CD1w2 antibody | CC20
Mouse anti Bovine CD1w2:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- CC20
- Isotype
- IgG2a
- Specificity
- CD1w2
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat Dog Horse Cat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Approx. Protein Concentrations
- Ig concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control:Alexa Fluor® 647 | ||||||
References for CD1w2 antibody
-
Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
Vet Immunol Immunopathol. 27 (1-3): 28-30. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.
Vet Immunol Immunopathol. 39 (1-3): 77-83. -
Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.
Am J Pathol. 148: 1699-708. -
Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
Vet Immunol Immunopathol. 60 (1-2): 15-31. -
Rhind, S.M. (2001) CD1--the pathology perspective.
Vet Pathol. 38 (6): 611-9. -
Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
Vet Pathol. 39: 74-83. -
Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72.
View The Latest Product References
-
Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.
Vet Immunol Immunopathol. 96: 19-30. -
McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
J Histochem Cytochem. 54: 1021-30. -
Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32: 837-49. -
Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
Vet Immunol Immunopathol. 131: 1-8. -
Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.
Vet Immunol Immunopathol. 129: 82-92. -
Romero-palomo, F. et al. (2013) Immunohistochemical detection of dendritic cell markers in cattle.
Vet Pathol. 50 (6): 1099-108. -
Romero-Palomo, F. et al. (2017) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
Transbound Emerg Dis. 64 (2): 574-84.
- Synonyms
- CD1b
MCA2058A647
152551
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