CD14 antibody | CAM36A
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD14 antibody, clone CAM36A (MCA6085) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD14 are plotted against bovine CD11b as recognized by FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F).
Mouse anti Bovine CD14, clone CAM36A, recognizes bovine CD14. CD14 is a co-receptor for bacterial lipopolysaccharide (LPS) and mediates the innate immune response. It is expressed at high levels on the surface of circulating monocytes, macrophages but also on bovine polymorphonuclear neutrophil leukocytes (PMN). Additionally, in cattle the soluble form of CD14 can be found present in the serum and milk (Sohn et al. 2004). CD14 signals through TLR4, MyD88, TIRAP and TRAF6 activating NFkB and eliciting the inflammatory response.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep Bison Water Buffalo Cat Dog Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Preparation
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Activated goat lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q95122
- Entrez Gene
- CD14
- GO Terms
- GO:0005886 plasma membrane
- GO:0006954 inflammatory response
- GO:0031225 anchored to membrane
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of CD14 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Functional Assays |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG1:RPE | STAR132PE | F | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine CD11c | MCA6084 | F IC | 0.1 mg |
|
Product Specific References
References for CD14 antibody
-
Ahn J.S. et al. (2002) Scavenger receptor cysteine-rich domains 9 and 11 of WC1 are receptors for the WC1 counter receptor.
J Leukoc Biol. 72 (2): 382-90. -
Zhang, Y. et al. (2001) Induction of interleukin-6 and interleukin-12 in bovine B lymphocytes, monocytes, and macrophages by a CpG oligodeoxynucleotide (ODN 2059) containing the GTCGTT motif.
J Interferon Cytokine Res. 21 (10): 871-81.
Fluorescent Spectraviewer
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- Select combined or multi-laser view to visualize the spectra
