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CD14 antibody | CAM36A

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Anti Bovine CD14 Antibody, clone CAM36A thumbnail image 1 Anti Bovine CD14 Antibody, clone CAM36A thumbnail image 2
Anti Bovine CD14 Antibody, clone CAM36A gallery image 1
Flow cytometric staining of bovine peripheral blood monocytes.
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD14 antibody, clone CAM36A (MCA6085) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD14 are plotted against bovine CD11b as recognized by FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F).
Anti Bovine CD14 Antibody, clone CAM36A gallery image 2
Published customer image:
Mouse anti Bovine CD14, clone CAM36A, (MCA6085E) used for the analysis of CD14 expression on ovine and bovine cells by flow cytometry.
Image caption:
Identification of neonatal intestinal mononuclear phagocytes in the ileal Peyer’s patch of lambs and calves. Intestinal tissues from the ileal Peyer’s patch (IPP) were recovered from 10-day-old lambs (n = 6) and calves (n = 10). Mechanical and enzymatic dissociations were performed to obtain total isolated intestinal cells, then stained for flow cytometry. Representative gating for mononuclear phagocytes (MP) subsets are shown for the IPP of lamb (A) and calf (B). Following exclusion of dead cells, CD11c+MHCII+ cells were gated to select total MP and then four subsets were distinguished based on surface expression of CD14, CD172α, CD11b, and Cadm1: CD14+CD172α+Cadm1intCD11b+ (subset 1, green), CD14−CD172α−Cadm1+CD11b− (subset 2, purple), CD14−CD172α+Cadm1intCD11b+ (subset 3, blue), and CD14−CD172α+/-Cadm1−CD11b− (subset 4, yellow). Subsets 1, 2, and 3 of MP from the IPP of a 10-day-old lamb were cell sorted by flow cytometry, cytospined, and stained with hematoxylin and eosin. Pictures of the three subsets of MP were obtained by optical microscopy (A, right panel). Pie charts represent the cell proportions in percentage of each intestinal MP subset among viable CD11c+MHCII+ cells (mean ± SD) for lambs (n = 6; A, bottom panel) and calves (n = 10; B, bottom panel).

From: Baillou A, Tomal F, Chaumeil T, Barc C, Levern Y, Sausset A, Pezier T, Schulthess J, Peltier-Pain P, Laurent F and Lacroix-Lamandé S
Characterization of intestinal mononuclear phagocyte subsets in young ruminants at homeostasis and during Cryptosporidium parvum infection.
Front. Immunol. (2024) 15:1379798.
doi: 10.3389/fimmu.2024.1379798
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Bovine CD14

Product Type
Monoclonal Antibody
Clone
CAM36A
Isotype
IgG1
Specificity
CD14

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Product Code Applications Pack Size List Price Your Price Qty
MCA6085
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine CD14, clone CAM36A, recognizes bovine CD14. CD14 is a co-receptor for bacterial lipopolysaccharide (LPS) and mediates the innate immune response. It is expressed at high levels on the surface of circulating monocytes, macrophages but also on bovine polymorphonuclear neutrophil leukocytes (PMN). Additionally, in cattle the soluble form of CD14 can be found present in the serum and milk (Sohn et al. 2004). CD14 signals through TLR4, MyD88, TIRAP and TRAF6 activating NFkB and eliciting the inflammatory response.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
Bison
Water Buffalo
Cat
Dog
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Activated goat lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG1:RPE STAR132PE F 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG1:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine CD11c MCA6084 F IC 0.1 mg
List Price Your Price
Description Mouse anti Bovine CD11c

References for CD14 antibody

  1. Ahn J.S. et al. (2002) Scavenger receptor cysteine-rich domains 9 and 11 of WC1 are receptors for the WC1 counter receptor.
    J Leukoc Biol. 72 (2): 382-90.
  2. Zhang, Y. et al. (2001) Induction of interleukin-6 and interleukin-12 in bovine B lymphocytes, monocytes, and macrophages by a CpG oligodeoxynucleotide (ODN 2059) containing the GTCGTT motif.
    J Interferon Cytokine Res. 21 (10): 871-81.
  3. Baillou, A. et al. (2024) Characterization of intestinal mononuclear phagocyte subsets in young ruminants at homeostasis and during Cryptosporidium parvum infection.
    Frontiers in Immunology. 15: 1379798. [Epub ahead of print].

UniProt
Q95122
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0006954 inflammatory response
GO:0031225 anchored to membrane
GO:0045087 innate immune response

MCA6085

142899 155892 159887 167049

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