MHC Class I antibody | F21-2

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Mouse anti Chicken MHC Class I

Product Type
Monoclonal Antibody
Clone
F21-2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5761 C F IP WB 0.25 mg
Mouse anti Chicken MHC Class 1 antibody, clone F21-2 recognizes Chicken MHC Class I expressed on the majority of nucleated cells.

Chicken MHC Class molecules (B-F and B-L molecules) are highly conserved, with tissue distribution, structure and functions being similar to their mammalian counterparts.Avian MHC Class I molecules, known also as B-F antigens, consist of a glycosylated polymorphic transmembrane α-chain non-covalently bound to the invariant β2-microglobulin subunit.

Mouse anti Chicken MHC Class I, clone F21-2 recognizes Turkey MHC Class I (Meyerhoff et al. 2012).

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q6X3Z0 Related reagents
Regulatory
For research purposes only

Applications of MHC Class I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for MHC Class I antibody

  1. Salomonsen, J. et al. (1991) Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system.
    Proc Natl Acad Sci U S A. 88 (4): 1359-63.
  2. Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
    Poult Sci. 91: 383-92.
  3. Walker, B.A. et al. (2011) The dominantly expressed class I molecule of the chicken MHC is explained by coevolution with the polymorphic peptide transporter (TAP) genes.
    Proc Natl Acad Sci U S A. 108: 8396-401.
  4. Crone, M. et al. (1985) Mouse monoclonal antibodies to class I and class II antigens of the chicken MHC. Evidence for at least two class I products of the B complex.
    Immunogenetics. 21: 181-7.

Further Reading

  1. Kaufman, J. et al. (1989) B-G cDNA clones have multiple small repeats and hybridize to both chicken MHC regions.
    Immunogenetics. 30 (6): 440-51.