Mouse anti Chicken Monocytes/Macrophages, clone KUL01 recognises chicken monocytes and macrophages. Clone KUL01 will also detect interdigitating cells and activated microglia but does not recognise Bu1+ve B-cells or CD3 +ve T-lymphocytes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Monocytes/Macrophages antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for Monocytes/Macrophages antibody
Mast, J. et al. (1998) Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01.
Vet Immunol Immunopathol. 61 (2-4): 343-57.
Wigley, P. et al. (2001) Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens.
Infect Immun. 69 (12): 7873-9.
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65.
Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
Dev Comp Immunol. 42 (2): 278-85.
Guabiraba, R. et al. (2017) Unveiling the participation of avian kinin ornithokinin and its receptors in the chicken inflammatory response.
Vet Immunol Immunopathol. 188: 34-47.
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