MHC Class I antibody | F21-2
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Mouse anti Chicken MHC Class I
- Product Type
- Monoclonal Antibody
- Clone
- F21-2
- Isotype
- IgG1
- Specificity
- MHC Class I
Antibody Quality and 
Unrivaled 
| Mouse anti Chicken MHC Class 1 antibody, clone F21-2 recognizes Chicken MHC Class I expressed on the majority of nucleated cells. Chicken MHC Class molecules (B-F and B-L molecules) are highly conserved, with tissue distribution, structure and functions being similar to their mammalian counterparts.Avian MHC Class I molecules, known also as B-F antigens, consist of a glycosylated polymorphic transmembrane α-chain non-covalently bound to the invariant β2-microglobulin subunit. Mouse anti Chicken MHC Class I, clone F21-2 recognizes Turkey MHC Class I (Meyerhoff et al. 2012). |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
References for MHC Class I antibody
-
Salomonsen, J. et al. (1991) Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system.
Proc Natl Acad Sci U S A. 88 (4): 1359-63. -
Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
Poult Sci. 91: 383-92. -
Walker, B.A. et al. (2011) The dominantly expressed class I molecule of the chicken MHC is explained by coevolution with the polymorphic peptide transporter (TAP) genes.
Proc Natl Acad Sci U S A. 108: 8396-401. -
Crone, M. et al. (1985) Mouse monoclonal antibodies to class I and class II antigens of the chicken MHC. Evidence for at least two class I products of the B complex.
Immunogenetics. 21: 181-7.
Further Reading
-
Kaufman, J. et al. (1989) B-G cDNA clones have multiple small repeats and hybridize to both chicken MHC regions.
Immunogenetics. 30 (6): 440-51.
- RRID
- AB_10730837
- UniProt
- Q6X3Z0
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