MHC Class I antibody | F21-2
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Mouse anti Chicken MHC Class 1 antibody, clone F21-2 recognizes Chicken MHC Class I expressed on the majority of nucleated cells.
Chicken MHC Class molecules (B-F and B-L molecules) are highly conserved, with tissue distribution, structure and functions being similar to their mammalian counterparts.Avian MHC Class I molecules, known also as B-F antigens, consist of a glycosylated polymorphic transmembrane α-chain non-covalently bound to the invariant β2-microglobulin subunit.
Mouse anti Chicken MHC Class I, clone F21-2 recognizes Turkey MHC Class I (Meyerhoff et al. 2012).
Chicken MHC Class molecules (B-F and B-L molecules) are highly conserved, with tissue distribution, structure and functions being similar to their mammalian counterparts.Avian MHC Class I molecules, known also as B-F antigens, consist of a glycosylated polymorphic transmembrane α-chain non-covalently bound to the invariant β2-microglobulin subunit.
Mouse anti Chicken MHC Class I, clone F21-2 recognizes Turkey MHC Class I (Meyerhoff et al. 2012).
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q6X3Z0 Related reagents
- Regulatory
- For research purposes only
Applications of MHC Class I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | 0.5 mg | F | ||
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | 0.5 mg | E WB | ||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | 1 ml | F | ||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | 1 mg | F |
Product Specific References
References for MHC Class I antibody
-
Salomonsen, J. et al. (1991) Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system.
Proc Natl Acad Sci U S A. 88 (4): 1359-63. -
Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
Poult Sci. 91: 383-92. -
Walker, B.A. et al. (2011) The dominantly expressed class I molecule of the chicken MHC is explained by coevolution with the polymorphic peptide transporter (TAP) genes.
Proc Natl Acad Sci U S A. 108: 8396-401. -
Crone, M. et al. (1985) Mouse monoclonal antibodies to class I and class II antigens of the chicken MHC. Evidence for at least two class I products of the B complex.
Immunogenetics. 21: 181-7.
Further Reading
-
Kaufman, J. et al. (1989) B-G cDNA clones have multiple small repeats and hybridize to both chicken MHC regions.
Immunogenetics. 30 (6): 440-51.
