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Hen Egg Lysozyme antibody | AbD11397

Human anti Chicken Hen Egg Lysozyme

Product Type
Monoclonal Antibody
Clone
AbD11397
Isotype
HuCAL Fab monovalent
Specificity
Hen Egg Lysozyme

Product Code Applications Pack Size List Price Your Price Qty
HCA139
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader
HCA139A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µg loader
List Price Your Price
loader

Human anti Chicken Hen Egg Lysozyme, clone AbD11397, specifically recognises Hen Egg Lysozyme (HEL), known also as muramidase or N-acetylmuramide glycanhydrolase, a 14kDa enzymic protein involved in the destruction of bacteria.

Lysozyme damages bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk and mucus. It is also present in cytoplasmic granules of PMN’s and high concentrations of lysozyme are present in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family.

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a V5-tag (GSGGAPGKPIPNPLLGLDSTDAP) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Lysozyme from chicken egg white.
Approx. Protein Concentrations
Total protein concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1ug/ml 0.1µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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Synonyms
HEL
RRID
AB_10612253
UniProt
P00698
Entrez Gene
LYZ
GO Terms
GO:0003796 lysozyme activity
GO:0042742 defense response to bacterium
GO:0005615 extracellular space
GO:0019835 cytolysis
GO:0016998 cell wall macromolecule catabolic process

HCA139

151621

HCA139A

151622 165667 166853

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