CD14 antibody | CAM36A

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Mouse anti Bovine CD14

Product Type
Monoclonal Antibody
Clone
CAM36A
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6085 F 0.1 mg
Mouse anti Bovine CD14, clone CAM36A, recognizes bovine CD14. CD14 is a co-receptor for bacterial lipopolysaccharide (LPS) and mediates the innate immune response. It is expressed at high levels on the surface of circulating monocytes, macrophages but also on bovine polymorphonuclear neutrophil leukocytes (PMN). Additionally, in cattle the soluble form of CD14 can be found present in the serum and milk (Sohn et al. 2004). CD14 signals through TLR4, MyD88, TIRAP and TRAF6 activating NFkB and eliciting the inflammatory response.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
Bison
Water Buffalo
Cat
Dog
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Activated goat lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q95122 Related reagents
Entrez Gene
CD14 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0006954 inflammatory response
GO:0031225 anchored to membrane
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG1:RPE STAR132PE 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine CD11c MCA6084 0.1 mg F IC

Product Specific References

References for CD14 antibody

  1. Ahn J.S. et al. (2002) Scavenger receptor cysteine-rich domains 9 and 11 of WC1 are receptors for the WC1 counter receptor.
    J Leukoc Biol. 72 (2): 382-90.
  2. Zhang, Y. et al. (2001) Induction of interleukin-6 and interleukin-12 in bovine B lymphocytes, monocytes, and macrophages by a CpG oligodeoxynucleotide (ODN 2059) containing the GTCGTT motif.
    J Interferon Cytokine Res. 21 (10): 871-81.