Hen Egg Lysozyme antibody | AbD11397
Lysozyme damages bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk and mucus. It is also present in cytoplasmic granules of PMN’s and high concentrations of lysozyme are present in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family.
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5-tag (GSGGAPGKPIPNPLLGLDSTDAP) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Lysozyme from chicken egg white.
- Approx. Protein Concentrations
- Total protein concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00698
- Entrez Gene
- LYZ
- GO Terms
- GO:0003796 lysozyme activity
- GO:0042742 defense response to bacterium
- GO:0005615 extracellular space
- GO:0019835 cytolysis
- GO:0016998 cell wall macromolecule catabolic process
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Regulatory
- For research purposes only
Applications of Hen Egg Lysozyme antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1ug/ml | 0.1µg/ml |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Alexa Fluor® 488 | MCA1396A488 | F | 100 Tests/1ml |
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Mouse anti Histidine Tag:Alexa Fluor® 647 | MCA1396A647 | F | 1 ml |
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Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Mouse anti Histidine Tag:FITC | MCA1396F | F IF | 0.1 mg |
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
HuCAL Fab-V5H Negative Control | HCA098A | E | 50 µg |
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