest number, about 40% of all invading cells. High numbers of CD11b‐positive cells were present in all (D) allogeneic and (E) xenogeneic explants; however, (F) no significant differences were found. G, In allogeneic explants, CD3‐positive T cells were almost absent, but in (H) xenogeneic explants, a low number of CD3‐positive cells could be found. I, Enzyme‐treated valves tended to contain less CD3‐positive cells as untreated explants. J, In allogeneic as well (K) xenogeneic explants, (L) only few CD21‐positive B‐cells could be found. C, F, I, L, Every dot represents one individual animal included in the analysis. For each PHV specimen, at least two sections obtained from different areas have been analyzed, counting 11 HPFs per section. All scale bars represent 100 μm.

From: Ramm R, Goecke T, Theodoridis K, Hoeffler K, Sarikouch S, Findeisen K, Ciubotaru A, Cebotari S, Tudorache I, Haverich A, Hilfiker A.
Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
Xenotransplantation. 2020 Mar;27(2):e12571.
doi: 10.1111/xen.12571.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine CD21 Antibody, clone CC21 gallery image 4
Published customer image:
Phycoerythrin conjugated Mouse anti Bovine CD21 monoclonal antibody, clone CC21 (MCA1424PE) used to discriminate lymphocyte populations in bovine blood samples by flow cytometry.
Image caption:
Changes in T and B lymphocytes among PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify lymphocytes subset population. Heat stress caused reduction in lymphocyte populations in the PBMCs of both Holstein and Jersey steers. The lymphocytes comprised CD21+MHCII+ B cells. MT indicates moderate THI condition and HT indicates high THI condition. * = P <0.05, ** = P <0.01

From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Filter by Application:

F IF C

Mouse anti Bovine CD21:FITC

Product Type
Monoclonal Antibody
Clone
CC21
Isotype
IgG1
Specificity
CD21

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA1424F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine CD21 monoclonal antibody, clone CC21 recognizes the bovine CD21 cell surface antigen, a ~145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessens et al. 1990). CD21 may be expressed on B cells as either a long or a short form (Pringle et al. 2012)

Mouse anti bovine CD21, clone CC21 has been used to demonstrate the co-expression of CD21 with PrPc on B cells of scrapie infected sheep (Halliday et al. 2005).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
Red deer
Mule deer
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD21 antibody

  1. Brodzki, P. et al. (2024) The influence of probiotic administration on selected leukocyte subpopulations and the serum amyloid A concentration in the peripheral blood of dairy cows during different lactation periods
    Journal of Veterinary Research. 09 Oct [Epub ahead of print].
  2. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes.
    Immunology 69: 525-30.
  3. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
    Vet Immunol Immunopathol. 27 (1-3): 21-7.
  4. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
    Vet Immunol Immunopathol. 81 (1-2): 85-96.
  5. Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer.
    J Gen Virol. 83: 2617-28.
  6. Kruger, E.F. et al. (2003) Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
    Dev Comp Immunol. 27 (10): 889-97.
  7. Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
    Immunology. 113: 310-7.
  8. Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
    J Gen Virol. 86 (Pt 5): 1571-9.
    9. View The Latest Product References
  9. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
    J Virol. 79: 7738-44.
  10. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
    Vet Pathol. 43: 127-35.
  11. Richt, J.A. et al. (2007) Production of cattle lacking prion protein.
    Nat Biotechnol. 25: 132-8.
  12. Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves.
    Cell Tissue Res. 338: 343-54.
  13. Ekman, A. et al. (2010) B-cell development in bovine fetuses proceeds via a pre-B like cell in bone marrow and lymph nodes.
    Dev Comp Immunol. 34 (8): 896-903.
  14. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
    Virology. 405: 110-9.
  15. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
    Vet Res Commun. 34: 197-203.
  16. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
    Vet Immunol Immunopathol. 137: 284-90.
  17. Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle.
    J Infect Dev Ctries. 4 (9): 576-9.
  18. Booth, J.S. et al. (2010) Co-stimulation with TLR7/8 and TLR9 agonists induce down-regulation of innate immune responses in sheep blood mononuclear and B cells.
    Dev Comp Immunol. 34 (5): 572-8.
  19. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
    Comp Immunol Microbiol Infect Dis. 34: 217-25.
  20. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
    Acta Histochem. 113: 416-22.
  21. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 146: 1-7.
  22. Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes.
    J Comp Pathol. 146: 365-71.
  23. Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow.
    Vet Rec. 170: 312.
  24. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
    Theriogenology. 82: 403-10.
  25. Meganck, V. et al. (2014) Development of a method for isolating bovine colostrum mononuclear leukocytes for phenotyping and functional studies.
    Vet J. 200: 294-8.
  26. Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
    PLoS One. 10 (8): e0136865.
  27. Nikbakht Brujeni, G. et al. (2016) Association of BoLA-DRB3.2 Alleles with BLV Infection Profiles (Persistent Lymphocytosis/Lymphosarcoma) and Lymphocyte Subsets in Iranian Holstein Cattle.
    Biochem Genet. 54 (2): 194-207.
  28. De Matteis G et al. (2016) Evaluation of leptin receptor expression on buffalo leukocytes.
    Vet Immunol Immunopathol. 177: 16-23.
  29. Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
    Vet Immunol Immunopathol. 206: 1-8.
  30. Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
    Vet Immunol Immunopathol. 211: 44-8.
  31. Brodzki, P. et al. (2020) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. 91 (1): e13306.
  32. Radley, G. et al. (2020) In Vitro. Benchmarking Study of Ventricular Assist Devices in Current Clinical Use.
    J Card Fail. 26 (1): 70-79.
  33. Okino, C.H. et al. (2020) A polymorphic CD4 epitope related to increased susceptibility to Babesia bovis. in Canchim calves.
    Vet Immunol Immunopathol. 230: 110132.
  34. Khosa, S. et al. (2020) Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations.
    Viruses. 12 (12): 1431.
  35. Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
    Infect Immun. 88 (3): e00521-19.
  36. Souza, F.N. et al. (2020) Lymphocyte proliferative responses in dairy cows supplemented with an immunomodulatory feed additive and administered polyvalent vaccination.
    Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 72 (6): 2397-401.
  37. Ramm, R. et al. (2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
    Xenotransplantation. 27 (2): e12571.
  38. Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
    Cell Stress Chaperones. 26 (4): 705-20.
  39. Colombatti Olivieri, M.A. et al. (2021) Evaluation of a virulent strain of Mycobacterium avium subsp. paratuberculosis used as a heat-killed vaccine.
    Vaccine. 39 (51): 7401-7412.
  40. Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
    Vet Immunol Immunopathol. 248: 110417.
  41. Okino, C.H. et al. (2022) CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification.
    Vet Immunol Immunopathol. 251: 110462.
  42. Andrés, S. et al. (2024) Essential oil supplementation in milk replacers: short- and long-term impacts on feed efficiency, the faecal microbiota and the plasma metabolome in dairy calves.
    J Dev Orig Health Dis. : 1-11.
  43. Hong, S. et al. (2024) Impact of an Injectable Trace Mineral Supplement on the Immune Response and Outcome of Mannheimia haemolytica Infection in Feedlot Cattle.
    Biol Trace Elem Res. Jun 10 [Epub ahead of print].
  44. Müller,.C.B.M. et al. (2024) Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves
    Animal Nutrition. 07 Sept [Epub ahead of print].

Flow Cytometry

Immunofluorescence

Synonyms
CR2
RRID
AB_323511

MCA1424F

151409 159935

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD21 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Bovine Products
Please note