MHC Class I antibody | F21-2

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Mouse anti Chicken MHC Class I

Product Type: Monoclonal Antibody
Clone: F21-2
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA5761 Datasheet	C F IP WB MSDS	0.25 mg	Log in		Get Quote

Mouse anti Chicken MHC Class 1 antibody, clone F21-2 recognizes Chicken MHC Class I expressed on the majority of nucleated cells.

Chicken MHC Class molecules (B-F and B-L molecules) are highly conserved, with tissue distribution, structure and functions being similar to their mammalian counterparts.Avian MHC Class I molecules, known also as B-F antigens, consist of a glycosylated polymorphic transmembrane α-chain non-covalently bound to the invariant β2-microglobulin subunit.

Mouse anti Chicken MHC Class I, clone F21-2 recognizes Turkey MHC Class I (Meyerhoff et al. 2012).

Product Details

Target Species

Chicken

Species Cross-Reactivity

Target Species	Cross Reactivity
Turkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by ion exchange chromatography from tissue culture supernatant

Buffer Solution

Borate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: Q6X3Z0
Regulatory: For research purposes only

Applications of MHC Class I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	E WB	0.5 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote

Product Specific References

References for MHC Class I antibody

Salomonsen, J. et al. (1991) Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system.
Proc Natl Acad Sci U S A. 88 (4): 1359-63.
Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
Poult Sci. 91: 383-92.
Walker, B.A. et al. (2011) The dominantly expressed class I molecule of the chicken MHC is explained by coevolution with the polymorphic peptide transporter (TAP) genes.
Proc Natl Acad Sci U S A. 108: 8396-401.
Crone, M. et al. (1985) Mouse monoclonal antibodies to class I and class II antigens of the chicken MHC. Evidence for at least two class I products of the B complex.
Immunogenetics. 21: 181-7.