Hen Egg Lysozyme antibody | AbD11397
Human anti Chicken Hen Egg Lysozyme, clone AbD11397, specifically recognises Hen Egg Lysozyme (HEL), known also as muramidase or N-acetylmuramide glycanhydrolase, a 14kDa enzymic protein involved in the destruction of bacteria.
Lysozyme damages bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk and mucus. It is also present in cytoplasmic granules of PMN’s and high concentrations of lysozyme are present in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family.
Lysozyme damages bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk and mucus. It is also present in cytoplasmic granules of PMN’s and high concentrations of lysozyme are present in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family.
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5-tag (GSGGAPGKPIPNPLLGLDSTDAP) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
- Reconstitution
- Pack Size: 50 µgReconstitute with 0.5ml distilled waterPack Size: 0.1 mgReconstitute with 1.0ml distilled water
- Preparation
- metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Lysozyme from chicken egg white.
- Approx. Protein Concentrations
- Total protein concentration 0.1mg/ml
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P00698 Related reagents
- Entrez Gene
- LYZ Related reagents
- GO Terms
- GO:0003796 lysozyme activity
- GO:0042742 defense response to bacterium
- GO:0005615 extracellular space
- GO:0019835 cytolysis
- GO:0016998 cell wall macromolecule catabolic process
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Regulatory
- For research purposes only
Applications of Hen Egg Lysozyme antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1ug/ml | 0.1µg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | 0.5 ml | E WB | ||
| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | 0.1 mg | E WB | ||
| Mouse anti Histidine Tag:Alexa Fluor® 488 | MCA1396A488 | 100 Tests/1ml | F | ||
| Mouse anti Histidine Tag:Alexa Fluor® 647 | MCA1396A647 | 1 ml | F | ||
| Mouse anti Histidine Tag:Biotin | MCA1396B | 0.1 mg | E WB | ||
| Mouse anti Histidine Tag:FITC | MCA1396F | 0.1 mg | F IF | ||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E WB |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| HuCAL Fab-V5H Negative Control | HCA098A | 50 µg | C E F P WB |
