CD25 antibody | AbD13504ka

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Human anti Chicken CD25

Product Type: Monoclonal Antibody
Clone: AbD13504ka
Isotype: HuCAL/mouse IgG1 Fc
Specificity: CD25

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References (5)
Applications
Storage Information
Batch/Lot Specific Datasheets

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HCA458 Datasheet SDS SDS	E F	0.1 mg		Log in
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Anti Chicken CD25 Antibody, clone AbD13504ka thumbnail image 1

Anti Chicken CD25 Antibody, clone AbD13504ka gallery image 1 — Flow cytometry analysis of chicken PBMCs stained with Anti-Chicken CD4 RPE, (MCA2164PE) and Anti-Mouse IgG1 isotype control purified (MCA928) detected with Anti-Mouse IgG1 FITC (STAR132F) only (Figure A) or Anti Chicken CD4 RPE (MCA2164PE) and Anti-Chicken CD25 (**HCA458**) detected with Anti-Mouse IgG1 FITC (STAR132F) (Figure B). Experiments performed on Chicken PBMCs stimulated with 2.5ug of ConA gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.

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Human anti Chicken CD25 antibody, clone AbD13504ka, recognizes the chicken IL-2 receptor alpha chain (IL-2Rα).

Chicken IL-2R&alpha, cited as ChCD25 in published literature, is a cell surface antigen expressed by activated T-lymphocytes, as well as monocytes/macrophages and thrombocytes.

Chicken IL-2Rα forms part of the high-affinity IL-2 receptor complex when associated with IL-2Rβ and γc, but alone it represents the low-affinity binding component for IL-2. This receptor plays a central role in T cell activation, proliferation, and immune regulation, including the maintenance of peripheral tolerance through regulatory T cells. Recent studies highlight that CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) suggesting an important role in the immune response to avian influenza virus infection (Huang et al. 2012).

Human anti Chicken CD25, clone AbD13504ka, is derived from clone AbD13504 (HCA173), and is a full length chimeric human/mouse antibody.

Target Species: Chicken
Product Form: Recombinant chimeric human/mouse IgG1 antibodyselected from the HuCAL phage display library, expressed in in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. This antibody is supplied as a liquid.
Preparation: Metal chelate affinity chromatography
Source: HKB-11
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinantly expressed his-tagged chicken CD25
Approx. Protein Concentrations: Antibody concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.
Licensed Use: For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

Source Reference

Braukmann, M. et al. (2015) Avian CD25(+) gamma/delta (γδ) T cells after Salmonella exposure.
Vet Immunol Immunopathol. 168 (1-2): 14-8.
Polasky C et al. (2016) Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.
Vet Immunol Immunopathol. 179: 1-7.
Lee, I.K. et al. (2018) Regulation of CD4⁺CD8^-CD25⁺ and CD4⁺CD8⁺CD25⁺ T cells by gut microbiota in chicken.
Sci Rep. 8 (1): 8627.
He, S. et al. (2021) High-frequency and activation of CD4⁺CD25⁺ T cells maintain persistent immunotolerance induced by congenital ALV-J infection.
Vet Res. 52 (1): 119.
Rimondi, A. et al. (2022) Few Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultry.
Front Microbiol. 13: 953738.

Synonyms: IL-2 Receptor Alpha
UniProt: Q90WJ4