Hen Egg Lysozyme antibody | AbD11397
Human anti Chicken Hen Egg Lysozyme, clone AbD11397, specifically recognises Hen Egg Lysozyme (HEL), known also as muramidase or N-acetylmuramide glycanhydrolase, a 14kDa enzymic protein involved in the destruction of bacteria.
Lysozyme damages bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk and mucus. It is also present in cytoplasmic granules of PMN’s and high concentrations of lysozyme are present in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family.
Lysozyme damages bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk and mucus. It is also present in cytoplasmic granules of PMN’s and high concentrations of lysozyme are present in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family.
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5-tag (GSGGAPGKPIPNPLLGLDSTDAP) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Lysozyme from chicken egg white.
- Approx. Protein Concentrations
- Total protein concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00698
- Entrez Gene
- LYZ
- GO Terms
- GO:0003796 lysozyme activity
- GO:0042742 defense response to bacterium
- GO:0005615 extracellular space
- GO:0019835 cytolysis
- GO:0016998 cell wall macromolecule catabolic process
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences. - Regulatory
- For research purposes only
Applications of Hen Egg Lysozyme antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1ug/ml | 0.1µg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
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