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Monocytes/Macrophages antibody | KUL01

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Anti Chicken Monocytes/Macrophages Antibody, clone KUL01 thumbnail image 1 Anti Chicken Monocytes/Macrophages Antibody, clone KUL01 thumbnail image 2
Anti Chicken Monocytes/Macrophages Antibody, clone KUL01 gallery image 1
Published customer image:
Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 (MCA5770) used for flow cytometry studies.
Image caption:
(C) Flow cytometric analysis of reporter transgene expression in KUL01+/CSF1R+ blood monocytes in a non-transgenic chicken, G1 cockerel MAA1-8:23 and representative G2 progeny from (B) that have either a full length or a partially-deleted insert.

From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM.
Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 2014 Aug;141(16):3255-65.
Anti Chicken Monocytes/Macrophages Antibody, clone KUL01 gallery image 2
Published investigator image:
Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 (MCA5770) used for Immunohistochemistry.
Image caption:
Immunohistochemical evidence of infiltration by T-cells (CD3+), B-cells (chB6+) and macrophages (Kul01+). Immunophenotyping identified the majority of infiltrating lymphocytes as being CD3-positive T-cells (A). They show a characteristic multifocal distribution pattern. With similar spatial characteristics, chB6-positive B-cells comprise the second largest fraction of infiltrating cells (B). Compared to the lymphocytes, the density of Kul01-positive macrophages appears much lower (C). The arrows point to immunopositive cells. All sections were prepared from the same region of the sciatic nerve. The orientation of the nerve fibres (NF) is indicated. Scale bar = 35μm.

From: Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp HC, Weigend S, Sharifi AR, Preisinger R, Schmahl W, Kaspers B, Matiasek K.
Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans.
J Neuroinflammation. 2010 Jan 28;7:7.

Mouse anti Chicken Monocytes/Macrophages

Product Type
Monoclonal Antibody
Clone
KUL01
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA5770
Datasheet
C F P*
MSDS
0.25 mg loader

Mouse anti Chicken Monocytes/Macrophages, clone KUL01 recognises chicken monocytes and macrophages. Clone KUL01 will also detect interdigitating cells and activated microglia but does not recognise Bu1+ve B-cells or CD3 +ve T-lymphocytes.

Product Details

Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Monocytes/Macrophages antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Monocytes/Macrophages antibody

  1. Mast, J. et al. (1998) Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01.
    Vet Immunol Immunopathol. 61 (2-4): 343-57.
  2. Wigley, P. et al. (2001) Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens.
    Infect Immun. 69 (12): 7873-9.
  3. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  4. Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
    Dev Comp Immunol. 42 (2): 278-85.
  5. Guabiraba, R. et al. (2017) Unveiling the participation of avian kinin ornithokinin and its receptors in the chicken inflammatory response.
    Vet Immunol Immunopathol. 188: 34-47.
Request a different product with the Monocytes/Macrophages specificity